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Plasmid#157900PurposeExpresses the dsr1 defense systemDepositorInsertdsr1 (SIR2-DUF4020)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAJM.690
Plasmid#108533PurposePcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.DepositorInsertPcaUAM + P3B5B-YFP
UseTagsExpressionBacterialMutationPcaU: K14N A165T N201HPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRT802
Plasmid#68113PurposeIntegration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
aph11
oriT
attP
int
UseStreptomyces integrating vectorTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLG016 (ApeA)
Plasmid#157894PurposeExpresses the ApeA defense systemDepositorInsertApeA (putative HEPN RNase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
MazF-WT
Plasmid#155202PurposeTo express recombinant MazF protein.DepositorInsertMazF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF280
Plasmid#167675PurposeIntermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBI (JBEI-3085)
Plasmid#87950Purposethe overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).DepositorInsertMevT-MBI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAJM.683
Plasmid#108531PurposeBetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.DepositorInsertBetIAM + PBetI-YFP
UseTagsExpressionBacterialMutationBetI: S115IPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
ABE8r-V106W
Plasmid#208295PurposeExpresses ABE8r in mammalian cellsDepositorInsertTadA8r-V106W-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM901
Plasmid#72966PurposeLevel 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM901 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS sfGFP
Plasmid#154462PurposepET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKB083
Plasmid#181952PurposeIPTG-inducible expression of PhoQDepositorInsertPhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
UseTagsExpressionBacterialMutationPromoterPhoQ-Ptac; lacI-PlacIqAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGD236
Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2SK
Plasmid#59383PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.DepositorInsertTT-ISceI-kan
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSF278
Plasmid#167673PurposeIntermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
ABE8r-R153del
Plasmid#208296PurposeExpresses ABE8r in mammalian cellsDepositorInsertTadA8r-R153del-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2SC
Plasmid#59382PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKL002
Plasmid#98921PurposepET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.DepositorInsertsPROPS
GCaMP6f
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBIS
Plasmid#35150DepositorInsertAtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLacUV5,Available sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-rfp
Plasmid#153038PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)
Plasmid#110554PurposeminiTn7 delivery plasmid with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
UseTagsExpressionBacterialMutationPromoterAraC-ParaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM251 (miniCTX1-araC-ParaBAD)
Plasmid#110567PurposeIntegration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
UseTagsExpressionBacterialMutationPromoteraraC-ParaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRF0G-Tyr
Plasmid#113644PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.DepositorInsertMjYRS and tRNA gene
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMD1 anti-GFP nanobody-Tn5
Plasmid#215567PurposeExpresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tagDepositorInsertanti-GFP nanobody-Tn5 fusion
UseTags3xFlag and intein-CBDExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
prCAG-BoxB
Plasmid#191122PurposeTranscription of 47xCAG and 12xBoxB repeats from a tetracycline-inducible promoterDepositorInsertsrCAG
BoxB
UseSynthetic BiologyTagsExpressionMutationPromotertetracycline-inducible promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsaI
Plasmid#128649PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAJM.969
Plasmid#108537PurposeMphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.DepositorInsertMphRAM + EryR + PMph-YFP
UseTagsExpressionBacterialMutationMphR: N94DPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LplA(W37V) Coumarin PRIME ligase
Plasmid#25845DepositorInsertLplA-W37V
UseTagsFlag and HisExpressionMammalianMutationW37VPromoterAvailable sinceJuly 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII12Pglu-700uDelta6
Plasmid#13568DepositorInsertFLIPglu F16A Y12S-ECFP-D13R, 6aa linker
UseTagsCitrineExpressionBacterialMutationF16A, ECFP insert in mglBPromoterAvailable sinceFeb. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pXint129
Plasmid#178208PurposeEncodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.DepositorUseTagsExpressionBacterialMutationPromoterlacIAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMYO127TAG-SepT
Plasmid#34625DepositorInsertMyoglobin-127TAG
UseTags6xHISExpressionBacterialMutationcodon-optimized + an amber stop codon was introdu…PromoterIppAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)
Plasmid#70198PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
RSV-betaGal
Plasmid#24058DepositorInsertLacZ (lacZ E. coli)
UseTagsRSV promoterExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET15b T7pCONS sfGFP
Plasmid#154467PurposepET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST5832
Plasmid#36256DepositorInsertlacZ
UseSynthetic Biology; Mycobacteria replicating vectorTagsExpressionBacterialMutationPromoterModified Hsp60Available sinceNov. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMS2
Plasmid#191123PurposeTranscription of 12xMS2 repeats from a tetracycline-inducible promoterDepositorInsertMS2
UseSynthetic BiologyTagsExpressionMutationPromotertetracycline-inducible promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-I
Plasmid#53437Purposexylose-dependent expression of the type-I agrC and agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-INSyibp-Nef
Plasmid#134593PurposeLow Phosphate inducible NefDepositorInsertHIV Nef protein
UseTagsHis tagExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRT801
Plasmid#68112PurposeIntegration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
oriT
attP
int
UseStreptomyces integrating vectorTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-SIN6.1 CBLacZ-LucWm1A
Plasmid#44167DepositorInsertsLacZ
firefly luciferase
UseLentiviral and LuciferaseTagsExpressionMutationPromoterCB, CMV enhancer/chicken beta-actin promoterAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21-Streptavidin-K121R
Plasmid#89880PurposeStreptavidin that has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K121R-Glutamate_Tag
UseTags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK121RPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCO706
Plasmid#139743PurposeIndividual component of Caffeic Acid biosynthesis pathway, hpaCDepositorInserthpaB
UseMoclo level 0 componentTagsExpressionMutationPromoternoneAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCO707
Plasmid#139744PurposeIndividual component of Caffeic Acid biosynthesis pathway, hpaCDepositorInserthpaC
UseMoclo level 0 componentTagsExpressionMutationPromoternoneAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEINO5
Plasmid#179489PurposeMulti-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugationDepositorInsertaacC1
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF279
Plasmid#167674PurposeIntermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDrop
Plasmid#184872PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOxyR_gfp
Plasmid#194154PurposeGreen Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
UseTagsExpressionBacterialMutationPromoterPoxyR, PoxySAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only