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pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP

Plasmid

#192609

Purpose

Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS-P2A-tagBFP

Use

Lentiviral and Synthetic Biology

Tags

tagBFP

Expression

Mutation

Promoter

PGK

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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sg2.02 MUC4.1

Plasmid

#101152

Purpose

gRNA with two MCP binding sites

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

mU6

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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mCh-Miro1(∆TM)-SspB

Plasmid

#194007

Purpose

Optogenetic tool

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Miro1 (var4, peroxisomal splice variant) (RHOT1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYn-DIO-ScramblemiR-IRES-mCitrine

Plasmid

#186418

Purpose

Expression of scrambled shRNA used as a control for knock down experiments

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

ScramblemiR

Use

AAV

Tags

mCitrine

Expression

Mutation

Promoter

human Synapsin1

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine

Plasmid

#186419

Purpose

Expression of shRNA for the knock down of mouse Egr3

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

miREgr3-5D

Use

AAV

Tags

mCitrine

Expression

Mutation

Promoter

human Synapsin1

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAL928

Plasmid

#119853

Purpose

precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C

Use

Tags

none

Expression

Bacterial

Mutation

Cysteine 169 to Serine, Cysteine 179 to Serine, C…

Promoter

T7

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-mMLKL

Plasmid

#208784

Purpose

Inducibly expresses mouse MLKL fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mMLKL Cry2 (Mlkl Mouse)

Use

Lentiviral

Tags

Cry2 and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-mRipK3

Plasmid

#208783

Purpose

Inducibly expresses mouse RipK3 fused to light activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mRipK3 Cry2 (Ripk3 Mouse)

Use

Lentiviral

Tags

Cry2 and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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EC71171

Plasmid

#154069

Purpose

Level 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backbone

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

CRE_U5intron

Use

Synthetic Biology

Tags

Expression

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-RedO-Txnip(C247S)

Plasmid

#206341

Purpose

AAV plasmid expressing mutant TXNIP in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

Insert

Txnip (Txnip Mouse)

Use

AAV

Tags

Expression

Mutation

C247S

Promoter

human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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cpLOV2-V7

Plasmid

#168993

Purpose

circularly permuted LOV2 variant-V7

Depositor

Yubin Zhou

Article

He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

Insert

cpLOV2-V7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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cpLOV2-V5

Plasmid

#168991

Purpose

circularly permuted LOV2 variant-V5

Depositor

Yubin Zhou

Article

He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

Insert

cpLOV2-V5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-I-PhoCl-HCVp

Plasmid

#87697

Purpose

Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)

Use

Tags

EBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLncEXP

Plasmid

#64865

Purpose

Empty vector for lncRNA expression

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
dCas9-CRY2Clust

Plasmid

#200969

Purpose

induction of phase-separated condensate by CRY2Clust

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

CRY2Clust

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pOME0207

Plasmid

#178407

Purpose

KSHV pre-miRNA K12-2wt

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K 12

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0208

Plasmid

#178408

Purpose

KSHV pre mir K12-4wt

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K 12

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0209

Plasmid

#178409

Purpose

KSHV pre microRNA K12-5wt

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K 12

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME210MR

Plasmid

#178410

Purpose

KSHV pre microRNA K12 6wt

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K 12

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0211

Plasmid

#178411

Purpose

KSHV micro RNA K12-7wt

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K 12

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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