We narrowed to 9,586 results for: Coli
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Plasmid#139875PurposeExpression plasmid of rings 2 and 3 of LetB for crystallographyDepositorInsertLetB
ExpressionBacterialMutationLetB(159-383)-6xHisAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pClodANL-FGF2NBAA
Plasmid#107873PurposeBacterial expression of FGF2 minimum export mutant-NanoLucDepositorInsertFGF2-minimal export
UseLuciferaseTagsNanoLucExpressionBacterialMutationC77A, C95A, K128Q, R129Q, K134QPromoterpro DAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p005-RFP-weak
Plasmid#108316PurposeSynthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.DepositorInsertRFP under controlf of weak synthetic constitutive promoter pFAB4024
ExpressionBacterialPromoterpFAB4024Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-BBa_J23100
Plasmid#74097PurposeBBa_J23100 promoter (iGEM collection)DepositorInsertBba_J23100 constitutive promoter (Sigma70)
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ppj220(JBEI-13483)
Plasmid#89786Purposefor expression of C-StrepII-3607DepositorInsert3607
TagsStrepIIExpressionBacterialAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK_proNR2_LGK.9
Plasmid#81162PurposeExpresses levoglucosan kinase variant LGK.9 with promoter proNR2DepositorInsertLevoglucosan kinase
TagsHis6xExpressionBacterialMutationLGK.9 - (T15R, A57Q, R94G, R100C, N101G, S105R, L…PromoterproNR2Available SinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
SAH0141-0.0-conP01
Plasmid#79828PurposeTranscriptional interference assessment; strong interfering promoter, landing site for spacersDepositorInsertsBlue fluorescent protein
mCherry
Promoterbla P3 and conP01Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
SAH0146-0.5-conP01
Plasmid#79831PurposeTranscriptional interference assessment; strong interfering promoter, 500 bp spacerDepositorInsertsBlue fluorescent protein
mCherry
Promoterbla P3 and conP01Available SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
SAH0098-scrPX
Plasmid#79830PurposeTranscriptional interference assessment; no interfering promoter (scrambled)DepositorInsertsBlue fluorescent protein
mCherry
Promoterbla P3 and scrPX (no promoter activity)Available SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
MetNI (E166Q in MetN)
Plasmid#78320PurposeMethionine ABC TransporterDepositorInsertsMetI
MetN
Tags10x Histidine Tag and 6x Histidine TagExpressionBacterialMutationE166QPromoterT7 PromoterAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL6
Plasmid#72990PurposeRBS_M3DepositorInsertM3 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL7
Plasmid#72991PurposeRBS_M5DepositorInsertM5 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL9
Plasmid#72993PurposeRBS_L2DepositorInsertL2 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL10
Plasmid#72994PurposeRBS_L3DepositorInsertL3 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL12
Plasmid#72996PurposeRBS_L5DepositorInsertL5 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L3S2P21
Plasmid#72999PurposeL3S2P21 terminatorDepositorInsertL3S2P21 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L3S1P51
Plasmid#73000PurposeL3S1P51 terminatorDepositorInsertL3S1P51 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L3S1P32
Plasmid#73001PurposeL3S1P32 terminatorDepositorInsertL3S1P32 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L3S1P11
Plasmid#73002PurposeL3S1P11 terminatorDepositorInsertL3S1P11 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L2U8H11
Plasmid#73003PurposeL2U8H11 terminatorDepositorInsertL2U8H11 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L2U5H11
Plasmid#73004PurposeL2U5H11 terminatorDepositorInsertL2U5H11 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L2U5H08
Plasmid#73005PurposeL2U5H08 terminatorDepositorInsertL2U5H08 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L2U3H03
Plasmid#73006PurposeL2U3H03 terminatorDepositorInsertL2U3H03 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L2U2H09
Plasmid#73007PurposeL2U2H09 terminatorDepositorInsertL2U2H09 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L1U1H08
Plasmid#73009PurposeL1U1H08 terminatorDepositorInsertL1U1H08 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL1
Plasmid#72985PurposeRBS_H1DepositorInsertH1 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL2
Plasmid#72986PurposeRBS_H2DepositorInsertH2 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL3
Plasmid#72987PurposeRBS_H3DepositorInsertH3 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVD432
Plasmid#63755PurposeProbe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.DepositorInsertEAggEC
ExpressionBacterialAvailable SinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD402
