Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,797 results for: plasmid dna

Showing: 3251 - 3300 of 5797 results

  1. NanoBRET Assay Vector, NanoLuc-KRAS(G12V)

    Plasmid
    #236859
    Purpose
    Express NanoLuc(R)-KRAS(G12V) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    CMV
    Available Since
    Sept. 22, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236859-plasmid-map-sequence-id-486819

    Enlarge

  2. NanoBiT EGFR(1-673) Assay, BiBiT vector

    Plasmid
    #238756
    Purpose
    Express EGFR(1-673) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    EGFR (EGFR Human)
    EGFR (EGFR Human)
    Use
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    (1-673)
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238756-plasmid-map-sequence-id-479899

    Enlarge

  3. NanoBRET Assay Vector, BiBRET CRAF-BRAF

    Plasmid
    #238574
    Purpose
    Express CRAF-BRAF Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    CRAF (RAF1 Human)
    BRAF (BRAF Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238574-plasmid-map-sequence-id-479884

    Enlarge

  4. NanoBRET Assay Vector, NanoLuc-KRAS WT

    Plasmid
    #236878
    Purpose
    Express NanoLuc(R)-KRAS WT in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236878-plasmid-map-sequence-id-486139

    Enlarge

  5. LgBiT-CALM1/SmBiT-ADCY8 BiBiT Vector

    Plasmid
    #237114
    Purpose
    Express LgBiT-CALM1/SmBiT-ADCY8 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    CALM1 (CALM1 Human)
    ADCY8 (ADCY8 Human)
    Use
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237114-plasmid-map-sequence-id-474695

    Enlarge

  6. LgBiT-CYBC1:CYBB-SmBiT BiBiT Vector(CMV)

    Plasmid
    #237084
    Purpose
    Express LgBiT-CYBC1:CYBB-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    CYBC1 (CYBC1 Human)
    CYBB (CYBB Human)
    Use
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237084-plasmid-map-sequence-id-473313

    Enlarge

  7. NanoBRET BiBRET Vector MAVS/IFIH1

    Plasmid
    #237022
    Purpose
    Express HaloTag-MAVS/NanoLuc(R)-IFIH1 Fusion Proteins in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    MAVS (MAVS Human)
    IFIH1 (IFIH1 Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237022-plasmid-map-sequence-id-471884

    Enlarge

  15. SunTagng22aa

    Plasmid
    #106438
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106438-plasmid-map-sequence-id-309504

    Enlarge

  18. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid
    #160736
    Purpose
    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    160736-plasmid-map-sequence-id-326992

    Enlarge

  19. hACE2

    Plasmid
    #1786
    Purpose
    Mammalian expression of human ACE2
    Depositor
    Hyeryun Choe
    Article
    Li et al Nature. 2003 Nov 27. 426(6965):450-4.
    Has Service
    Cloning Grade DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    C9 (GTETSQVAPA)
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 14, 2006
    Availability
    Industry, Academic Institutions, and Nonprofits
    1786-plasmid-map-sequence-id-275247

    Enlarge

  22. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid
    #167288
    Purpose
    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.
    Depositor
    Agnel Sfeir
    Article
    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:
    Insert
    DDX58 (RIGI Human)
    Use
    Lentiviral
    Tags
    APEX2 and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    167288-plasmid-map-sequence-id-330940

    Enlarge

  29. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid
    #60365
    Purpose
    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI
    Use
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-tet
    Available Since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60365-plasmid-map-sequence-id-256737

    Enlarge

  30. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid
    #181743
    Purpose
    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181743-plasmid-map-sequence-id-354675

    Enlarge

  31. Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

    Plasmid
    #229795
    Purpose
    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
    Use
    Synthetic Biology
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    TRE and CAG
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229795-plasmid-map-sequence-id-461775

    Enlarge

  33. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid
    #135955
    Purpose
    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Article
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) N-terminal
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135955-plasmid-map-sequence-id-269512

    Enlarge

  36. SunTagng14aa

    Plasmid
    #106439
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106439-plasmid-map-sequence-id-205526

    Enlarge

  37. SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid
    #106435
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    106435-plasmid-map-sequence-id-422469

    Enlarge

  38. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181745-plasmid-map-sequence-id-354676

    Enlarge

  41. pCDH-EF1-FHC-POLQ-K121M

    Plasmid
    #64877
    Purpose
    lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)
    Depositor
    Richard Wood
    Article
    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.
    Insert
    POLQ (POLQ Human)
    Use
    Lentiviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    K121M, a mutation in the conserved ATP-binding si…
    Promoter
    EF1alpha
    Available Since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    64877-plasmid-map-sequence-id-163756

    Enlarge

  43. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    131300-plasmid-map-sequence-id-254651

    Enlarge

  46. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177345
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Synapsin
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177345-plasmid-map-sequence-id-353619

    Enlarge

  49. pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)

    Plasmid
    #68218
    Purpose
    Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Luc / Renilla / P19
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos / 35S / 35S
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68218-plasmid-map-sequence-id-135582

    Enlarge

Showing: 3251 - 3300 of 5797 results