Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,049 results for: golden gate

Clear keywords
Showing: 1281 - 1300 of 5049 results

  1. pAIP4_4O6G

    Plasmid
    #234175
    Purpose
    Heterologous protein expression of uncharacterized protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4O6G
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234175-plasmid-map-sequence-id-464140

    Enlarge

  2. pAIP4_3K1H

    Plasmid
    #234157
    Purpose
    Heterologous protein expression of O25709_HELPY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3K1H
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234157-plasmid-map-sequence-id-464123

    Enlarge

  3. pBD006

    Plasmid
    #201306
    Purpose
    D16: Promoter of TraesCS5D02G320700 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D16: Promoter of TraesCS5D02G320700
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    201306-plasmid-map-sequence-id-409554

    Enlarge

  4. pBD004

    Plasmid
    #201304
    Purpose
    D14: Promoter of TraesCS3D02G368800 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D14: Promoter of TraesCS3D02G368800
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    201304-plasmid-map-sequence-id-409541

    Enlarge

  5. pBD005

    Plasmid
    #201305
    Purpose
    D15: Promoter of TraesCS3D02G517500 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D15: Promoter of TraesCS3D02G517500
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201305-plasmid-map-sequence-id-409522

    Enlarge

  6. pPD1023 TRE3GV DsRedExpress2 in TU5

    Plasmid
    #139257
    Purpose
    TRE3GV promoter and DsRedExpress2 in TUPV5
    Depositor
    Joshua Leonard
    Insert
    DsRedExpress2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139257-plasmid-map-sequence-id-325370

    Enlarge

  7. C77m

    Plasmid
    #120989
    Purpose
    MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AmpR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120989-plasmid-map-sequence-id-236056

    Enlarge

  8. C78m

    Plasmid
    #120990
    Purpose
    MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ChlorR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120990-plasmid-map-sequence-id-236059

    Enlarge

  9. pAV10.F3

    Plasmid
    #63199
    Purpose
    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63199-plasmid-map-sequence-id-108468

    Enlarge

  10. pAV10.F6.loxP

    Plasmid
    #63201
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    63201-plasmid-map-sequence-id-267056

    Enlarge

  13. pECNUS4_MP

    Plasmid
    #228358
    Purpose
    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228358-plasmid-map-sequence-id-455680

    Enlarge

  15. pAV10.F3.loxP

    Plasmid
    #63200
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63200-plasmid-map-sequence-id-104877

    Enlarge

  16. pAV10.K6.loxP

    Plasmid
    #63203
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63203-plasmid-map-sequence-id-104900

    Enlarge

  17. pAV10.K5.loxP

    Plasmid
    #63204
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63204-plasmid-map-sequence-id-104903

    Enlarge

  18. pAV10.HO3.loxP

    Plasmid
    #63205
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63205-plasmid-map-sequence-id-104905

    Enlarge

  19. pAV10.HO5.loxP

    Plasmid
    #63206
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63206-plasmid-map-sequence-id-104906

    Enlarge

  20. 35S:GOLD (pICSL11383)

    Plasmid
    #252523
    Purpose
    Level 1 plasmid in second position containing 35S:GOLD (pICSL11383)
    Depositor
    Sophien Kamoun
    Insert
    GOLD
    Expression
    Plant
    Promoter
    Short 35s + Ω
    Available Since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    252523-plasmid-map-sequence-id-506599

    Enlarge

Showing: 1281 - 1300 of 5049 results