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Showing: 3301 - 3350 of 6304 results

  4. AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE

    Plasmid
    #196414
    Purpose
    Astrocytic expression of membrane-targeted FLAG spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smFLAG
    Use
    AAV; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and GfaABC1D
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196414-plasmid-map-sequence-id-385111

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  7. pAAV-nEF-Con/Foff DREADD Gi-mCherry

    Plasmid
    #177673
    Purpose
    Expresses DREADD hM4D (Gi) only in Cre expressing cells and not in Flp expressing cells
    Depositor
    Karl Deisseroth
    Has Service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    nEF
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177673-plasmid-map-sequence-id-348532

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  8. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51084
    Purpose
    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51084-plasmid-map-sequence-id-172966

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  9. pAAV-DIO-CAG-hM3Dq-P2A-dTomato

    Plasmid
    #202036
    Purpose
    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM3Dq-2A-dTomato
    Depositor
    Peter Jonas, Yoav Ben-Simon
    Insert
    hM3Dq
    Use
    AAV
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    202036-plasmid-map-sequence-id-405528

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  12. pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)

    Plasmid
    #105922
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITR
    Depositor
    Marcella Patrick
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Tags
    Expression
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    105922-plasmid-map-sequence-id-200599

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  19. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

    Plasmid
    #132389
    Purpose
    Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauWT 2N4R-EGFP (MAPT Human, Synthetic)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132389-plasmid-map-sequence-id-270333

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  22. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid
    #132393
    Purpose
    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Proline 301 to Leucine (P301L)
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132393-plasmid-map-sequence-id-341619

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  25. pAAV-double floxed-eNpHR-EYFP-WPRE-pA

    Plasmid
    #20949
    Purpose
    Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    halorhodopsin
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    20949-plasmid-map-sequence-id-200280

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  29. pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3

    Plasmid
    #172921
    Purpose
    For enriched expression of jGCaMP8s in axons with cytosolic expression of mRuby3
    Depositor
    GENIE Project
    Has Service
    AAV1 and AAV9
    Insert
    axon-jGCaMP8s-P2A-mRuby3
    Use
    AAV
    Tags
    GAP43 palmitoylation domain and P2A-mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin1
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    172921-plasmid-map-sequence-id-341474

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  32. pAAV-DIO-CAG-hM4Di-P2A-dTomato

    Plasmid
    #202037
    Purpose
    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato
    Depositor
    Peter Jonas, Yoav Ben-Simon
    Insert
    hM4Di
    Use
    AAV
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    202037-plasmid-map-sequence-id-405580

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  34. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Article
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U1a and U6
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112139-plasmid-map-sequence-id-217823

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  35. pAAV-hSyn-con/fon DREADD Gi-mCherry

    Plasmid
    #200660
    Purpose
    Construct for chemogenetically silencing and visualizing subpopulations of neurons in the retina
    Depositor
    Samer Hattar
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    200660-plasmid-map-sequence-id-393500

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  44. pAAV-nEF Con/Foff DREADD Gq-mCherry

    Plasmid
    #183533
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre but not Flp
    Depositor
    Karl Deisseroth
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    None
    Promoter
    nEF
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    183533-plasmid-map-sequence-id-362041

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  47. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE

    Plasmid
    #104491
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    GENIE Project, Douglas Kim
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP7s
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104491-plasmid-map-sequence-id-200288

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Showing: 3301 - 3350 of 6304 results