4,133 results

1. pcDNA3-ADAM17-HA

   Plasmid

   #65105

   Purpose

   Expression of HA tagged ADAM17 (TACE) in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   ADAM17 (ADAM17 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-FLEX-GFP

   Plasmid

   #28304

   Depositor

   Edward Boyden

   Insert

   GFP
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   David Sabatini, Eric Lander

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti PGK Puro DEST (w529-2)

   Plasmid

   #19068

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBAD-mTagBFP2

   Plasmid

   #34632

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I174A compared to mTagBFP (enhanced photostabilit…

   Promoter

   Available since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV_BE4max

   Plasmid

   #112093

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   BE4max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV SOCS-3

   Plasmid

   #11486

   Depositor

   Ronald Kahn

   Article

   Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

   Insert

   SOCS-3 (Socs3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQCXIP-BSR-GFP11

   Plasmid

   #68716

   Purpose

   Split GFP assay

   Depositor

   Yutaka Hata

   Article

   Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

   Insert

   bsr
   

   Use

   Retroviral

   Tags

   FLAG and GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti6.2_miRFP670

    Plasmid

    #113726

    Purpose

    Expresses the fluorescent protein miRFP670 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    miRFP670
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSBtet-GP

    Plasmid

    #60495

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TCE & RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBB540

    Plasmid

    #27393

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    grpE, clpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMBP-AsCas12a

    Plasmid

    #113430

    Purpose

    Expresses 10xHis-MBP fused to AsCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AsCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PGC-1 alpha promoter 2kb luciferase

    Plasmid

    #8887

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET His6 TEV LIC cloning vector (1B)

    Plasmid

    #29653

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CLYBL-TO-hNGN2-BSD-mApple

    Plasmid

    #124229

    Purpose

    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

    Depositor

    Michael Ward

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV4-ApoE3

    Plasmid

    #87086

    Purpose

    Expresses ApoE3 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E3 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-C1-FLAG

    Plasmid

    #46956

    Purpose

    Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    FLAG tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Fuw-dCas9-Tet1CD

    Plasmid

    #84475

    Purpose

    Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-KG-D4H*-mCherry

    Plasmid

    #134604

    Purpose

    fluorescent cholesterol-binding probe

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    D4H*
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    Y415A and A463W

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge