161,261 results

1. pLPCX roGFP2-Orp1

   Plasmid

   #64991

   Purpose

   Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-EGFP-C1-HsNot1

   Plasmid

   #37370

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsNot1 (CNOT1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCVpuro-mTagBFP2

   Plasmid

   #96935

   Purpose

   Retroviral plasmid containing mTagBFP2 for labeling of cells

   Depositor

   Lee Grimes

   Article

   Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

   Insert

   Monomeric Tag Blue Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. mOrange2-pBAD

   Plasmid

   #54531

   Purpose

   Localization: Protein Expression Vector , Excitation: 549, Emission: 565

   Depositor

   Roger Tsien, Nathan Shaner, Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Use

   Tags

   6xHis and mOrange2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29a-YS14

   Plasmid

   #66890

   Purpose

   Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

   Depositor

   Jung-Hyun Min

   Article

   Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

   Inserts

   His6-Thrombin-Xenopus laevis histones H2A
   Xenopus laevis histones H2B
   Xenopus laevis histones H3
   Xenopus laevis histones H4-Thrombin
   

   Use

   Tags

   H2A, H2B, H3, H4, His6 tag, and Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-DIO-JEDI-2P-WPRE

   Plasmid

   #179460

   Purpose

   Double floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector under the control of the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Has Service

   AAV1

   Insert

   JEDI-2P
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-HyPer7-MEM

   Plasmid

   #136465

   Purpose

   Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Farnselation tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF Con/Fon DREADD Gq-mCherry

   Plasmid

   #183532

   Purpose

   INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

   Depositor

   Karl Deisseroth

   Insert

   DREADD Gq-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   nEF

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBR1.cas9-NT

   Plasmid

   #197871

   Purpose

   Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

   Depositor

   Baojun Wang

   Article

   Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

   Inserts

   trancCRNA
   Cas9
   crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBUN6I11

    Plasmid

    #50580

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-KRAB
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Expression

    Mutation

    dCas9 that is defective in DNA cleavage; the maiz…

    Promoter

    OsU3p and Ubi1p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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