4,168 results

1. LV-RFP

   Plasmid

   #26001

   Depositor

   Elaine Fuchs

   Article

   Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

   Insert

   H2B-RFP (H2BC21 Human, A. victoria)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKMyc

   Plasmid

   #19400

   Depositor

   Ian Macara

   Article

   Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

   Type

   Empty backbone

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   April 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. MaLionR

   Plasmid

   #113908

   Purpose

   Red ATP sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

   Insert

   MaLionR
   

   Expression

   Mammalian

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-hGal3

   Plasmid

   #73080

   Purpose

   Expression in mammalian cells

   Depositor

   Tamotsu Yoshimori

   Article

   Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

   Insert

   Galectin-3 (LGALS3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-TRAIL

   Plasmid

   #10953

   Depositor

   Bingliang Fang

   Article

   Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

   Insert

   Tumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. TurboID-His6_pET21a

   Plasmid

   #107177

   Purpose

   expresses His6-tagged TurboID in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Bacterial

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-CMV-mCherry-T2A-Puro

   Plasmid

   #72264

   Purpose

   Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

   Depositor

   Kazuhiro Oka

   Insert

   mCherry-T2A-Puro
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-2xFLAG-SREBP-1a

   Plasmid

   #26801

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   SREBP-1a (SREBF1 Human)
   

   Tags

   2xFLAG

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2/HB

    Plasmid

    #26557

    Depositor

    Perry Hackett

    Insert

    pT2/HB
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMKV057

    Plasmid

    #133312

    Purpose

    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPT

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc+. WUS2, IPT
    

    Expression

    Plant

    Promoter

    AtUbi10, Nos, 35S

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAKgfp1

    Plasmid

    #14076

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Green fluorescent protein
    

    Expression

    Bacterial

    Available Since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #180280

    Purpose

    The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (backbone) and BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ230 (LbCpf1)

    Plasmid

    #86210

    Purpose

    LbCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    5' and 3' NLS

    Expression

    Plant

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFPC1-hMyoX

    Plasmid

    #47608

    Depositor

    Emanuel Strehler

    Article

    Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

    Insert

    MyoX (MYO10 Human)
    

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHCMV-AmphoEnv

    Plasmid

    #15799

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    4070A Amphotropic Murine leukemia virus envelope gene
    

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-lyso-pHluorin

    Plasmid

    #70113

    Purpose

    Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHluorin (Cd63 Mouse, Aequorea victoria)
    

    Tags

    HA-Tag and pHluorin

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG.PKCepsilon

    Plasmid

    #10795

    Depositor

    Alex Toker

    Article

    Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

    Insert

    PKC epsilon (PRKCE Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-blast-SCRAMBLE

    Plasmid

    #26701

    Purpose

    3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223 SARS-CoV-2 NSP6

    Plasmid

    #141260

    Purpose

    Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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