We narrowed to 51,027 results for: des.1

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pVY-1 sfGFP-tag1

Plasmid

#194616

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

sfGFP-KGGSKKRKKR

Tags

KGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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SPELL-GDI Component 1

Plasmid

#170486

Purpose

SPELL GDI1 N-lobe fused to iFKBP. Split site is 66.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

GDI1(N-lobe)-iFKBP

Expression

Mammalian

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shMTH1-2

Plasmid

#21298

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

shMTH1-2 (NUDT1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
SPELL-Lyn Component 1

Plasmid

#170484

Purpose

SPELL Lyn kinase (Constitutively active) N-lobe fused to iFKBP. Split site is 279.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

Lyn(N-lobe), iFKBP

Expression

Mammalian

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (1-100)

Plasmid

#99660

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated p65 activation domain (1-100)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (1-200)

Plasmid

#99658

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated p65 activation domain (1-200)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (1-150)

Plasmid

#99659

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated p65 activation domain (1-150)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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Cbrg-vab-1-RNAi

Plasmid

#175249

Purpose

RNAi feeding bacteria/plasmid to knock-down vab-1 in C. briggsae

Depositor

Anne Brunet

Article

Booth et al Elife. 2019 Jul 8;8. pii: 46418. doi: 10

Insert

vab-1

Use

RNAi

Promoter

T7

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4-sfGFP(1-157)

Plasmid

#220743

Purpose

GAL4-sfGFP(1-157) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-sfGFP(1-157)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET19b NFRKB 1-101

Plasmid

#19787

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Tags

His

Expression

Bacterial

Mutation

only contains amino acids 1-101

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pCGN-HCF-1 fl

Plasmid

#53309

Purpose

plasmid contains human HCFC1 full length gene with HA and c-myc tags

Depositor

Winship Herr

Article

Wilson et al Cell. 1993 Jul 16;74(1):115-25.

Insert

HCFC1 (HCFC1 Human)

Tags

HA and c-myc

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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JARID2-KO-gRNA-1

Plasmid

#131328

Purpose

gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

JARID2-KO-gRNA-1

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
pIS1 IMP-1 rc

Plasmid

#21643

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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EED-KO-gRNA-1

Plasmid

#131321

Purpose

gRNA to knockout EED. Use with EED-KO-gRNA-2

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-1

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
ALIX(1-702)-mNeonGreen

Plasmid

#191193

Purpose

Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (1-702) (PDCD6IP Synthetic, Human)

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Deletion of ALIX C-terminal PRD.

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RIAM(1-666)

Plasmid

#80028

Purpose

Mammalian expression of GFP-RIAM(1-666)

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

RIAM (APBB1IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-666

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
pDECKO-mCherry MALAT1_Enhancer.1

Plasmid

#78536

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.1

Plasmid

#78540

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 D-1

Plasmid

#184013

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 685-706.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-1 (Prom1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of amino acids 685-706.

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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