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pXD70Tet(DadRmt1)-DadR(ΔDBD)

Plasmid

#191637

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔDBD) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Use

Tags

Expression

Bacterial

Mutation

ΔDBD

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadRmt2)-DadR(ΔTM)

Plasmid

#191638

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔTM) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Use

Tags

Expression

Bacterial

Mutation

ΔTM

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadRmt4)-DadR(linker)

Plasmid

#191639

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(linker region shuffled) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Use

Tags

Expression

Bacterial

Mutation

Linker region sequence shuffled

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadH)-Pdadh-DadhABC(A2)

Plasmid

#191644

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-24xopto-TetO-no01

Plasmid

#174887

Purpose

Plasmid vector containing southern blot probe for opto-TetO in STREAMING-tag

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

24xopto-TetO

Use

Southern blot probe

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1

Plasmid

#186709

Purpose

CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Article

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Ptrc.x.tetO2

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TetO(3G)-GCaMP6f-WPRE

Plasmid

#186205

Purpose

Express GCaMP6f under TetO(3G)

Depositor

Naoshige Uchida

Article

Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

Insert

GCaMP6f

Use

AAV

Tags

6xHis (N terminal on insert), T7 epitope, and Xpr…

Expression

Mutation

Promoter

TetO(3G)

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAB300-Tier1-PhCMV-rTetR

Plasmid

#169577

Purpose

Tier-1 vector encoding PhCMV-driven tetracycline-activated rTetR (PhCMV-rTetR-VPR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

reverse tetracycline repressor protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK9c_2

Plasmid

#185023

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK9c_1

Plasmid

#185022

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK10c_2

Plasmid

#185021

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK10c_1

Plasmid

#185020

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_2

Plasmid

#185019

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_1

Plasmid

#185018

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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TetO::EGFP-hMeikin(8A mut)

Plasmid

#174707

Purpose

Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Tags

EGFP

Expression

Mammalian

Mutation

S175A, T176A, T180A, S181A, S196A, T251A, T264A, …

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL

Plasmid

#172200

Purpose

AAV sequence-specific control vector for TALE-Tet1S-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

Depositor

Garrett Kaas

Article

Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

Insert

HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL

Use

AAV and Mouse Targeting; Tale

Tags

HA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

transcription regulatory domain removed

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPD1024 EF1alpha TetON3G in TU5

Plasmid

#139261

Purpose

Expresses TetON3G under hEF1alpha in TUPV5

Depositor

Joshua Leonard

Insert

TetON3G

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hEF1alpha

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJM469 EF1alpha TetON3G in TU4

Plasmid

#139260

Purpose

Expresses TetON3G under hEF1alpha in TUPV4

Depositor

Joshua Leonard

Insert

TetON3G

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hEF1alpha

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJM422 EF1alpha TetON3G in TU3

Plasmid

#139259

Purpose

Expresses TetON3G under hEF1alpha in TUPV3

Depositor

Joshua Leonard

Insert

TetON3G

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hEF1alpha

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA(R1R4)-iRPT

Plasmid

#154882

Purpose

Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

Depositor

Hideyuki Okano

Article

Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

Inserts

iRFP713
Puromycin resistance gene
rtTAM2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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