We narrowed to 3,053 results for: PPL;

1. pJMP8930

   Plasmid

   #220905

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA.PKD.K/W

   Plasmid

   #10809

   Depositor

   Alex Toker

   Article

   Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

   Insert

   PKD (PRKD1 Human)
   

   Use

   Tags

   HA and Myc (inserted between amino acids 33&3…

   Expression

   Mammalian

   Mutation

   Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

   Promoter

   CMV

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

3. HA.PKD.S738E/S742E

   Plasmid

   #10810

   Depositor

   Alex Toker

   Article

   Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

   Insert

   PKD (PRKD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Activation loop active: S738E and S742E (bp 2212-…

   Promoter

   CMV

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pSB700 lenti gRNA Blasto_zhang2.0

   Plasmid

   #167912

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2,0_3E5E_eGFP

   Plasmid

   #69359

   Purpose

   Encodes for Ebola Minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

   Insert

   3E5E eGFP minigenome
   

   Use

   T7

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_Puro

   Plasmid

   #167863

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKB01_tagRFP

   Plasmid

   #69103

   Purpose

   Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   tagRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmRuby2-CrowdingLinker-Clover

   Plasmid

   #171058

   Purpose

   Expresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.

   Depositor

   Ke Xu

   Article

   Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi

   Insert

   Crowdedness-sensitive linker domain
   

   Use

   Tags

   mClover2 and mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-memGFP-P2A

   Plasmid

   #80809

   Purpose

   Fyn myristoylation domain fused to N-terminus of GFP (memGFP) without stop codon, followed by P2A

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   pME-memGFP-P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTi4.0_SpdCas9_pmcDA1

    Plasmid

    #204613

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUCBB-ctH6-eGFP

    Plasmid

    #32562

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    6 His tag with Thrombin site

    Expression

    Mutation

    Promoter

    Consitutive lac

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB700 lenti gRNA Puro_zhang2.0

    Plasmid

    #167919

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRC3EGFP

    Plasmid

    #193790

    Purpose

    Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PR promoter

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbdCas9S_P0526-sgRNAmreB

    Plasmid

    #149654

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, P0526

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB_TRE_Z17-PDGFR_EF1a_mCherry

    Plasmid

    #186303

    Purpose

    Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    Z17-PDGFR
    mCherry
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-FLEX-VAMP2:HRP

    Plasmid

    #60659

    Purpose

    Labels synaptic vesicles for electron microscopy

    Depositor

    Scott Sternson

    Article

    Atasoy et al Nat Neurosci. 2014 Dec;17(12):1830-9. do

    Insert

    VAMP2:HRP
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pUCBB-pT7-eGFP

    Plasmid

    #32552

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    pT7

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. NAX_CAGGS_CAS9_m4_Blasto

    Plasmid

    #167864

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPpT4- PGK1prom-TIR1-FLAG

    Plasmid

    #189724

    Purpose

    Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1 promoter

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC-crRNA-Km

    Plasmid

    #158712

    Purpose

    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    AcGFP (for crRNA cloning)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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