We narrowed to 4,065 results for: p2a

1. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-Rncp-iGluSnFR1

   Plasmid

   #187093

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Pre-SPHERE-Rncp-iGluSnFR1
   

   Use

   Tags

   TagBFP-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-iRFP-P2A-Post-SPHERE-Rncp-iGluSnFR1

   Plasmid

   #187094

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Post-SPHERE-Rncp-iGluSnFR1
   

   Use

   Tags

   iRFP670-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-mCherry-P2A-Pre-SPHERE-sfmTurquoise2-iGluSnFR

   Plasmid

   #187095

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Pre-SPHERE-sfmTurquoise2-iGluSnFR
   

   Use

   Tags

   mCherry-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

   Plasmid

   #169592

   Purpose

   Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   phloretin-controlled SEAP and iRFP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TtgO2-PCMVmin-2

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670

   Plasmid

   #169667

   Purpose

   Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   vanillic acid-controlled SEAP and iRFP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   VanO2-PCMVmin

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)

   Plasmid

   #170488

   Purpose

   SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

   Insert

   FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC19-TgKI-MCS-p2A-QF2-MCS-polyA

   Plasmid

   #174026

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-QF2
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIE061 ZF43x6-C mKate2 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138742

   Purpose

   Integration Vector, assembled with pHIE045 (TUPV1), pJM451 (TUPV2), pPD612

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHIE065 ZF43x12-S mKate2 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138746

   Purpose

   Integration Vector, assembled with pHIE049 (TUPV1), pJM451 (TUPV2), pPD612

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x12-Spaced
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHIE059 ZF43x1 mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138740

    Purpose

    Integration Vector, assembled with pHIE043 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHIE060 ZF43x3-C mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138741

    Purpose

    Integration Vector, assembled with pHIE044 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x3-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHIE063 ZF43x3-S mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138744

    Purpose

    Integration Vector, assembled with pHIE047 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x3-Spaced
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHIE064 ZF43x6-S mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138745

    Purpose

    Integration Vector, assembled with pHIE048 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Spaced
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHIE062 ZF43x12-C mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138743

    Purpose

    Integration Vector, assembled with pHIE046 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x12-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid

    #121804

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

    Plasmid

    #121808

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh1

    Plasmid

    #123324

    Purpose

    Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF3 sh1

    Plasmid

    #123326

    Purpose

    Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh2

    Plasmid

    #123325

    Purpose

    Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZIP-hCMV-ZSGreen-P2A-Puro-IKZF3 sh2

    Plasmid

    #123327

    Purpose

    Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

    Plasmid

    #118808

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All F to S in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 N-Q]

    Plasmid

    #107847

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All asparagine to glutamine in splicing reporter

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHBS1185 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

    Plasmid

    #107843

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All phenylalanine to serine in splicing reporter

    Promoter

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMiR-CIP2A-stop-mut-miR-375-mutA-E

    Plasmid

    #53714

    Purpose

    Luciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutations

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on the stop codon of firefly luciferase …

    Promoter

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

25. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

    Plasmid

    #190112

    Purpose

    AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Inserts

    MMLV-RT(dRH)
    U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
    

    Use

    AAV

    Tags

    bpNLS and bpNLS-P2A-eGFP

    Expression

    Mutation

    mutations from RT in PE2 and truncation of RNAse …

    Promoter

    EFS and U6, H1

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

    Plasmid

    #226514

    Purpose

    Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

    Plasmid

    #60910

    Purpose

    dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    dSV40 Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid

    #217971

    Purpose

    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.

    Depositor

    Robert Piper

    Insert

    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA

    Plasmid

    #177576

    Purpose

    Expresses SOUL in neuronal cell types

    Depositor

    Guoping Feng

    Article

    Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

    Insert

    SOUL
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-EF1a-FLEX-SOUL-P2A-tdTomato-WPRE-BGHpA

    Plasmid

    #177577

    Purpose

    Expresses SOUL in Cre-positive cells

    Depositor

    Guoping Feng

    Article

    Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

    Insert

    SOUL
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge