We narrowed to 13,482 results for: cas9 genes

1. pX330-Flag-dHeFSpCas9

   Plasmid

   #92116

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   Cbh

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyCas9 PE2-3HA

   Plasmid

   #169853

   Purpose

   Mammalian Expression, SpyCas9 prime editor with 3HA-tag

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpCas9-H840A-M-MLV-3HA
   

   Use

   Tags

   3XHA tag and bpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. V1-MESA-45F-M-dCas9

   Plasmid

   #84504

   Purpose

   MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

   Depositor

   Joshua Leonard

   Article

   Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

   Insert

   V2-MESA-35F-M-dCas9
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. A3F-Cas9n-UGI

   Plasmid

   #119138

   Purpose

   Base editor made from APOBEC3F

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3F-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459V2.0-SpCas9-HF4

   Plasmid

   #108295

   Purpose

   pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV dCas9-MC (eGFP)

   Plasmid

   #89931

   Purpose

   Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   dCas9-MC
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deactivated Cas9, fragment of M.SssI (residues 27…

   Promoter

   CMV

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpRY dCas9 VPR

   Plasmid

   #188511

   Purpose

   Expresses FLAG tagged SpRY dCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. A3C-Cas9n-UGI

   Plasmid

   #119136

   Purpose

   Base editor made from APOBEC3C

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3C-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXLE-dCas9GFP-shP53

   Plasmid

   #102906

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBLO 9.2_bCas9-CP199

    Plasmid

    #121496

    Purpose

    TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p15A

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330 NC-FKBP-Cas9

    Plasmid

    #162725

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

    Plasmid

    #107730

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

    Plasmid

    #107729

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. BII-gR-PtW-eSpCas9

    Plasmid

    #133357

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Use

    Tags

    HA-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. A3G-Cas9n-UGI

    Plasmid

    #119139

    Purpose

    Base editor made from APOBEC3G

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3G-Cas9 nickase-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330_SpCas9_USP7 Exon 3 gRNA

    Plasmid

    #131257

    Purpose

    pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    humanised S. pyogenes Cas9 nuclease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2+-dCas9-olEzh2

    Plasmid

    #180216

    Purpose

    Ezh2 fused to dCas9 for H3K27me3 epigenome editing

    Depositor

    Hiroyuki Takeda

    Article

    Fukushima et al Epigenetics Chromatin. 2019 Mar 13;12(1)

    Insert

    enhancer of zeste 2 polycomb repressive complex 2 subunit
    

    Use

    Tags

    FLAG and dCas9

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAT15542_nCBE-SuperFi-Cas9

    Plasmid

    #184372

    Purpose

    Mammalian expression of SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAT15544_nABE-SuperFi-Cas9

    Plasmid

    #184374

    Purpose

    Mammalian expression of SuperFi-Cas9 ABE7 base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nABE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    FLAG, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS315e_pGal-dCas9-SH3

    Plasmid

    #79613

    Purpose

    Expresses dCas9-SH3 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    SH3 domain

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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