We narrowed to 9,455 results for: Coli

1. pTXB1-Tn5

   Plasmid

   #60240

   Purpose

   IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

   Depositor

   Rickard Sandberg

   Article

   Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

   Insert

   tnpA from pBam1
   

   Tags

   Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   T7 promoter

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas

   Plasmid

   #62225

   Purpose

   Constitutive expression of cas9 and inducible expression of lambda RED and sgR

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Has Service

   Cloning Grade DNA

   Insert

   cas9
   

   Use

   CRISPR; Lambda red recombinase

   Expression

   Bacterial

   Promoter

   native cas9 promoter

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJUMP24_T24_pRham-ilvA-relE

   Plasmid

   #201531

   Purpose

   Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Dan Park

   Article

   Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

   Insert

   ilvA-relE
   

   Use

   Synthetic Biology

   Promoter

   PrhaBAD (rhamnose)

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-Flp

   Plasmid

   #122969

   Purpose

   Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

   Depositor

   Lydia Freddolino

   Article

   Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

   Insert

   Flp recombinase
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Aug. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pOxyR_gfp

   Plasmid

   #194154

   Purpose

   Green Fluorescent Reporter of intracellular hydrogen peroxide

   Depositor

   Sven Panke

   Article

   Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

   Insert

   transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
   

   Expression

   Bacterial

   Promoter

   PoxyR, PoxyS

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21a-MetRS-His

   Plasmid

   #124115

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Methionine--tRNA ligase (metG Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

   Plasmid

   #110558

   Purpose

   miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   lacIq-Ptac
   

   Expression

   Bacterial

   Promoter

   lacIq-Ptac

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. secreted BirA-Flag

   Plasmid

   #64395

   Purpose

   Flag-tagged biotinylation enzyme for co-expression with bio-proteins

   Depositor

   Gavin Wright

   Article

   Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

   Insert

   BirA (birA E. coli)
   

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLG006 (Ec67 retron)

   Plasmid

   #157884

   Purpose

   Expresses the Ec67 retron defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   Ec67 retron
   

   Expression

   Bacterial

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pP3-GFP

    Plasmid

    #53436

    Purpose

    Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    P3 promoter
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    P3

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLG007 (Ec86 retron)

    Plasmid

    #157885

    Purpose

    Expresses the Ec86 retron defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    Ec86 retron
    

    Expression

    Bacterial

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLG013 (DRT type 5)

    Plasmid

    #157891

    Purpose

    Expresses the DRT type 5 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 5
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLG004 (RT-Abi-P2)

    Plasmid

    #157882

    Purpose

    Expresses the RT-Abi-P2 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    RT-Abi-P2
    

    Expression

    Bacterial

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSF278

    Plasmid

    #167673

    Purpose

    Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-EF1a-MinD-IRES-Puro

    Plasmid

    #213114

    Purpose

    Expression of untagged MinD in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBES1-LacOx50-mCh-bla

    Plasmid

    #210260

    Purpose

    Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    mCherry
    

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCx55.5-TrpR

    Plasmid

    #86373

    Purpose

    for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    TrpR
    

    Use

    Zebrafish

    Promoter

    cx55.5

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRT801

    Plasmid

    #68112

    Purpose

    Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

    Depositor

    Maggie Smith

    Article

    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

    Inserts

    aac3(IV)
    oriT
    attP
    int
    

    Use

    Streptomyces integrating vector

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p201N Cas9

    Plasmid

    #59175

    Purpose

    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    nptII
    

    Use

    CRISPR

    Promoter

    2x35S and StUbi-3P

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SepOTSλ

    Plasmid

    #68292

    Purpose

    Phosphoserine orthogonal translation system (preferred variant)

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EFSep21, 4xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKL002

    Plasmid

    #98921

    Purpose

    pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Inserts

    PROPS
    GCaMP6f
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHCKan-yibDp-mCherry

