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Plasmid#111327PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutation3' splice site AcAc mutation in intronPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(BrG)
Plasmid#111326PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site A to G mutation in intronPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(BrC)
Plasmid#111325PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site A to C mutation in intronPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 to gap
Plasmid#49158PurposeTemplate for generating gene 60 mRNA, sequence 5' of coding gap onlyDepositorInsertBacteriophage T4 gene 60
UseTagsExpressionMutationCoding gap and downstream sequence have been del…PromoterT7Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19 - T7 pro - IRES - EGFP
Plasmid#138586PurposeExpress EGFP with an upstream T7 promoter in pUC19 backboneDepositorInsertEGFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)
Plasmid#171129PurposeLwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)DepositorInsertU6 promoter entry plasmid for LwaCas13a crRNA cloning
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-mVenus-FLAG:Kan
Plasmid#211830PurposePlasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.DepositorInsertmVenus-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19.TRIM28.FKBP.knock-in.mRuby2
Plasmid#185008PurposeFor knocking in FKBP F36V P2A mRuby2DepositorInsertFKBP F36V P2A mRuby2
UseCloningTagsExpressionMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-URA3-3'SEC13-GFP
Plasmid#25445DepositorInsertSec13-Green Fluorescent Protein
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC19.TRIM28.FKBP.knock-in.mCerulean
Plasmid#185009PurposeFor knocking in FKBP F36V P2A mCeruleanDepositorInsertFKBP F36V P2A mCerulean
UseCloningTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAmCherry-FLAG:cat
Plasmid#211832PurposePlasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.DepositorInsertPAmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p1977 (pUC19-PTDH3-PTEF1)
Plasmid#78230PurposeExample plasmid containing a bidirectional promoter of pTDH3 and pTEF1DepositorInsertBidirectional promoters pTDH3-pTEF1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19, containing H2-Kd Balb/c WK10
Plasmid#102642PurposeEncodes sequence of the murine H2-Kd alleleDepositorInsertH2-Kd Balb/c
UseTagsExpressionMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-mCherry-FLAG:cat
Plasmid#211831PurposePlasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.DepositorInsertmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAGFP-FLAG:Kan
Plasmid#211834PurposePlasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.DepositorInsertPAGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TSV (linearized in VP2)
Plasmid#70032PurposepUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding regionDepositorInsertTSV full genome, linearized in VP2 gene
UseCloning vectorTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19 ACMV DNA-B 1.5mer
Plasmid#159135PurposeInfectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-BDepositorInsertACMV DNA-B 1.5mer
UseInfectious clone for bombarding plantsTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top-Toehold-mCherry
Plasmid#87318PurposeExpresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.DepositorInsertSplit-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~Bottom-T
Plasmid#87315PurposeControl plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.DepositorInsertSplit-Broccoli system (promoter control)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 delta gap
Plasmid#49091PurposeTemplate for generating gene 60 mRNA, coding gap deletionDepositorInsertBacteriophage T4 gene 60
UseTagsExpressionMutationCoding gap and coding gap STOP have been deleted.PromoterT7Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mishler2014-No-aptamer-controlluciferase-pUC19
Plasmid#63856PurposeThis pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromotertacAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19_msfGFP-EWSR1-repair-tempate
Plasmid#237682PurposeFor knock-in msfGFP to EWSR1 locusDepositorInsertEWSR1 (EWSR1 Human)
UseCRISPRTagsmsfGFPExpressionMutationPromoterAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19_msfGFP-TCOF1-repair-template
Plasmid#237632PurposeFor knock-in msfGFP to TCOF1 locusDepositorInsertTCOF1 (TCOF1 Human)
UseCRISPRTagsmsfGFPExpressionMutationPromoterAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19_msfGFP-FUS-repair-tempate
Plasmid#237684PurposeFor knock-in msfGFP to FUS locusDepositorInsertFUS (FUS Human)
UseCRISPRTagsmsfGFPExpressionMutationPromoterAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19::ZmUBI-RWT4Ca+C-Tail
Plasmid#237514PurposeProtoplast expressionDepositorInsertRwt4Ca+C-Tail
UseTagsExpressionPlantMutationAdd C-terminal extensionPromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)
Plasmid#228253PurposepU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)DepositorInsertmpknot entry plasmid backbone for epegRNA expression via human U6 promoter
UseTagsExpressionMammalianMutationn/aPromoterU6Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-Bs3-sgRNA (BsaI)
Plasmid#188280PurposeMammalian expression, Genome editingDepositorInsertBs3-sgRNA
UseCRISPRTagsExpressionMammalianMutationnonePromoterU6Available sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-3'
Plasmid#110406PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2DepositorInsertGLS glutaminase (GLS Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only