We narrowed to 28,416 results for: REP

1. pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA

   Plasmid

   #139513

   Purpose

   B6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   CAG rox stop rox td-sfGFP dre reporter
   

   Use

   Mouse Targeting; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

   Plasmid

   #162967

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Dual Strep2 purification tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115220

   Purpose

   Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   Pin3Cas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

   Plasmid

   #62577

   Purpose

   Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115210

   Purpose

   Expresses AspCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   AspCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115214

   Purpose

   Expresses PauCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PauCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET StrepII TEV co-transformation cloning vector (13K-R)

   Plasmid

   #48319

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   StrepII

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

   Plasmid

   #227611

   Purpose

   Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   MBP, 10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-88 only

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

   Plasmid

   #227610

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-400, 706-757 [TM and HB2 deleted]

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid

    #208626

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 191~307, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid

    #208627

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 299~398, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid

    #208629

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 212~285, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-luciferase-reporter-CHAF1B-SVA-del-VNTR

    Plasmid

    #202830

    Purpose

    pGL3 luciferase reporter with CHAF1B-SVA-del-VNTR sequence cloned upstream of SV40 promoter to test effects of SVA-del-VNTR insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    CHAF1B intronic SVA del-VNTR sequence
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-EF1alpha-nCoV2019-N-YBBR-2xStrep-IRES-Puro

    Plasmid

    #185450

    Purpose

    Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal YBBR and Strep tag in mammalian cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

    Insert

    Nucleocapsid (N SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    Strep-Tag II and YBBR

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pB6-Hipp11-tdTomato cre reporter-FNF-DTA

    Plasmid

    #183024

    Purpose

    B6J Hipp11-pCAG-loxP-FNF-bGHpA-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-2xFLAG-2xSTREP-CCND1(T286I)-IRES-mCherry

    Plasmid

    #172638

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286I) and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ile

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-2xFLAG-2xSTREP-CCND1(P287L)-IRES-mCherry

    Plasmid

    #172636

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287L) and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Pro287Leu

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

    Plasmid

    #162937

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6 fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C514-E42: attB5r-RU5u-Strep2-nostop-attB1r

    Plasmid

    #162961

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Strep2 purification tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

    Plasmid

    #62580

    Purpose

    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

    Promoter

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only