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pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-p50

Plasmid

#107977

Purpose

Expresssion of NF-kB p50

Depositor

Georg Weber

Insert

NF-kB p50 (Nfkb1 Mouse)

Tags

N/A

Expression

Mammalian

Mutation

Amino acids VQACI added to C-terminus

Promoter

CMV

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218857

Purpose

Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Tags

QF fusion protein

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast

Plasmid

#205555

Purpose

Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV mClover3-Galectin-3

Plasmid

#215376

Purpose

Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mClover3 tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

LGALS3 (LGALS3 Human)

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-mR7BD

Plasmid

#79149

Purpose

bacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)

Depositor

Aimee Edinger

Article

Romero Rosales et al Mol Biol Cell. 2009 Jun;20(12):2831-40.

Insert

R7BD (Rilp Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 220-299

Promoter

tac

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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3X FLAG-FUS-WT

Plasmid

#44985

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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M13KO7-ΔPS-ΔgeneIII

Plasmid

#134351

Purpose

Helper phage for M13 phage production

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Helper phage gene

Expression

Bacterial

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-puro

Plasmid

#45561

Depositor

Michael McVoy

Article

Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

Insert

Puromycin N-acetyl transferase

Use

Bifunctional selection protein

Tags

EGFP

Expression

Mammalian

Promoter

Phcmv (Strong human cytomegalovirus immediate ear…

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

Plasmid

#64512

Purpose

Expression of St1 dCas9-3XTagBFP2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XmTagBFP2

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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