    Plasmid

    #127058

    Purpose

    Low phosphate inducible mCherry with N-terminal linker and His tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCold CL7-Cas9

    Plasmid

    #124890

    Purpose

    obtaining full Cas9 RNP

    Depositor

    Yi Liu

    Article

    Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

    Insert

    Cas9
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT2SC

    Plasmid

    #59382

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat
    

    Use

    Template

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21a-LysRS-His

    Plasmid

    #124114

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Lysine-tRNA ligase (lysU Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQE30-His-AlaRS

    Plasmid

    #124103

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Alanyl-tRNA ligase (alaS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BtARR3 short (1-392)

    Plasmid

    #236272

    Purpose

    Bacterial expression of bovine arrestin-3 short (1-392) L393Stop

    Depositor

    Vsevolod Gurevich

    Insert

    arrestin-3 (ARR3 Bovine)
    

    Expression

    Bacterial

    Mutation

    short, (1-392) L393Stop

    Promoter

    lac

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21a-AspRS-His

    Plasmid

    #124106

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Aspartate-tRNA ligase (aspS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET21a-ProRS-His

    Plasmid

    #124117

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Prolyl-tRNA ligase (proS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOxyR_rfp

    Plasmid

    #194155

    Purpose

    Red Fluorescent Reporter of intracellular hydrogen peroxide

    Depositor

    Sven Panke

    Article

    Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

    Insert

    transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
    

    Expression

    Bacterial

    Promoter

    PoxyR, PoxyS

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21a-TyrRS-His

    Plasmid

    #124121

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tyrosyl-tRNA ligase (tyrS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET21a-ValRS-His

    Plasmid

    #124122

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Valyl-tRNA ligase (valS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a T7pCONS sfGFP

    Plasmid

    #154462

    Purpose

    pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Tags

    His-thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ABE8r-V106W

    Plasmid

    #208295

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-V106W-SpCas9 D10A
    

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)

    Plasmid

    #110554

    Purpose

    miniTn7 delivery plasmid with araC-ParaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    araC-ParaBAD
    

    Expression

    Bacterial

    Promoter

    AraC-ParaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBXNH3CA_MBP

    Plasmid

    #132700

    Purpose

    expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Maltose binding Protein
    

    Tags

    Avi and His

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV-EF1a-MinE-EGFP_IRES-Blast

    Plasmid

    #213113

    Purpose

    Expression of MinE-EGFP in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21a-GluRS-His

    Plasmid

    #124109

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutamyl-tRNA ligase (gltX Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAJM.336

    Plasmid

    #108528

    Purpose

    LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    LacIAM + PTac-YFP
    

    Expression

    Bacterial

    Mutation

    LacI Q18M A47V F161Y

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET21a-SerRS-His

    Plasmid

    #124118

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Seryl-tRNA ligase (serS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET21a-GlnRS-His

    Plasmid

    #124108

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutaminyl-tRNA ligase (glnS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pQE60-EFTu-His

    Plasmid

    #124127

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor Tu (tufB Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET16b-His-ArgRS

    Plasmid

    #124104

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Argin+I4 ligase (argS Escherichia coli)
    

    Tags

    10x-His

    Expression

    Bacterial

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pQE30-His-AsnRS

    Plasmid

    #124105

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Asparaginyl-tRNA ligase (asnS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pQE60-EFG-His

    Plasmid

    #124126

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor G (fusA Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAJM.661

    Plasmid

    #108532

    Purpose

    TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    TtgRAM + PTtg-YFP
    

    Expression

    Bacterial

    Mutation

    TtgR: R130H A144T A148V

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pQE30-His-ThrRS

    Plasmid

    #124119

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Threonyl-tRNA ligase (thrS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRF0G-Tyr

    Plasmid

    #113644

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    MjYRS and tRNA gene
    

    Use

    Synthetic Biology

    Available Since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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50. pET21a-His-IleRS

    Plasmid

    #124112

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Isoleucyl-tRNA ligase (ileS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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