-
Plasmid#65717PurposemNanog reporter plasmid (expresses YNL)DepositorInsertmurine Nanog promoter and YNL
UseLentiviral and LuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 tet(A)
Plasmid#112887PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInserttet(A)
UseTags6xHisExpressionBacterialMutationSequence was codon optimized for E. coli K12 usin…PromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYSD153
Plasmid#212929PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD141
Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-H2B-mRuby3-IRES-eGtACR1-ST
Plasmid#108960PurposeAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequenceDepositorInserteGtACR1-ST
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSR02
Plasmid#69149PurposeRead-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IVDepositorInsertsmCherry
eGFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterrps-27Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXNgate21-3HA
Plasmid#105078PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneUseTagsNubGExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-GST-eIF4G1-His6, eIF4E
Plasmid#37232DepositorUseTagsGST and His6ExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMOX-mNG::LacI-V3
Plasmid#210224PurposeIntegrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.DepositorInsertmNeongreen Lac repressor fusion protein
UseTrypanosoma brucei expressionTagsExpressionMutationPromoterPol II readthrough transcription after integrationAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75S)
Plasmid#137828PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75S)
UseTags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Serine in NTEVp fragmentPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CytERM-mTurquoise2
Plasmid#98833PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-Smad2 EPSM
Plasmid#14933DepositorInsertSmad2 EPSM (SMAD2 Human)
UseTagsFlagExpressionMammalianMutationErk/Pro-directed kinase site mutant (EPSM). T220V…PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TetR-NLS-VP16
Plasmid#103834PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arraysDepositorInsertEGFP-TetR-NLS-VP16 (tetR )
UseTagsExpressionMammalianMutationPromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-jRCaMP1b
Plasmid#191468PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent proteinDepositorInsertjRCaMP1b
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
RT3GEP
Plasmid#111164PurposeTet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsExpressionMutationWTPromoterT3GAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Twitch-2C pcDNA3
Plasmid#104501Purposefluorescent reporter for calcium signalingDepositorInsertTwitch-2C
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mNeptune2
Plasmid#104012PurposePlasmid encoding mNeptune2DepositorInsertmNeptune2
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (MSKGEE LIKENM… R32…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-tdTEVDB_C-15A/G4C
Plasmid#177205PurposePermuted td intron with evolved initiation sequence for the expression of circular mRNADepositorInsertPermuted GFP with evolved initiation sequence for circular mRNA
UseTags6x HisExpressionBacterialMutationPermuted sfGFP after residue 52 & Changed Cyt…PromoteraraBADAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_TUBB5
Plasmid#65678PurposeExpresses beta-tubulin-YNL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
RT3REN
Plasmid#111166PurposeTet-regulated miR-E (miR-30 variant)-based RNAi with DsRed reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsExpressionMutationWTPromoterT3GAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
Plasmid#137827PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMetYCgate
Plasmid#105074PurposeYeast mating-based Split Ubiquitin Assay, bait vector for in vivo cloningDepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GADD45 P-2/3m-luc
Plasmid#8359DepositorInsertGADD45 promoter P-2/3m (GADD45A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationP-2/3mPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2
Plasmid#191751PurposeMicrotubule marker together with nuclear envelope marker driven by bidirectional promoterDepositorUseTagsGdp1 promoter, ToxA promoter, iRFP670, and mTagBF…ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPAsp-hp
Plasmid#48122PurposeShuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoterDepositorInsertpredicted optimal signal peptide for Bacillus megaterium protein secretion
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIND-CLucZ
Plasmid#53224PurposeInducible expression of Cypridina luciferase and shRNAmir of interestDepositorInsertsCypridina luciferase
non-silencing shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)
Plasmid#163074PurposeEntry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)DepositorInsertGrx1-roGFP1-iEER
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h2
Plasmid#86691PurposeFor transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'DepositorInserthistone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)
UseTagsHAExpressionMutationPromoterT3Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Stx3-pmCitrine
Plasmid#92421PurposeExpression of syntaxin-3 C-terminally conjugated to mCitrine.DepositorInsertStx3 (Stx3 Rat)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1
Plasmid#164800PurposeFor CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1DepositorInsertsERH CRISPRi gRNA1
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2
Plasmid#164801PurposeFor CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2DepositorInsertsERH CRISPRi gRNA2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1.mir451_KO
Plasmid#164689PurposeFor CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNADepositorInsertmiR-451 CRISPR KO gRNA (MIR451A )
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern/pRSETB
Plasmid#67659Purposeorange luminescent protein for high-speed single-cell and whole body imagingDepositorInsertOrange Nano-lantern
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
sfTq2-PBP5
Plasmid#117959PurposePeriplasmic expression of superfolder mTurquoise2 (pNM067 pTHV037-DsbAss-LEGPAGL-sfTq2-EFGS-∆1-17-PBP5)DepositorInsertsfTq2-PBP5 (dacA )
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRFP1*
Plasmid#104000PurposePlasmid encoding mRFP1*DepositorInsertmRFP1*
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLAVIKEF...T…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSPYngK+-hp
Plasmid#48121PurposeShuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertcodon optimized signal peptide of YngK
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cyan Nano-lantern/pRSETB
Plasmid#67658Purposecyan luminescent protein for high-speed single-cell and whole body imagingDepositorInsertCyan Nano-lantern
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPS0384
Plasmid#133234PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cyan Nano-lantern(Ca2+)/pcDNA3
Plasmid#71344PurposeExpress Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cellDepositorInsertCyan Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo-tdTomato
Plasmid#153424PurposeRetroviral (MSCV) expression of tdTomato fluorescent proteinDepositorInserttdTomato
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRFP1
Plasmid#104001PurposePlasmid encoding mRFP1DepositorInsertmRFP1
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2A, K5E, N6D, T21S,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3LCA_TM287/288
Plasmid#132701Purposeexpresses TM287/288 in E.coli, to use as positive control in ELISADepositorUseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
RT3REVIN
Plasmid#111167PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsDsRed and mVenusExpressionMutationWTPromoterT3GAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIVT/Smarcc1
Plasmid#122145PurposeFor making Smarcc1 RNADepositorInsertSmarcc1 (Smarcc1 Mouse)
UseFor in vitro transcriptionTagsExpressionMutationPromoterT7Available sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir421-CMV2-mir1
Plasmid#164673PurposeExpress miR-421 and miR-1-1 in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL502-crRNA
Plasmid#174383PurposeThe plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterraneiDepositorInserta crRNA targeting the template strand
UseCRISPR; Archaeal expressionTagsExpressionBacterialMutationPromoterPhaR promoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His Keap
Plasmid#62457Purposeexpresses his tagged human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
UseTagsHisTagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDY-HA1-HA2-neo
Plasmid#109123PurposeFR cassette provider to introduce FR cassette to a BAC by recombineeringDepositorInsertsHA1
HA2
UseTagsExpressionMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/2K-NS4B
Plasmid#120827PurposeEctopic expression of ZIKV proteinDepositorInsert2K-NS4B ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPS0385
Plasmid#133235PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only