2,223 results

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p5E LyzC

Plasmid

#135200

Purpose

Neutrophil specific label

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

Lyz promoter

Use

Synthetic Biology

Promoter

LyzC

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-B-actin

Plasmid

#117814

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:KIT K642E

Plasmid

#118849

Purpose

Expresses human KIT K642E mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

KIT K642E (KIT Zebrafish, Human)

Use

Zebrafish expression, tol5

Mutation

KIT K642E

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDrVSP(C302S)-IRES2-EGFP

Plasmid

#80331

Purpose

Expresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cell

Depositor

Yasushi Okamura

Article

Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

Insert

Daino rerio voltage-sensing phosphatase (tpte Zebrafish)

Expression

Mammalian

Mutation

C302S

Promoter

CMV

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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14UAS zfPSD95:GFP

Plasmid

#74314

Purpose

A fusion of zebrafish PSD95 to GFP under control of 14 UAS repeats

Depositor

Martin Meyer, Stephen Smith

Article

Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

Insert

zebrafish PSD95

Tags

EGFP

Mutation

Y599H and I793S (please see depositor comment bel…

Promoter

UAS

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
Zebrafish-gRNA-0005

Plasmid

#42245

Purpose

gRNA targeted to zebrafish gene gsk3b

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-gsk3b (gsk3b Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-Pcdh19-GFP

Plasmid

#35675

Depositor

James Jontes

Article

Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

Insert

Pcdh19 (pcdh19 Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
zWnt5b

Plasmid

#21282

Depositor

Randall Moon

Article

Stoick-Cooper et al Development. 2007 Feb . 134(3):479-89.

Insert

Wnt5b (wnt5b Zebrafish)

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only

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ZP17 Krox20

Plasmid

#16924

Depositor

Randall Moon

Insert

Krox20 (egr2b Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
ZE45 Dvl-2 pCS2P+

Plasmid

#17086

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Dvl-2 (dvl2 Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
Tol2-kdrl:mCherry-caax

Plasmid

#194287

Purpose

Expression plasmid for zebrafish or Danionella cerebrum

Depositor

Pui-Ying Lam

Article

Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049

Insert

kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)

Use

Zebrafish or danionella cerebrum expression

Tags

mCherryCAAX from the Tol2kit (Kwan et al., 2007)

Promoter

kdrl(flk1)

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2--3.5ubb:plin2-tdtomato

Plasmid

#172534

Purpose

Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

-3.5ubb:plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F and R415H mutations obse…

Promoter

zebrafish ubb

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

Plasmid

#140892

Purpose

Expresses modified Danio rerio VSP with enhanced phosphatase activity fused with mCherry in mammalian cells.

Depositor

Yasushi Okamura

Article

Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

Insert

CiDr-VSP L223F-mCherry (tpte, vsp Zebrafish, Ciona intestinalis)

Tags

mCherry

Expression

Mammalian

Mutation

changed 223L to F in Dr-VSP (zebrafish TPTE)

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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p5E mpeg1

Plasmid

#135199

Purpose

Macrophage specific label

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

mpeg1 promoter

Use

Synthetic Biology

Promoter

mpeg1

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-Gal4-Vp16-Crys-CFP

Plasmid

#97144

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Gal4-Vp16

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c

Plasmid

#107599

Purpose

An entry vector with U6a and U6c promoter driving guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Inserts

U6a promoter
U6c promoter

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-mito-Dendra2

Plasmid

#107590

Purpose

Expresses mitochondria localized Dendra2 in zebrafish neutrophils

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

mito-Dendra2

Tags

mitochondira localization sequence

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMT-sox10-cytoBirA-2A-mCherry_Ras

Plasmid

#80062

Purpose

Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Sox10 promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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acta2_GFP_pA_pDestTolpA2

Plasmid

#78684

Purpose

acta2:GFP transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Promoter

acta2

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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ZP27 Bmp2b

Plasmid

#16915

Depositor

Randall Moon

Insert

BMP2b (bmp2b Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only