2,172 results

Showing: 181 - 210 of 2172 results

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p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

Plasmid

#227774

Purpose

Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

loxP-dsR-loxP

Use

CRISPR

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

Plasmid

#105127

Purpose

Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein

Depositor

Kimberley Evason, Didier Stainier

Article

Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d

Inserts

Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)

Use

Zebrafish transgenesis

Promoter

cryaa (crystallin) and fabp10a

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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I-SceI pB 5UAS::ZfSyGCaMP2

Plasmid

#26125

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (sypb Zebrafish)

Use

Zebrafish expression

Tags

GCaMP2

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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p5e irg1

Plasmid

#188698

Purpose

Gateway promoter element for the zebrafish irg1 gene

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

irg1 (acod1 Zebrafish)

Use

Synthetic Biology

Expression

Mammalian

Promoter

irg1

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RNAseH1

Plasmid

#65782

Purpose

expresses FLAGHA-tagged RNAsH1 in mammalian cells

Depositor

Thomas Tuschl

Insert

RNaseH1 (RNASEH1 Human, Zebrafish)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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acta1-Cavin4a-Clover

Plasmid

#163853

Purpose

Zebrafish muscle specific expression of Cavin4a-Clover, Parton lab clone DPV

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin4a (cavin4a Zebrafish)

Use

Zebrafish expression

Tags

Clover

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-Rac1

Plasmid

#109581

Purpose

Multisite gateway vector for 3' tagging with Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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5UAS zfSynaptophysin:GFP

Plasmid

#74316

Purpose

A fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeats

Depositor

Martin Meyer, Stephen Smith

Article

Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

Insert

synaptophysin

Tags

EGFP

Mutation

Q12H and deletion of F13 (please see depositor co…

Promoter

5UAS

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pT3TS-Cavin4b-TurboID

Plasmid

#163851

Purpose

In vitro transcription of Cavin4b-TurboID. Parton lab clone JTA

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin4b (cavin4b Zebrafish)

Use

In vitro transcription of rna

Tags

TurboID

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-CD44b-TurboID

Plasmid

#163850

Purpose

In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

CD44b (cd44b Zebrafish)

Use

In vitro transcription of rna

Tags

TurboID

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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p5E mpeg1

Plasmid

#135199

Purpose

Macrophage specific label

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

mpeg1 promoter

Use

Synthetic Biology

Promoter

mpeg1

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pME-Trpa1b-2A-mCherry

Plasmid

#106291

Purpose

Gateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherry

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Insert

Trpa1b (trpa1b Zebrafish)

Use

Gateway middle entry

Tags

2A-mCherry

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-CAAX-SV40

Plasmid

#117809

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-mito-Dendra2

Plasmid

#107590

Purpose

Expresses mitochondria localized Dendra2 in zebrafish neutrophils

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

mito-Dendra2

Tags

mitochondira localization sequence

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-lyn-GFP

Plasmid

#35037

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

Lyn-GFP (lyn Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mammalian

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
p5E-smyhc1

Plasmid

#126748

Purpose

Zebrafish slow myosin heavy chain promoter. Clone 667

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

smyhc1 (smyhc1 Zebrafish)

Use

Multisite gateway entry clone

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rac1

Plasmid

#109529

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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mpeg:lamp2-mCherry-stop; cmlc2:GFP

Plasmid

#213740

Purpose

Expresses Lamp2-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

lamp2-mCherry

Use

Zebrafish

Promoter

mpeg1.1

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rac1-DN

Plasmid

#109530

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3e UbB polyA

Plasmid

#188702

Purpose

Gateway 3' element encoding the ubiquitin B polyadenylation sequence

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

UbB polyA (ubb Zebrafish)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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zCREST3:redCas9; control_gRNA

Plasmid

#199334

Purpose

pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
control sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk18

Plasmid

#209000

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk18 (kcnk18 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:BRAF

Plasmid

#118846

Purpose

Expresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

BRAF V600E (BRAF Human, Zebrafish)

Use

Zebrafish expression, tol2

Mutation

BRAF V600E

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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p5E-elavl3

Plasmid

#72640

Purpose

Gateway p5E 5'entry clone with elavl3 (HuC) enhancer/promoter for pan-neuronal expression in zebrafish

Depositor

Joshua Bonkowsky

Insert

elavl3 enhancer (elavl3 Zebrafish)

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-dr-myl7-pro (JDW 1148)

Plasmid

#229836

Purpose

A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

cmlc2/myl7

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-0.349cldn15la

Plasmid

#125026

Purpose

Five prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.

Depositor

John Rawls

Article

Murdoch et al PLoS Pathog. 2019 Mar 7;15(3):e1007381.

Insert

Claudin 15-like a (cldn15la Zebrafish)

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP1-sox17 promoter

Plasmid

#31400

Depositor

Didier Stainier

Insert

sox17 promoter (sox17 Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
pME Integrin beta 1b

Plasmid

#105976

Purpose

entry clone for gateway cloning, Full length Integrin beta 1b without stop

Depositor

Caren Norden

Article

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Integrinbeta1b

Expression

Bacterial

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 2kb promoter/AcGFP

Plasmid

#98099

Purpose

Zebrafish aBb crystallin 2kb promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA(tyr)

Plasmid

#64250

Purpose

expresses sgRNA(tyr) under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

U6a:sgRNA (tyr)

Use

CRISPR

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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2,172 results