We narrowed to 1,328 results for: piggyBac

1. epB_CAG_TetRFlag-nls-tdTom_DEx4

   Plasmid

   #119909

   Purpose

   ePiggyBac mammalian expression plasmid for TetR-tdTom fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   TetRFlag-nls-tdTom
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. epB_UbC_tdMS2cp-tagRFP-Tx2

   Plasmid

   #119908

   Purpose

   ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   HA-tdMS2cp-tagRFP-Tx2-nls
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-6HIS-TEV-mEGFP,IRES-NGFR

   Plasmid

   #164521

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-U6-sgRNA-BFP-Puro

   Plasmid

   #229013

   Purpose

   Perturb Seq sgRNA vector with PiggyBac backbone

   Depositor

   Gary Hon

   Article

   Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

   Plasmid

   #197031

   Purpose

   Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized denOsCas12f1-VPR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D52R / T132R / D228A / D406A

   Promoter

   CBh, CMV, hU6

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB-Tet-On-HTR6-RhoA sensor

   Plasmid

   #189614

   Purpose

   Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   HTR6 (HTR6 Human)
   

   Use

   Tags

   GFP and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Tet3G

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218910

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A-huGR/BlastR (NR3C1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 4155C>T and 4729T>G and 4731…

   Promoter

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTR01F/hFGFR3b-V5

   Plasmid

   #236016

   Purpose

   for PiggyBac mediated integration and stable expression of hFGFR3b protein

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   human FGFR3b (FGFR3 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   truncated CMV promoter

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTR01F/hFGFR4-V5

   Plasmid

   #236017

   Purpose

   for PiggyBac mediated integration and stable expression of hFGFR4 protein

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   human FGFR4 (FGFR4 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   truncated CMV promoter

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. TR01F/hFGFR3-G380R-V5

    Plasmid

    #214908

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR3-G380R variant that is associated with Achondroplasia

    Depositor

    Pavel Krejčí

    Article

    Kimura et al Sci Transl Med. 2021 May 5;13(592):eaba4

    Insert

    FGFR3 (FGFR3 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    G380R substitution

    Promoter

    truncated CMV promoter

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #190034

    Purpose

    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A134V,A140V,A141V,A143V

    Promoter

    CBh, SV40, hU6

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

    Plasmid

    #210525

    Purpose

    AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mtagBFP-KASH
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

    Plasmid

    #224435

    Purpose

    AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mScarlet-KASH
    U6-gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR

    Plasmid

    #213481

    Purpose

    AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    GFP-KASH
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #190033

    Purpose

    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13X
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y672A,Y676A

    Promoter

    CBh, SV40, hU6

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #191797

    Purpose

    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized dCas13d
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    R239A, H244A, R858A, H863A

    Promoter

    CBh, SV40, hU6

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-tetO-hNIL

    Plasmid

    #197089

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

    Plasmid

    #213480

    Purpose

    AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mtagBFP-KASH
    U6-gRNA-directcapture
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTR01F/hFGFR2c-V5

    Plasmid

    #236013

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR2c protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR2c (FGFR2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    truncated CMV promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTR01F/hFGFR2b-V5

    Plasmid

    #236014

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR2b protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR2b (FGFR2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    truncated CMV promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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