-
Plasmid#182308PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mAppleInsertsUseTagsT2A-mycNLS-mAppleExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only
-
pDGE6
Plasmid#79476PurposesgRNA shuttle vector; module 1EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC017 - LwCas13a guide expression backbone with tRNAval promoter
Plasmid#91907PurposeBackbone for cloning LwCas13a guides under tRNAval promoterDepositorInsertLwCas13a Guide site
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS006
Plasmid#211506PurposeAAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequencesDepositorTypeEmpty backboneUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
PAX6 donor plasmid
Plasmid#105239PurposeKnock in H2B-GFP into the human PAX6 locusDepositorInsertPAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
UseTALENTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBL728
Plasmid#140398PurposeExpresses CsoR-regulated 3WJdB RNA aptamer driven by T7 promoter with the csoO sequence 5BP downstream from the promoterDepositorInsertT7-csoO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL716
Plasmid#140386PurposeExpresses MphR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mphO sequence 5BP downstream from the promoterDepositorInsertT7-mphO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2
Plasmid#175499PurposeAll-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID5XY
Plasmid#165051PurposeIntegrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…PromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD317 (Pmyo-2::GFP + SEC)
Plasmid#73344PurposeSEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology armsDepositorInsertPmyo-2::GFP + SEC
UseCRISPR and Cre/LoxTagsGFPExpressionWormMutationPromotermyo-2Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-rtTA-IRES-eGFP-pGK-NeoR
Plasmid#204497PurposeExpresses rtTA and Neomycin resistance genes in mammalian cellsDepositorInsertrtTA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNC1124
Plasmid#41560DepositorInsertUra3-GAL1-UBIYdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
AA010
Plasmid#216038PurposeFragmid fragment: (C' terminus) for C>T base editingDepositorHas ServiceCloning Grade DNAInsertUGI_v1.1; NpNLS_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMGS58
Plasmid#135311PurposeA HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminalDepositorInsertHygR-P2A-AID
UseN terminal tagging plasmidTagsExpressionMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
UseTagsExpressionBacterialMutationPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Aurli_150841_GZG
Plasmid#162563PurposeInactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.DepositorInsertshble
UseUnspecifiedTagsExpressionMutationPromoterGAPDHAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDD283
Plasmid#66824PurposeYPET^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertYPET-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized YPETExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pADH99
Plasmid#90979PurposeNAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.DepositorInsertsUS_HIS1
FRT
pENO1
C. albicans Cas9
pMAL2
FLP
NAT 1 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
AX53_pS.Donor.S1.TS
Plasmid#100290PurposeAAVS1-targeting plasmidDepositorInsert18 bp RFLP (MluI+KspAI)
UseCRISPR and Synthetic Biology; Human targetingTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pP2A_NLS_sfGFP
Plasmid#63709PurposeExpressing P2A_NLS_sfGFP with homologous arm of Nanog.DepositorInsertNanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
UseJust a donor dna with nanog homologous arm for kn…TagssfGFPExpressionMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
UseTagsExpressionBacterialMutationPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGE656
Plasmid#153254PurposeM2 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS29: pDonor(PmcCAST)
Plasmid#168162PurposePmcCAST donor harboring non-functional cargo.DepositorInsertsPmcCAST right end (RE)
PmcCAST left end (LE)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PU6::unc-119_sgRNA
Plasmid#46169Purposeunc-119 targeting sgRNADepositorInsertunc-119 targeting sgRNA (unc-119 Synthetic)
UseCRISPRTagsExpressionMutationPromoterC. elegans U6 snRNA pol III promoterAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
CAP-AAV.FUS.3
Plasmid#222333PurposeAAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.DepositorInsertAAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1
UseAAVTagsExpressionMutationPromoterp41Available sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTX1-SMN1-exon7-ESE1
Plasmid#126041PurposeIn-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysisDepositorInsertSMN1 exon7 ESE1
UseTagsExpressionMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGE474
Plasmid#153265PurposeM1 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE475
Plasmid#153266PurposeM2 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsaI
Plasmid#128649PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5135: pCAG-BFP-GuideArray
Plasmid#210606PurposeGuide array for hyperdCas12a-based transcriptional activator with AAAT synSeparatorDepositorInsertpCAG-BFP-GuideArray
UseCRISPRTagsExpressionMutationPromoterCAGAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
hOC-GFPtpz
Plasmid#110207PurposeExpresses GFP in mature bone cells.DepositorInserteGFPtopaz
UseTagsNoneExpressionMammalianMutationPromoterHuman osteocalcin (BGLAP)Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pADH100
Plasmid#90980PurposeNAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENO1-iRFP-NATr
Plasmid#129731PurposeIntegration plasmid to overexpress iRFP in Candida albicansDepositorInsertiRFP-Candida albicans codon-optimized
UseTagsExpressionYeastMutationPromoterENO1 from Candida albicansAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_RFP
Plasmid#136979PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker RFP
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
HBT-GCaMP6-HA
Plasmid#107433PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertGCaMP6S
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
tol2-CAG::ChR2-YFP
Plasmid#59740PurposeOptogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)DepositorInsertChannelrhodopsin-2
UseTagsYFPExpressionMammalianMutationH134RPromoterCAGAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-hEF1A-MG-U6-sgRNA
Plasmid#175505PurposeAll-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhEF1a, U6Available sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PU6::klp-12_sgRNA
Plasmid#46170Purposeklp-12 targeting sgRNADepositorInsertklp-12 targeting sgRNA (klp-12 Synthetic)
UseCRISPRTagsExpressionWormMutationPromoterC. elegans U6 snRNA pol III promoterAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
S9_attL1-EGFP-C-term-attL2
Plasmid#128473PurposeEGFP entry cloneDepositorInsertEGFP entry clone
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
AA009
Plasmid#216037PurposeFragmid fragment: (C' terminus) Nucleoplasmin NLSDepositorHas ServiceCloning Grade DNAInsertNpNLS_v1.1; 3xHA_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pADH118
Plasmid#90983PurposeNAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. albicans LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH137
Plasmid#90986PurposeNAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.DepositorInsertsC. albicans LEU2 1 of 2
pENO1
Cas9
NAT 1 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLinker-AID-3xHA-DHFR-LoxP
Plasmid#86669Purposefor generation of AID strains using CRISPR tagging technologyDepositorInsertLinker-AID-3xHA-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFH75
Plasmid#128224Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
AY42_pS.Donor.R5
Plasmid#100291PurposeCCR5-targeting plasmidDepositorInsert18 bp RFLP (MluI+KspAI)
UseCRISPR and Synthetic Biology; Human targetingTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLinker-AID-Ty-HXGPRT-LoxP
Plasmid#86667Purposefor generation of AID strains using CRISPR tagging technologyDepositorInsertLinker-AID-Ty-HGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH143
Plasmid#90989PurposeNAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH140
Plasmid#90988PurposeNAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.DepositorInsertsC. maltosa LEU2 1 of 2
pENO1
Cas9
NAT 1 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH119
Plasmid#90985PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.DepositorInsertgRNA conserved, C. albicans LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
P2A-NLS-tdtomato-EPG backbone
Plasmid#215548PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only