We narrowed to 5,538 results for: pcas

1. pX330_rSyn1 sgRNA / hSpCas9

   Plasmid

   #172840

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-BsCas12b-2AeGFP

   Plasmid

   #121948

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   BsCas12b
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-HDAC4 C-terminus

   Plasmid

   #71400

   Purpose

   CAG promoter, mammalian expression

   Depositor

   Bo Chen

   Article

   Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

   Insert

   HDAC4 C-terminus (HDAC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCAGGS-S-501V2-FLAG

   Plasmid

   #177096

   Purpose

   501V2 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein 501V2 (S SARS-CoV-2)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pCAGGS-hCD4-GCaMP2

   Plasmid

   #18932

   Depositor

   Karel Svoboda

   Article

   Mao et al PLoS ONE. 2008 . 3(3):e1796.

   Insert

   hCD4-GCaMP2 (CD4 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

6. BII-ChBtW-eSpCas9

   Plasmid

   #133370

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG/hArf4(WT)-EGFP

   Plasmid

   #79407

   Purpose

   Expresses C-terminally EGFP-tagged Arf4(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

   Insert

   Arf4 (ARF4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pGL3-B-838;+80pCalb2

   Plasmid

   #66750

   Purpose

   luciferase reporter for Calb2 promoter (-838 to +80, WT)

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-838bp +80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Calb2

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-S-B.1.617.1-FLAG

   Plasmid

   #177098

   Purpose

   B.1.617.1 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein B.1.617.1 (S SARS-CoV-2)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

    Plasmid

    #107731

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

    Plasmid

    #107728

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFREE2-eSpCas9-sgRNA

    Plasmid

    #179580

    Purpose

    Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    eSpCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-HDAC4 1-747

    Plasmid

    #71399

    Purpose

    CAG promoter, mammalian expression

    Depositor

    Bo Chen

    Article

    Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

    Insert

    HDAC4 1-747 (HDAC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pACYC-OspCas12c-noncoding

    Plasmid

    #120878

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-OspCas12
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT225_(pCA-tdT3Myc)

    Plasmid

    #36873

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tdTomato-3Myc
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-SRGAP3FBAR-EGFP

    Plasmid

    #168506

    Purpose

    Expression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusion

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP3-FBAR (SRGAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-492

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330_CALR sgRNA / hSpCas9

    Plasmid

    #172838

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-AmCas12b-2AeGFP

    Plasmid

    #121947

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AmCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSpCas9(BB)-2A-Intron4-PAFAH1B1

    Plasmid

    #92434

    Purpose

    expresses Cas9 and gRNA targeting PAFAH1B1

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-TetR-RNF169UBD

    Plasmid

    #119011

    Purpose

    Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    TetR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge