Addgene

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CpcB•PHLS+Cpc

Plasmid

#73335

Purpose

Expresses the PHLS gene as a fusion with the CpcB gene followed by the CmR gene

Depositor

Anastasios Melis

Article

Formighieri et al Metab Eng. 2015 Nov;32:116-24. doi: 10.1

Insert

cpcB•PHLS-CmR

Use

Tags

Expression

Mutation

Promoter

cpc operon

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B12-Leum

Plasmid

#44320

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B12-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B12-Leum

Available since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B5-Leum

Plasmid

#44313

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B5-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B5-Leum

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B10-Leum

Plasmid

#44318

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B10-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B10-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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p70rg 5

Plasmid

#17829

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B8-TEF(203)

Plasmid

#44378

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(203) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(203)

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(804)

Plasmid

#44368

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(804) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(804)

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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NarP-GFP

Plasmid

#220161

Purpose

To track the natural NarP promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

NarP promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-PuroR

Plasmid

#232753

Purpose

IVT template for making modRNA encoding the Puromycin resistance gene

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

PuroR

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pC016-gPsp-EGFP_start_codon

Plasmid

#233613

Purpose

Expression of guide RNA targeting the start codon of the EGFP gene for PspCas13b

Depositor

Shintaro Iwasaki

Article

Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

Insert

gRNA targeting EGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G [backbone]

Plasmid

#229156

Purpose

Gene backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-Tn7-Pcym

Plasmid

#225280

Purpose

Mini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.

Depositor

Mark Schembri

Article

Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pcym cumate (p-isopropylbenzoate)-inducible promo…

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-Tn7-PcL

Plasmid

#225281

Purpose

Mini-Tn7 pGP-Tn7-Cm vector with constitutive PcL promoter for the constitutive expression of target genes inserted into the attTn7 chromosomal site.

Depositor

Mark Schembri

Article

Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Constitutive PcL promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-Tn7-PrpsM

Plasmid

#225282

Purpose

Mini-Tn7 pGP-Tn7-Cm vector with constitutive PrpsM promoter for the constitutive expression of target genes inserted into the attTn7 chromosomal site.

Depositor

Mark Schembri

Article

Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Constitutive PrpsM promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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JD134

Plasmid

#229490

Purpose

Adenoviral E4 gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E4

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

wt promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAGK43

Plasmid

#222223

Purpose

Edits NPAS2 Gene.

Depositor

Seth Shipman

Article

Crawford et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

Eco1 RT; 112 nt donor; PAM -19 (NPAS2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-AC17-5c-frGFP

Plasmid

#217525

Purpose

AC17-5c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

Depositor

Keisuke Fukunaga

Article

Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

Insert

AC17-5c riboswitch-regulated folding reporter GFP

Use

In vitro transcription-translation

Tags

Expression

Mutation

Promoter

T7

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-Theo-3c-EGFP

Plasmid

#217526

Purpose

Theo-3c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

Depositor

Keisuke Fukunaga

Article

Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

Insert

Theo-3c riboswitch-regulated EGFP

Use

In vitro transcription-translation

Tags

Expression

Mutation

Promoter

T7

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD_A-AtUBI10-dCas9-24XGP41

Plasmid

#202011

Purpose

Moclo level 1 - Module A, Promoter: AtUBI10, Gene: dCas9-24XGP41, Terminator: HSP

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

Insert

dCas9-24XGP41

Use

Synthetic Biology; Moclo level 1 vector

Tags

Expression

Mutation

Promoter

AtUBI10

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-6MYC osTIR1

Plasmid

#193320

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Use

Tags

6MYC

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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