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Plasmid#192636PurposeExpresses ERT2-CreN59-nMag-NLS with mCherry marker.DepositorInsertERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145-ZmUbi-tRNA
Plasmid#158403PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-G-GECO1.0
Plasmid#32447PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)DepositorInsertG-GECO1.0
UseTagsExpressionMammalianMutationGCaMP3 K119I/L173Q/S404G/E430VPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYPQ143-ZmUbi-tRNA
Plasmid#158400PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-GINKO1
Plasmid#113111PurposeBacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1DepositorInsertGINKO1
UseTags6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…ExpressionBacterialMutationPromoteraraBADAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SRY_P2A_Hygro_Barcode
Plasmid#120485PurposeBarcoded lentiviral vector to express SRY in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSRY (SRY Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-SPW-RHH
Plasmid#190630PurposeBacterial expression of genetically encoded protease indicator SPW-RHHDepositorInsertSPW-RHH
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135B2.0
Plasmid#167159PurposeGolden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-PUM1-rABE-Flag
Plasmid#191385PurposeDoxycycline inducible expression of PUM1-rABEDepositorInsertPUM1-rABE (PUM1 Human, Synthetic)
UseLentiviralTagsFlagExpressionMutationPromotertet-inducible promoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a_OTX2_P2A_Hygro_Barcode
Plasmid#120471PurposeBarcoded lentiviral vector to express OTX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertOTX2 (OTX2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miRFP713-P2A-GFP
Plasmid#197163PurposeExpresses the protein of miRFP713-P2A-GFP in mammalian cellsDepositorInsertmiRFP713-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAOJ15
Plasmid#91567PurposeBroad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterTetracycline promoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ146-ZmUbi-tRNA
Plasmid#158404PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSGKP_AcsgRNA
Plasmid#203807PurposepSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.DepositorInsertAcgRNA
UseCRISPRTagsExpressionMutationPromoterJ23119Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136B2.0
Plasmid#167160PurposeGolden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ144-ZmUbi-tRNA
Plasmid#158402PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mNeptune2.5-P2A-GFP
Plasmid#197156PurposeExpresses the protein of mNeptune2.5-P2A-GFP in mammalian cellsDepositorInsertmNeptune2.5-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gal4-PGC1 alpha
Plasmid#8892DepositorInsertPGC-1 alpha (Ppargc1a Mouse)
UseTagsGal4 DBDExpressionMammalianMutationPromoterAvailable sinceApril 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
KA1153_pPBCAG-MerCreMer-IN
Plasmid#124183PurposepiggyBac-based expression of the tamoxifen-inducible CreDepositorInsertMerCreMer-IRES-Neo
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLN592 (FuGW-S(USF1)p-mKate2)
Plasmid#105204Purposesynthetic promoter USF1 drives mKate2 expressionDepositorInsertS(USF1)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLN382 (FuGW-S(HIF1A)p-mKate2 )
Plasmid#105180Purposesynthetic promoter HIF1A drives mKate2 expressionDepositorInsertS(HIF1A)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version2
Plasmid#197932PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTags2xNLS-2xFLAGExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_PAX7_P2A_Hygro_Barcode
Plasmid#120472PurposeBarcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertPAX7 (PAX7 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1
Plasmid#32466DepositorInsertG-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…ExpressionBacterialMutationGCaMP3 K119I/L173Q/S404G/E430VPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
MTK2_001
Plasmid#123697PurposeEncodes pTRE as a Type 2 part to be used in the MTK systemDepositorInsertpTRE
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11274:pHR-hU6-crTet-HSP-hyperdCas12a-miniVPR-CMV-mCherry
Plasmid#210603Purposeheat shock promoter - activated hyperdCas12a-based transcriptional activatorDepositorInserthyperdCas12a-miniVPR
UseCRISPR and LentiviralTagsExpressionMutationPromotertruncated hsp70Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SPIB_P2A_Hygro_Barcode
Plasmid#120483PurposeBarcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSPIB (SPIB Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only