2,162 results

1. pTol2p2A--3mnx1:mTagBFP-NS-MCS

   Plasmid

   #78250

   Purpose

   Can be used to subclone in gene of interest into blue motor neuron expression vector.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Inserts

   -3mnx1 (mnx1 Zebrafish)
   -mTagBFP-NS-MCS
   

   Use

   Zebrafish motor neuron expression

   Tags

   Expression

   Mutation

   Promoter

   -3mnx1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-sox10-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80062

   Purpose

   Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Sox10 promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDrVSP(C302S)-IRES2-EGFP

   Plasmid

   #80331

   Purpose

   Expresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cell

   Depositor

   Yasushi Okamura

   Article

   Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

   Insert

   Daino rerio voltage-sensing phosphatase (tpte Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C302S

   Promoter

   CMV

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+cPLA2-mKate2

   Plasmid

   #162068

   Purpose

   Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Avian, xenopus, zebrafish

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. tol2-mpx-lyn-GFP

   Plasmid

   #35037

   Depositor

   Anna Huttenlocher

   Article

   Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

   Insert

   Lyn-GFP (lyn Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCS2+ centrin-mKate2

   Plasmid

   #105941

   Purpose

   in vitro transcription, zf centrin N-terminally tagged with mkate2

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   Centrin
   

   Use

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Zebrafish aBb crystallin 2kb promoter/AcGFP

   Plasmid

   #98099

   Purpose

   Zebrafish aBb crystallin 2kb promoter segment driving GFP expression

   Depositor

   Mason Posner

   Article

   Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

   Insert

   Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)
   

   Use

   Gfp expressing

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. HS-Par3-GFP

   Plasmid

   #105947

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   Par3
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5'Entry-lck-7.4kb

   Plasmid

   #58891

   Purpose

   provides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenes

   Depositor

   Jeffrey Yoder

   Insert

   zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
   

   Use

   Gateway 5' entry clone

   Tags

   Expression

   Mutation

   mutated single nucleotide (T-->C, 1874th nucle…

   Promoter

   lymphocyte-specific protein tyrosine kinase (lck)…

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. hsp70l: hAPOB48-mCherry.

    Plasmid

    #140639

    Purpose

    Heat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.

    Depositor

    Steven Farber

    Article

    Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119

    Inserts

    hsp70l (hsp70l Zebrafish)
    APOB (APOB Human)
    

    Use

    Zebrafish expression

    Tags

    mCherry

    Expression

    Mutation

    APOB48: amino acid 1-2180

    Promoter

    hsp70l

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKHR8

    Plasmid

    #74625

    Purpose

    a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Inserts

    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    cmlc2::mCherry
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    alpha-crystallin and cmlc2

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-Opto-Acvr1ba-P2A-Acvr2ba

    Plasmid

    #232640

    Purpose

    Optogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expression

    Depositor

    Katherine Rogers

    Insert

    Acvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E_he1a:eYFP

    Plasmid

    #113879

    Purpose

    p3E entry vector for Three Multisite Gateway

    Depositor

    Marnie Halpern

    Insert

    he1a (he1.1 Zebrafish)
    

    Use

    Zebrafish danio expression

    Tags

    eYFP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5E-gfap

    Plasmid

    #82401

    Purpose

    Gateway-compatible zebrafish gfap promoter

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    gfap promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. p5E-3x125bp-mnx1

    Plasmid

    #169043

    Purpose

    5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    Mnx1 enhancer (3x) (mnx1 Zebrafish)
    

    Use

    Gateway entry cloning vector

    Tags

    Expression

    Mutation

    Promoter

    3xMnx1

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST mfap4:turquoise2

    Plasmid

    #135218

    Purpose

    Cyan tagged macrophages

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter and mTurquoise2
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST mpeg1:tomato-CAAX

    Plasmid

    #135215

    Purpose

    Red tagged macrophages

    Depositor

    David Tobin

    Article

    Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

    Insert

    mpeg1 and tdTomato
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2

    Plasmid

    #119061

    Purpose

    Fluorescent vector with zebrafish pax3a enhancer 5; Tol2 version

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    pax3a_enh5-E1b:eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E1b minimal promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-Trpa1b-2A-mCherry

    Plasmid

    #106291

    Purpose

    Gateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherry

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Insert

    Trpa1b (trpa1b Zebrafish)
    

    Use

    Gateway middle entry

    Tags

    2A-mCherry

    Expression

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Phat2-dr-cPla2-C2-EGFP-6H

    Plasmid

    #187001

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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