4,076 results

1. Tet-O-FUW-Neurod1

   Plasmid

   #30129

   Depositor

   Marius Wernig

   Article

   Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

   Insert

   NeuroD1 (Neurod1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1-FHC

   Plasmid

   #64874

   Purpose

   lentiviral vector with Flag, HA tags

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-puro-cOVA-IRES-BFP

   Plasmid

   #135074

   Purpose

   Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.

   Depositor

   Maria Castro

   Insert

   cOVA-IRES-BFP (OVAL Chicken)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21b(+)-Is-PETase-W159H-S238F

   Plasmid

   #112203

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
   

   Use

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W159H, S238F

   Promoter

   N/A

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CW3SL-EGFP

   Plasmid

   #61463

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. ilux pGEX(-)

   Plasmid

   #107879

   Purpose

   Bacterial expression of ilux for bioluminescence imaging

   Depositor

   Stefan Hell

   Article

   Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

   Insert

   ilux
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

   Plasmid

   #180263

   Purpose

   Lentiviral expression vector for dCas9-VP64 in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-LC3

   Plasmid

   #21073

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   CMV

   Available since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-hAR

   Plasmid

   #89078

   Purpose

   mammalian expression of human androgen receptor in pCMV5

   Depositor

   Elizabeth Wilson

   Article

   Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

   Insert

   human androgen receptor (hAR) full-length (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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