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qTAG-N-Puro-sTagRFP-TUBA1B

Plasmid

#207769

Purpose

Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBABR IRFP-Bcd-LEXY

Plasmid

#182595

Purpose

Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Use

Tags

IRFP and LEXY

Expression

Insect

Mutation

Promoter

maternal tubulin

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSF11-wemiRFP670-T2A-HO1

Plasmid

#197243

Purpose

Expresses the protein of wemiRFP670-T2A-HO1 in neurons of C elegan

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wemiRFP670-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

Plasmid

#209727

Purpose

Expresses cytosolic medium affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95K.miRFP670nano3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn1-eNpHR3.0-iRFP713

Plasmid

#197594

Purpose

Lentiviral transfer vector for the neuronal expression of eNpHR3.0-iRFP713 (halorhodopsin for the inhibition of neuronal activity)

Depositor

Patrik Verstreken

Insert

eNpHR3.0-iRFP713

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hSynI

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tsapphire-StagRFP-SrcAR

Plasmid

#173018

Purpose

Long-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

Tsapphire-StagRFP-SrcAR

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCI Cas9 H2B-RFP

Plasmid

#92397

Purpose

CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Violet

Plasmid

#160447

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Violet

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP680-HO1

Plasmid

#197253

Purpose

Expresses the protein of miRFP680 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSAD-5PSD95-EGFP-SynPhRFP

Plasmid

#217979

Purpose

G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

Depositor

Edward Callaway

Article

Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

Inserts

5PSD95-EGFP (Dlg4 Rat)
SynPhRFP (Syp Mouse)

Use

G-deleted rabies

Tags

EGFP and RFP

Expression

Mutation

Promoter

None

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIbleo-iRFP670-P2A-EGFP

Plasmid

#198060

Purpose

The lentiviral vector for iRFP670 and EGFP

Depositor

Michiyuki Matsuda

Insert

iRFP670

Use

Lentiviral

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a-ABCD3-RFP-Puro

Plasmid

#170168

Purpose

Mammalian Expression of Ef1a-ABCD3-RFP-Puro

Depositor

Christopher Moxham

Insert

ABCD3-RFP-Puro

Use

Tags

Expression

Mammalian

Mutation

n/a

Promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-sfGFP-3xP3-RFP

Plasmid

#127566

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sfGFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

July 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP720-HO1

Plasmid

#197257

Purpose

Expresses the protein of miRFP720 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP720

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-RanWT-mRFP1-polyA

Plasmid

#59750

Purpose

Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran-mRFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pKSR1-CA3-mRFP1 (C-KSR)

Plasmid

#217754

Purpose

Fluorescent reporter for ceramide (mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400 plus vector-based linker LE…

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTag-RFP-C-h-Rab7L1-c-Myc

Plasmid

#79804

Purpose

Express TagRFP labeled human Rab7L1.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab7L1 (RAB29 Human)

Use

Tags

TagRFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pPOTv6-puro-3Ty:tagRFPt:3Ty

Plasmid

#201089

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-CAAX-SV40

Plasmid

#117809

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMMP-BiP-IRES-mRFP

Plasmid

#36975

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Insert

BiP (HSPA5 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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