Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,385 results for: piggyBac

Showing: 1281 - 1300 of 1385 results

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pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)

Plasmid

#242578

Purpose

PiggyBac transposon flanked, Tet-on, gateway destination vector.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-HNRNPK_sgRNA_1

Plasmid

#242897

Purpose

PiggyBac cargo vector with HNRNPK sgRNA 1 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

Insert

HNRNPK (Hnrnpk Mouse)

Use

CRISPR

Promoter

U6

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-HNRNPK_sgRNA_3

Plasmid

#242898

Purpose

PiggyBac cargo vector with HNRNPK sgRNA 3 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

Insert

HNRNPK (Hnrnpk Mouse)

Use

CRISPR

Promoter

U6

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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PB-PE iSM_PPL2

Plasmid

#235996

Purpose

Expresses iSM_PPL2 prime editor and pegRNA in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

iSM_PPL2_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

bpSV40 NLS

Expression

Mammalian

Mutation

replacement of 5 amino acids of Smac-Cas9 PL2 wit…

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPB.DEST-ITGB1(YYFF)-mRuby2

Plasmid

#215449

Purpose

Piggybac expression vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Transposon-based stable expression

Tags

mRuby2

Expression

Mammalian

Mutation

Mutations in the NPxY sites Y783F, Y795F; Silent …

Promoter

CAG

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

Plasmid

#229824

Purpose

A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

Plasmid

#229837

Purpose

A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

Plasmid

#229840

Purpose

A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FIGLA

Plasmid

#222929

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

FIGLA (FIGLA Human)

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HHEX

Plasmid

#222928

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HHEX

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

HHEX (HHEX Human)

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DLX5

Plasmid

#222927

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DLX5

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

DLX5 (DLX5 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-LHX8

Plasmid

#222921

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of LHX8

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

LHX8 (LHX8 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pb-miRE-tre-3xFLAG-hnRNPU∆RGG

Plasmid

#196088

Purpose

Expresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAF-A (Hnrnpu Mouse)

Tags

N-terminal 3xFLAG

Expression

Bacterial and Mammalian

Mutation

removed last 154 amino acids on cterminal end

Promoter

TRE

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-LHX9

Plasmid

#192921

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of LHX9

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

LHX9 (LHX9 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HOXC9

Plasmid

#192910

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of HOXC9

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

HOXC9 (HOXC9 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-LHX1

Plasmid

#192905

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of LHX1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

LHX1 (LHX1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197026

Purpose

Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized enOsCas12f1

Expression

Mammalian

Mutation

D52R / T132R

Promoter

CBh, CMV, hU6

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197027

Purpose

Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized enRhCas12f1

Expression

Mammalian

Mutation

L270R

Promoter

CBh, CMV, hU6

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-GATA4

Plasmid

#192896

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

GATA4 (GATA4 Human)

Tags

HA

Expression

Mammalian

Mutation

Codon-optimized

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RUNX1

Plasmid

#192928

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

RUNX1 (RUNX1 Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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