2,162 results

1. Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

   Plasmid

   #218205

   Purpose

   Zebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

   Depositor

   Katarzyna Koltowska

   Article

   Panara et al Development. 2024 May 1;151(9):dev202525

   Insert

   -2.1prox1a
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST mpeg1:tomato-CAAX

   Plasmid

   #135215

   Purpose

   Red tagged macrophages

   Depositor

   David Tobin

   Article

   Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

   Insert

   mpeg1 and tdTomato
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK036 desmaMCS:minCerulean,cryaa:Venus

   Plasmid

   #195981

   Purpose

   Expression construct

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, cryaa Venus (desma Zebrafish)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5E-dr-myl7-pro (JDW 1148)

   Plasmid

   #229836

   Purpose

   A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   cmlc2/myl7
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-NLS-Cre-2A-mCherry-FKF

   Plasmid

   #119868

   Purpose

   Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2-cryR;cldn5bP1Egfp

   Plasmid

   #90158

   Purpose

   contains cldn5b specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   cldn5bP1Egfp (cldn5b Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. zCREST3:redCas9; control_gRNA

   Plasmid

   #199334

   Purpose

   pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   zCREST3
   Cas9
   control sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKHR8

   Plasmid

   #74625

   Purpose

   a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.

   Depositor

   David Grunwald

   Article

   Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

   Inserts

   FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
   cmlc2::mCherry
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   alpha-crystallin and cmlc2

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS Zpapc

   Plasmid

   #25774

   Depositor

   Edward De Robertis

   Article

   Yamamoto et al Development. 1998 Sep . 125(17):3389-97.

   Insert

   Papc (pcdh8 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pT2KXIG (Tol2)-tp63:ikk1KD:GFP

    Plasmid

    #105645

    Purpose

    Kinase-deleted Ikk1(alpha) expression in basal keratinocytes

    Depositor

    Sandra Rieger

    Article

    Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

    Insert

    tp63:ikk1KD:GFP (chuk Zebrafish)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    p63 promoter + ikk1 (kinase domain deleted) + GFP

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tol2-lyzC-mito-Dendra2

    Plasmid

    #107590

    Purpose

    Expresses mitochondria localized Dendra2 in zebrafish neutrophils

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    mito-Dendra2
    

    Use

    Tags

    mitochondira localization sequence

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT2KXIG (Tol2)-tp63:ikk1:GFP

    Plasmid

    #105646

    Purpose

    Ikk1(alpha) expression in basal keratinocytes

    Depositor

    Sandra Rieger

    Article

    Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

    Insert

    tp63:ikk1:GFP (chuk Zebrafish)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p5E-gfap

    Plasmid

    #82401

    Purpose

    Gateway-compatible zebrafish gfap promoter

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    gfap promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT-TODestRFP_RNAseH1

    Plasmid

    #65785

    Purpose

    expresses RFP-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Human, Zebrafish)
    

    Use

    Tags

    RFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry

    Plasmid

    #230043

    Purpose

    Partial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

    Depositor

    Sumeet Pal Singh

    Article

    Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

    Insert

    lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    fabp10a

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E_he1a:mCherry

    Plasmid

    #113881

    Purpose

    p3E entry vector for Three Multisite Gateway

    Depositor

    Marnie Halpern

    Insert

    he1a (he1.1 Zebrafish)
    

    Use

    Zebrafish danio expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 5UAS zfSynaptophysin:GFP

    Plasmid

    #74316

    Purpose

    A fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeats

    Depositor

    Stephen Smith, Martin Meyer

    Article

    Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

    Insert

    synaptophysin
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Q12H and deletion of F13 (please see depositor co…

    Promoter

    5UAS

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT-TODestFLAGHA_RNAseH1

    Plasmid

    #65782

    Purpose

    expresses FLAGHA-tagged RNAsH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Human, Zebrafish)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3E-U6a-polg guide

    Plasmid

    #107598

    Purpose

    An entry vector with U6a promoter driving polg guide RNA expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    polg guide RNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ZE31 Prickle-1-pCS2P+

    Plasmid

    #17072

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    Prickle-1 (prickle1a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only