We narrowed to 3,429 results for: Agt

1. pSicoR-Ef1a-mCh-Puro-Luci

   Plasmid

   #31850

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   Luciferase RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP1341

   Plasmid

   #119272

   Purpose

   control sgRNA in cells lacking rfp

   Depositor

   Jason Peters, Oren Rosenberg, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458_ARID5B_1

   Plasmid

   #86350

   Purpose

   Encodes gRNA for 3' target of human ARID5B

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against ARID5B (ARID5B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSMP-MeCP2_1

   Plasmid

   #36377

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   MeCP2 (MECP2 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

5. CENPF C5.1 gRNA

   Plasmid

   #90616

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CENPF

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CENPF (Guide Designation C5.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEasyG2_mic

   Plasmid

   #184916

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-shAUF1 hairpin 1

   Plasmid

   #52921

   Purpose

   expresses a hairpin specific for human AUF1

   Depositor

   Sheila Stewart

   Article

   Alspach et al Cancer Discov. 2014 Mar 26.

   Insert

   shRNA targeting AUF1 (HNRNPD Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 9, 2014

   Availability

   Academic Institutions and Nonprofits only

8. TUBG1 F7.4 gRNA

   Plasmid

   #90930

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TUBG1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   TUBG1 (Guide Designation F7.4)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSPneogRNA241510+MT

   Plasmid

   #84292

   Purpose

   Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA
   

   Use

   CRISPR; Leishmania donovani

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ1371-sgITGB4-1

    Plasmid

    #121535

    Purpose

    sgITGB4-1 sequence: GAGGCGCAGTCCTTATCCACA. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Stanley Qi, Ovijit Chaudhuri

    Article

    Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

    Insert

    sgITGB4-1
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCAS9n(BB)-2A-puro DNM1, B

    Plasmid

    #107796

    Purpose

    gRNA vector for knock-in gene editing at human DNM1 locus

    Depositor

    Sandra Schmid

    Article

    Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

    Insert

    gRNA targeting human DNM1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PPP1CB C3.5 gRNA

    Plasmid

    #90845

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PPP1CB

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PPP1CB (Guide Designation C3.5)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458_ARID5B_2

    Plasmid

    #86351

    Purpose

    Encodes gRNA for 3' target of human ARID5B

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against ARID5B (ARID5B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-shAUF1 hairpin 2

    Plasmid

    #52922

    Purpose

    expresses a hairpin specific for human AUF1

    Depositor

    Sheila Stewart

    Article

    Alspach et al Cancer Discov. 2014 Mar 26.

    Insert

    shRNA targeting AUF1 (HNRNPD Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

15. ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA

    Plasmid

    #178110

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G6 T804N pegRNA + MTOR-E2419K pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRIP13 H3.2 gRNA

    Plasmid

    #90920

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIP13

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    TRIP13 (Guide Designation H3.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458_CEBPG_1

    Plasmid

    #86344

    Purpose

    Encodes gRNA for 3' target of human CEBPG

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against CEBPG (CEBPG Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSMP-MeCP2_3

    Plasmid

    #36379

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MeCP2 (MECP2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pAT15416-BEAR-mScarlet-target-BFP

    Plasmid

    #162996

    Purpose

    plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    sgRNA targeting the BEAR-mScarlet plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. LRG-2.1T-neo-sgROSA

    Plasmid

    #105587

    Purpose

    lentivirally express gRNA targeting ROSA26 locus with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    gRNA targeting rosa26 locus
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOPTXcGMPRELUC

    Plasmid

    #68503

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    

    Use

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

    Promoter

    OPTX promoter with cGMPRE

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3.1(+)-MC38-TCRB-32-riot_punisher

    Plasmid

    #138988

    Purpose

    IVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1(+)-MC38-TCRA-32-riot_punisher

    Plasmid

    #138987

    Purpose

    IVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1(+)-MC38-TCRB-30-picky_sticky

    Plasmid

    #138984

    Purpose

    IVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pB-puro-mU6-CNPshRNA1

    Plasmid

    #126611

    Purpose

    Expresses shRNA against human CNP under mouse U6 promoter

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    CNP ShRNA-1 (CNP Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pX330_ATP1A1_G3_Dual_sgRNA

    Plasmid

    #173205

    Purpose

    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

    Plasmid

    #173214

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
    

    Use

    CRISPR; Prime editing (pe3)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

    Plasmid

    #124859

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSC50

    Plasmid

    #104824

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.08g081600, Glyma.05g126600
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO-sh-Myocardin

    Plasmid

    #100769

    Purpose

    Lentiviral expression of shRNA targeting MYOCD

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    Lenti-sh-Myocardin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge