Skip to main content
Addgene
Showing: 241 - 280 of 599 results

  1. pSY45

    Plasmid
    #48355
    Purpose
    Targets TbMCM-BP for Cre-lox mediated knockout in T. brucei
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    loxP-SAS-HYG-Ty1-TK-loxP flanked by TbMCM-BP-targeting homologies
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48355-plasmid-map-sequence-id-70032

    Enlarge

  3. pHJ32

    Plasmid
    #48361
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48361-plasmid-map-sequence-id-70052

    Enlarge

  4. pHJ33

    Plasmid
    #48362
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48362-plasmid-map-sequence-id-70053

    Enlarge

  5. pHJ17

    Plasmid
    #48356
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48356-plasmid-map-sequence-id-70042

    Enlarge

  6. pHJ22

    Plasmid
    #48358
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Neomycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-NEO-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48358-plasmid-map-sequence-id-70047

    Enlarge

  7. pHJ18

    Plasmid
    #48357
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48357-plasmid-map-sequence-id-70046

    Enlarge

  8. pHJ41

    Plasmid
    #48360
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48360-plasmid-map-sequence-id-70051

    Enlarge

  9. pHJ42

    Plasmid
    #48359
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Bleomycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BLE-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48359-plasmid-map-sequence-id-70049

    Enlarge

  11. HS_NAM-B1

    Plasmid
    #154064
    Purpose
    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    HvHSP17, OsAct, and ZmUbi
    Available since
    Sept. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154064-plasmid-map-sequence-id-313309

    Enlarge

  15. pNM3749

    Plasmid
    #154108
    Purpose
    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector
    Depositor
    Michael Nonet
    Article
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Inserts
    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    Use
    Cre/Lox; Rmce
    Tags
    Expression
    Worm
    Mutation
    Promoter
    not applicable
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154108-plasmid-map-sequence-id-306067

    Enlarge

  16. pFloxed DHFR-TS*

    Plasmid
    #100605
    Purpose
    pUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTR
    Depositor
    David Sibley
    Insert
    Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100605-plasmid-map-sequence-id-192861

    Enlarge

  18. pNM3746

    Plasmid
    #154109
    Purpose
    loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector
    Depositor
    Michael Nonet
    Article
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Inserts
    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::His-58::FRT3
    mex-5p::FLP D5::sl2::mNG::glh2 3'
    Use
    Cre/Lox; Rmce
    Tags
    Expression
    Worm
    Mutation
    Promoter
    not applicable
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154109-plasmid-map-sequence-id-306068

    Enlarge

  19. pNB1-LOX

    Plasmid
    #154058
    Purpose
    Level 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    LoxP-flanked GUS and TtNAM-B1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    ZmUbi
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154058-plasmid-map-sequence-id-313109

    Enlarge

  20. pGEM-floxedEGFP-TRI2A

    Plasmid
    #74669
    Purpose
    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
    Depositor
    Roberto Giovannoni
    Insert
    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    pCAGGS
    Available since
    Feb. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    74669-plasmid-map-sequence-id-147558

    Enlarge

  22. pHD-DsRed-w+

    Plasmid
    #80926
    Purpose
    Vector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    LoxP-3xP3-DsRed-LoxP
    GMR-white
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and GMR::hsp70
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80926-plasmid-map-sequence-id-152782

    Enlarge

  24. pUb-tdTomato-LP-Neo-LP

    Plasmid
    #231509
    Purpose
    Fluorescent reporter for tdTomato expression in Acanthamoeba via the Cre/loxP system
    Depositor
    Yeonchul Hong
    Insert
    tdTomato fluorescent protein
    Use
    Unspecified; Acanthamoeba castellanii neff strain
    Tags
    Expression
    Mutation
    Promoter
    Acanthamoeba polyubiquitin
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231509-plasmid-map-sequence-id-462659

    Enlarge

  27. pHD-DsRed-attP-w+

    Plasmid
    #80898
    Purpose
    Vector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    GMR-white
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and GMR::hsp70
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    80898-plasmid-map-sequence-id-172486

    Enlarge

  30. pB6-Hipp11-tdTomato cre reporter-FNF-DTA

    Plasmid
    #183024
    Purpose
    B6J Hipp11-pCAG-loxP-FNF-bGHpA-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183024-plasmid-map-sequence-id-360499

    Enlarge

  31. pPNT6-eGFP

    Plasmid
    #71303
    Purpose
    This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression
    Depositor
    Gian Luca Forni, Marco Musso
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    71303-plasmid-map-sequence-id-130367

    Enlarge

  32. pB6-Hipp11-td-sfGFP-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183026
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-td-sfGFP-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183026-plasmid-map-sequence-id-360495

    Enlarge

  33. pB6-Hipp11-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183025
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183025-plasmid-map-sequence-id-360497

    Enlarge

Showing: 241 - 280 of 599 results