162,717 results

1. pAcGFP-Hyg-CTCF-N1

   Plasmid

   #131799

   Purpose

   Expresses human CTCF-GFP in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6.3/TO/V5-DEST-IGFBP2-Clover

   Plasmid

   #191008

   Purpose

   Lentiviral expression vector for IGFBP2 with a fluorescent Clover tag

   Depositor

   Johanna Ivaska

   Article

   Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

   Insert

   Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
   

   Use

   Lentiviral

   Tags

   Clover

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. HT PTP-RN

   Plasmid

   #24056

   Depositor

   Frederick Stanley

   Insert

   PTPRN (PTPRN Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. Mm-PylRS-AF/Pyl-tRNACUA

   Plasmid

   #122650

   Purpose

   tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   PylRS-AF, Pyl-tRNACUA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

   Promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAGM9121_His2-GFP11

   Plasmid

   #153511

   Purpose

   His2-GFP11 C-terminal Tag Donor Plasmid

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

   Insert

   His2-GFP11
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas-hHDAC3-R265P

   Plasmid

   #103786

   Purpose

   Expresses dCas9 fused to human HDAC3 with R265P mutation

   Depositor

   Zhaolan Zhou

   Article

   Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

   Insert

   dCas9-HDAC3-R265P (HDAC3 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed arginine 265 to proline

   Promoter

   EF1A

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6_(Gluc)_INT(GenPurpClon)

   Plasmid

   #68433

   Purpose

   General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct
   

   Use

   CRISPR; Cloning vector for generating int-like co…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. ND4.2-Right DdCBE-G1397-C- T1413I-GFP

   Plasmid

   #179686

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T1413I

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HSA3_His

   Plasmid

   #202765

   Purpose

   human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   HSA3
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TMED9-bio-His

    Plasmid

    #51855

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein 9 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED9 (TMED9 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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