We narrowed to 4,505 results for: pcr

1. pCRISPaint-ProteoTuner DD

   Plasmid

   #67181

   Purpose

   CRISPaint universal donor for fusion tagging allowing inducible destabilization

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   ProteoTuner DD
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR-S12

   Plasmid

   #84031

   Purpose

   To episomally express codon optimized Cas9 and chimeric guide RNA

   Depositor

   Sen Wu

   Article

   Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

   Inserts

   hSpCas9
   eGFP
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and CMV (downstream of F2A self-cleaving pept…

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR eGFPCreERT2 L1-R5

   Plasmid

   #43961

   Depositor

   Peter Hohenstein

   Article

   Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

   Insert

   eGFP-CreERT2
   

   Use

   Multisite gateway entr clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-TagGFP2

   Plasmid

   #67166

   Purpose

   CRISPaint universal donor for TagGFP2 fusion tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   TagGFP2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISPaint-NanoLuc

   Plasmid

   #67178

   Purpose

   CRISPaint universal donor for NanoLuc fusion tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   NanoLuc
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

   Plasmid

   #127177

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   Flag- HA- Tandem Epitopes

   Expression

   Mutation

   Promoter

   None

   Available since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRainbow-DONOR1

   Plasmid

   #75398

   Purpose

   CRISPRainbow-DONOR1

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   Cm(R)-CcdB
   

   Use

   CRISPR

   Tags

   no

   Expression

   Mutation

   Promoter

   Bacteria

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR3.1-Luc

   Plasmid

   #106457

   Purpose

   luciferase reporter, CMV promoter

   Depositor

   Georg Weber

   Article

   He et al Biochem Biophys Res Commun. 2004 Aug 13;

   Insert

   luciferase reporter, CMV promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   WT promoter

   Promoter

   Available since

   Nov. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCR-HA-IKKbeta

   Plasmid

   #15470

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase beta (Ikbkb Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pCRISPaint-TagBFP

    Plasmid

    #67168

    Purpose

    CRISPaint universal donor for TagBFP fusion tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    TagBFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. shha-pCRII

    Plasmid

    #68962

    Purpose

    for antisense probe, NotI w/ Sp6

    Depositor

    David Traver

    Article

    Clements et al Nature. 2011 Jun 8;474(7350):220-4. doi:

    Insert

    shha (shha Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCR8/GW_TOPO_Cdk12_Untagged

    Plasmid

    #127176

    Purpose

    Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    None

    Expression

    Mutation

    Promoter

    None

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPR_full-site

    Plasmid

    #186277

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRII p15

    Plasmid

    #16454

    Depositor

    Bert Vogelstein

    Article

    Jen et al Cancer Res. 1994 Dec 15. 54(24):6353-8.

    Insert

    p15 (CDKN2B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pCri-18a

    Plasmid

    #61326

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pAC1817_pCR8-gSMN2-EX

    Plasmid

    #118651

    Purpose

    Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-EX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR8-PHF19

    Plasmid

    #68864

    Purpose

    GW entry vector for PHF19

    Depositor

    Adrian Bracken

    Article

    Brien et al Nat Struct Mol Biol. 2012 Dec;19(12):127

    Insert

    PHF19 (PHF19 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCRISPReporter-mCherry

    Plasmid

    #65008

    Purpose

    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    User-defined

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRU5-inGFPt

    Plasmid

    #60236

    Purpose

    HTLV-1 based transfer vector encoding gfp-turbo reporter gene interrupted by intron

    Depositor

    Dmitriy Mazurov

    Article

    Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

    Insert

    inGFPt
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-SBP

    Plasmid

    #67173

    Purpose

    CRISPaint universal donor for streptavidin binding peptide fusion tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    SBP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCRU5HT1-inLuc

    Plasmid

    #58954

    Purpose

    HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

    Depositor

    David Derse, Dmitriy Mazurov

    Article

    Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

    Insert

    inLuc
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CMV/R/U5

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCR3.1-OPNc

    Plasmid

    #107982

    Purpose

    expression of OPN splice variant c

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    osteopontin splice variant c (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    splice variant

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCRISPaint-3xFlag

    Plasmid

    #67170

    Purpose

    CRISPaint universal donor for flag fusion tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    3xFlag
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCR2.1-myco

    Plasmid

    #36870

    Depositor

    Mario Chin

    Insert

    myco
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCR3.1-cGK1

    Plasmid

    #106452

    Purpose

    expression of murine cyclic GMP-dependent kinase

    Depositor

    Georg Weber

    Article

    He et al Biochem Biophys Res Commun. 2004 Aug 13;

    Insert

    cyclic GMP-dependent kinase (Prkg1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRISPomyces-Sth1Cas9

    Plasmid

    #129552

    Purpose

    Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

    Depositor

    Mingzi M. Zhang, Fong Tian Wong

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    Sth1Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CaV2.1 (pCR3.1)

    Plasmid

    #26573

    Depositor

    Diane Lipscombe

    Article

    Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

    Insert

    Cacna1a (Cacna1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

28. pCR3.1-OPNb

    Plasmid

    #107981

    Purpose

    expression of OPN splice variant b

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    osteopontin splice variant b (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    splice variant

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRU5-inmCherry

    Plasmid

    #60238

    Purpose

    HTLV-1 based transfer vector encoding mcherry reporter gene interrupted by intron

    Depositor

    Dmitriy Mazurov

    Article

    Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

    Insert

    inmCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCR8 PALI1

    Plasmid

    #114442

    Purpose

    Entry vector for gateway cloning containing human PALI1 coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    PALI1 (LCOR Human)
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge