2,159 results

1. pKHR8

   Plasmid

   #74625

   Purpose

   a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.

   Depositor

   David Grunwald

   Article

   Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

   Inserts

   FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
   cmlc2::mCherry
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   alpha-crystallin and cmlc2

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2-lyzC-mcherry-2a-CDK2

   Plasmid

   #121131

   Purpose

   neutrophil specific expression

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-WT CDK2 (cdk2 Zebrafish)
   

   Use

   Tags

   mcherry-2a

   Expression

   Bacterial

   Mutation

   Promoter

   neutrophil specific lyzC

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUFlip-floxed2A-KalTA4-; HE1A:BFP -0

   Plasmid

   #180010

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; HE1A: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

   Plasmid

   #180011

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; HE1A: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

   Plasmid

   #180012

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; HE1A: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3e UbB polyA

   Plasmid

   #188702

   Purpose

   Gateway 3' element encoding the ubiquitin B polyadenylation sequence

   Depositor

   David Tobin

   Article

   Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

   Insert

   UbB polyA (ubb Zebrafish)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-gfap

   Plasmid

   #82401

   Purpose

   Gateway-compatible zebrafish gfap promoter

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   gfap promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3E_he1a:mCherry

   Plasmid

   #113881

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPRISM-gal4-VP16-gcry-TagBFP

   Plasmid

   #117777

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   gal4/VP16
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. hsp70: mKate2-DN Kif5a

    Plasmid

    #105972

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    Kif5a (kif5aa Zebrafish)
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    truncation of aa416-1033

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5E mpeg1

    Plasmid

    #135199

    Purpose

    Macrophage specific label

    Depositor

    David Tobin

    Article

    Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

    Insert

    mpeg1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    mpeg1

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1

    Plasmid

    #162566

    Purpose

    Used for the dual color labeling of cPLA2 and LaminB1 expression.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Inserts

    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    Lamin B1 (lmnb1 Zebrafish)
    

    Use

    Tags

    EGFP, P2A, and mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SV40

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p5E-smyhc1

    Plasmid

    #126748

    Purpose

    Zebrafish slow myosin heavy chain promoter. Clone 667

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    smyhc1 (smyhc1 Zebrafish)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. ins:CFP-NTR

    Plasmid

    #66593

    Purpose

    Specific and conditional cell ablation of beta cells in the zebrafish pancreas

    Depositor

    Didier Stainier

    Article

    Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.

    Insert

    Insulin promotor driving CFP-nitroreductase
    

    Use

    Zebrafish expression

    Tags

    CFP fused with N-terminus of nitroreductase

    Expression

    Mutation

    Promoter

    Zebrafish Insulin promoter (approximately 1.2kb)

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Tg (mpx:mCherry-2A-Rac2D57N)

    Plasmid

    #58934

    Purpose

    expresses dominant negative rac2 tagged with mcherry in neutrophils

    Depositor

    Anna Huttenlocher

    Article

    Deng et al Dev Cell. 2011 Oct 18;21(4):735-45. doi:

    Insert

    mcherry-2a-Rac2D57N (rac2 Zebrafish)
    

    Use

    Tags

    mcherry2a

    Expression

    Mutation

    changed Aspartate 57 to Asparagine

    Promoter

    mpx

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tol2-kdrl:mCherry-caax

    Plasmid

    #194287

    Purpose

    Expression plasmid for zebrafish or Danionella cerebrum

    Depositor

    Pui-Ying Lam

    Article

    Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049

    Insert

    kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)
    

    Use

    Zebrafish or danionella cerebrum expression

    Tags

    mCherryCAAX from the Tol2kit (Kwan et al., 2007)

    Expression

    Mutation

    Promoter

    kdrl(flk1)

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKE13-ISceI-fabp10a:CreERT2

    Plasmid

    #198242

    Purpose

    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinase

    Depositor

    Didier Stainier

    Article

    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

    Inserts

    Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA (fabp10a Zebrafish)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MiniCoopR mitfa:BRAF

    Plasmid

    #118846

    Purpose

    Expresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    BRAF V600E (BRAF Zebrafish, Human)
    

    Use

    Zebrafish expression, tol2

    Tags

    Expression

    Mutation

    BRAF V600E

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUFlip-floxed2A-mRFP-; gcry1:BFP -2

    Plasmid

    #173888

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Zebrafish ArcLight Q175

    Plasmid

    #53616

    Purpose

    Genetically encoded voltage sensor ArcLight with improved kinetics

    Depositor

    Vincent Pieribone

    Article

    Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

    Insert

    Zebrafish ArcLight-Q175 (tpte Zebrafish, Aequorea victoria, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dr-VSD contains R153Q mutation and an amino acid …

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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