Plasmid#66148Purposeto make a probe to detect Heat stable enterotoxin gene (human)DepositorInsertSTh
UseCloning vectorPromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD404
Plasmid#66149Purposeto make a probe to detect Heat stable enterotoxin gene (porcine)DepositorInsertSTp
UseCloning vectorPromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1318
Plasmid#37637DepositorInsertsTetR
CsrA
UseSynthetic BiologyExpressionBacterialPromoterpConNoHindAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pDL11c
Plasmid#17833DepositorInsertalcA promoter, lacZ, argB_1626,
UseSynthetic Biology; Aspergillus nidulans expressionMutationThe argB_1626 mutation consists of two sequential…Available SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKB235.4
Plasmid#170026PurposeEncodes LacI-controlled expression of surface-displayed CCL20 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL20 Δ alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailabilityAcademic Institutions and Nonprofits only -
pKB236.1
Plasmid#170028PurposeEncodes LacI-controlled expression of surface-displayed TCP fragment lacking C-terminal alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
TCP Δ C-terminal alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailabilityAcademic Institutions and Nonprofits only -
pKB236.2
Plasmid#170029PurposeEncodes LacI-controlled expression of surface-displayed TCP C-terminal alpha-helix fragment and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
TCP C-terminal alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailabilityAcademic Institutions and Nonprofits only -
pARC8-LambdaRedBeta-EcSSB-EcMutLE32K
Plasmid#162576PurposeArabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.DepositorInsertsLambda Red-Beta
E. coli SSB
EcMutLE32K
ExpressionBacterialMutationE32K mutated to give dominant negative phenotypePromoterpBADAvailable SinceMay 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS_V65I
Plasmid#98567PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
Mutated ATP-dependent Clp protease adapter protein
ExpressionBacterialMutationContains the V65I mutation that increases discrim…Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJ1
Plasmid#138379PurposepEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZDepositorTagsHis10ExpressionBacterialMutationClamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…PromoterT7Available SinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL1del
Plasmid#216749PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
Tags10x HisExpressionBacterialMutationOuter loop 1 (OL1) with the sequence of [FKISLAEM…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL2del
Plasmid#216750PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
Tags10x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [WMGILKNN…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL3del
Plasmid#216751PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 3 (OL3) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
Tags10x HisExpressionBacterialMutationOuter loop 3 (OL3) with the sequence of [ELLGGPDS…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL3/2sub
Plasmid#216752PurposeStudy the interaction of PotH in E. coli, specifically focusing on the variant in which outer loop 2 (OL2) is substituted with OL3 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
Tags10x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [WMGILKNN…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM253 (miniCTX1-rhaSR-PrhaBAD)
Plasmid#110571PurposeIntegration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
ExpressionBacterialPromoterrhaSR-PrhaBADAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Flp
Plasmid#122969PurposeExpresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.DepositorInsertFlp recombinase
ExpressionBacterialPromoterpBADAvailable SinceAug. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTXB1-Tn5
Plasmid#60240PurposeIPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.DepositorInserttnpA from pBam1
TagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7 promoterAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMS2
Plasmid#191123PurposeTranscription of 12xMS2 repeats from a tetracycline-inducible promoterDepositorInsertMS2
UseSynthetic BiologyPromotertetracycline-inducible promoterAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
secreted BirA-Flag
Plasmid#64395PurposeFlag-tagged biotinylation enzyme for co-expression with bio-proteinsDepositorAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-Streptavidin-K121R
Plasmid#89880PurposeStreptavidin that has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K121R-Glutamate_Tag
Tags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK121RAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only