Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 3,985 results for: LYC

Showing: 3201 - 3250 of 3985 results

  1. B-HypaSpCas9

    Plasmid
    #126763
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A; M694A; Q695A; H698A; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126763-plasmid-map-sequence-id-410968

    Enlarge

  2. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

    Enlarge

  4. pDONR223 LC3B G120A no-stop

    Plasmid
    #123206
    Purpose
    Gateway entry clone encoding human MAP1LC3B G120A lacking stop codon, suitable for C-terminal tagging
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Gateway entry vector / entry clone
    Tags
    Expression
    Mutation
    Glycine 120 to Alanine, stop codon removed
    Promoter
    Available since
    June 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    123206-plasmid-map-sequence-id-246646

    Enlarge

  8. pCAGGS-SARS2-S-D614 (C-flag)

    Plasmid
    #156420
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Michael Farzan, Hyeryun Choe
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    156420-plasmid-map-sequence-id-307822

    Enlarge

  9. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid
    #119816
    Purpose
    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
    Depositor
    Ethan Abel
    Article
    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:
    Inserts
    Luciferase
    Puromycin
    Use
    Lentiviral and Luciferase
    Tags
    EGFP and V5
    Expression
    Mammalian
    Mutation
    Stop codon removed in the frame of the V5 frame v…
    Promoter
    CMV/TO and Human Phosphoglycerate kinase
    Available since
    Jan. 2, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119816-plasmid-map-sequence-id-372950

    Enlarge

  10. pVC-FUS-WT

    Plasmid
    #175066
    Purpose
    expresses mVenus and mCherry tagged to FUS wildtype in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175066-plasmid-map-sequence-id-372335

    Enlarge

  11. pFUS-WT-VC

    Plasmid
    #175068
    Purpose
    expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Use
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175068-plasmid-map-sequence-id-353791

    Enlarge

  12. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid
    #145145
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Use
    Tags
    8his tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145145-plasmid-map-sequence-id-324821

    Enlarge

  14. pMG024 MBP-GSK3β-HA-His, GST_λPPase

    Plasmid
    #196182
    Purpose
    Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Use
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196182-plasmid-map-sequence-id-383259

    Enlarge

  15. pCAGGS-FuG-B2

    Plasmid
    #67513
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.
    Insert
    Fusion Glycoprotein type B2
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Expression
    Mutation
    Promoter
    CAGGS
    Available since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67513-plasmid-map-sequence-id-381535

    Enlarge

  17. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid
    #141183
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    Use
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141183-plasmid-map-sequence-id-288058

    Enlarge

  18. pAJE3-E7

    Plasmid
    #214359
    Purpose
    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins
    Depositor
    Ryan Mehl
    Article
    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-
    Inserts
    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    AmpR and lpp
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214359-plasmid-map-sequence-id-424910

    Enlarge

  21. pDRM18 LTN (Luciferase tdTomato Neo)

    Plasmid
    #174721
    Purpose
    Expresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    tdTomato
    aph
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and P2A linker
    Expression
    Mammalian
    Mutation
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174721-plasmid-map-sequence-id-349021

    Enlarge

  23. eMscL G22S

    Plasmid
    #107455
    Purpose
    eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Bearing a glycine to serine substitution at posit…
    Promoter
    human synapsin 1
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    107455-plasmid-map-sequence-id-207549

    Enlarge

  24. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

    Enlarge

  27. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid
    #123235
    Purpose
    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123235-plasmid-map-sequence-id-378779

    Enlarge

  30. MSP1-bio

    Plasmid
    #47709
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP1 (MSP1 Plasmodium falciparum, Synthetic)
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47709-plasmid-map-sequence-id-68315

    Enlarge

  32. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159752-plasmid-map-sequence-id-313824

    Enlarge

  33. A3Ai-Cas9n-UGI-NLS

    Plasmid
    #109425
    Purpose
    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109425-plasmid-map-sequence-id-212947

    Enlarge

  34. pCAGGS-SARS2-S-G614 (C-flag)

    Plasmid
    #156421
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Michael Farzan, Hyeryun Choe
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    156421-plasmid-map-sequence-id-307829

    Enlarge

  39. pAAV-UbC-tdTomato

    Plasmid
    #62516
    Purpose
    Expresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures
    Depositor
    Pantelis Tsoulfas, Jae Lee
    Article
    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/
    Insert
    tdTomato
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hUbC
    Available since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    62516-plasmid-map-sequence-id-107809

    Enlarge

  45. pLenti PGK Puro GFP-LC3B G120A

    Plasmid
    #123242
    Purpose
    Expression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    PGK
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123242-plasmid-map-sequence-id-244541

    Enlarge

  48. pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

    Plasmid
    #164845
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Use
    Tags
    8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression. Has N5…
    Promoter
    CMV
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164845-plasmid-map-sequence-id-322928

    Enlarge

  49. pCAGGS-SARS2-S-D614 (NC-flag)

    Plasmid
    #156418
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Michael Farzan, Hyeryun Choe
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    156418-plasmid-map-sequence-id-307819

    Enlarge

Showing: 3201 - 3250 of 3985 results