Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,793 results for: plasmid for e coli

Clear keywords
Showing: 241 - 280 of 1793 results

  1. pGBW-m4046897

    Plasmid
    #145579
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145579-plasmid-map-sequence-id-353415

    Enlarge

  2. pGBW-m4046761

    Plasmid
    #145582
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp11
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145582-plasmid-map-sequence-id-287759

    Enlarge

  3. pGBW-m4046777

    Plasmid
    #145583
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145583-plasmid-map-sequence-id-287760

    Enlarge

  4. pGBW-m4046826

    Plasmid
    #145687
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145687-plasmid-map-sequence-id-292489

    Enlarge

  5. pInt_attP1_LCS_kanR

    Plasmid
    #205300
    Purpose
    Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205300-plasmid-map-sequence-id-407004

    Enlarge

  7. pHelper_NoMutL_V2_gentR

    Plasmid
    #205293
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    205293-plasmid-map-sequence-id-410127

    Enlarge

  8. pHelper_Ec1_V1_gentR

    Plasmid
    #205291
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Available Since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205291-plasmid-map-sequence-id-405409

    Enlarge

  9. pHelper_Ec1_V1_ampR

    Plasmid
    #205292
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205292-plasmid-map-sequence-id-404792

    Enlarge

  11. pGEX4T1-Opt WWP1

    Plasmid
    #179327
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1
    Depositor
    Arthur Haas
    Insert
    WWP1 (WWP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Available Since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179327-plasmid-map-sequence-id-358706

    Enlarge

  12. pGEX4T1-WWP1F792D

    Plasmid
    #179328
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1 F792D
    Depositor
    Arthur Haas
    Insert
    WWP1 F792D (WWP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    F792D mutation; Codon Optimized for expression in…
    Available Since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179328-plasmid-map-sequence-id-358707

    Enlarge

  13. StarShip01

    Plasmid
    #164505
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Availability
    Academic Institutions and Nonprofits only
    164505-plasmid-map-sequence-id-324722

    Enlarge

  14. pGBW-m4046887

    Plasmid
    #145730
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145730-plasmid-map-sequence-id-292497

    Enlarge

  15. pGBW-m4136464

    Plasmid
    #149534
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149534-plasmid-map-sequence-id-307562

    Enlarge

  16. pGBW-m4136461

    Plasmid
    #149538
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149538-plasmid-map-sequence-id-307561

    Enlarge

  17. pGBW-m4046758

    Plasmid
    #145670
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145670-plasmid-map-sequence-id-297837

    Enlarge

  18. pGBW-m4046801

    Plasmid
    #145741
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145741-plasmid-map-sequence-id-298377

    Enlarge

  19. pGBW-m4136454

    Plasmid
    #149529
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149529-plasmid-map-sequence-id-297077

    Enlarge

  20. pGBW-m4046931

    Plasmid
    #145706
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145706-plasmid-map-sequence-id-307566

    Enlarge

  21. pGBW-m4136462

    Plasmid
    #149533
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149533-plasmid-map-sequence-id-307560

    Enlarge

  22. pGBW-m4046863

    Plasmid
    #145682
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145682-plasmid-map-sequence-id-298370

    Enlarge

  23. pGBW-m4136457

    Plasmid
    #149537
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149537-plasmid-map-sequence-id-314686

    Enlarge

  24. pUC57-BBclone1

    Plasmid
    #137069
    Purpose
    E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Inserts
    BB0405 (BB_0405 Borrelia burgdorferi B31)
    BB0406
    P66
    BBA57
    HtrA
    RevA (revA Borrelia burgdorferi B31)
    BB0238
    Expression
    Bacterial
    Mutation
    wild-type, full-length (signal-peptide-containing…
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137069-plasmid-map-sequence-id-275695

    Enlarge

  25. pBEL1296

    Plasmid
    #253834
    Purpose
    Expression plasmid of soluble PqiC(delta1-16)
    Depositor
    Gira Bhabha, Damian Ekiert
    Insert
    pqiC
    Tags
    His-PqiC
    Expression
    Bacterial
    Mutation
    PqiC delta1-16
    Available Since
    May 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    253834-plasmid-map-sequence-id-510943

    Enlarge

  26. pBEL3442

    Plasmid
    #253843
    Purpose
    Expression plasmid of PqiAB(P541G)C
    Depositor
    Gira Bhabha, Damian Ekiert
    Insert
    pqiABC
    Tags
    PqiA-(His-PqiB)-PqiC
    Expression
    Bacterial
    Mutation
    PqiB P541G
    Available Since
    May 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    253843-plasmid-map-sequence-id-510942

    Enlarge

  27. pBEL3441

    Plasmid
    #253842
    Purpose
    Expression plasmid of PqiAB(P539G)C
    Depositor
    Gira Bhabha, Damian Ekiert
    Insert
    pqiABC
    Tags
    PqiA-(His-PqiB)-PqiC
    Expression
    Bacterial
    Mutation
    PqiB P539G
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    253842-plasmid-map-sequence-id-505703

    Enlarge

  28. pBEL3444

    Plasmid
    #253844
    Purpose
    Expression plasmid of PqiABC(Y64K)
    Depositor
    Gira Bhabha, Damian Ekiert
    Insert
    pqiABC
    Tags
    PqiA-(His-PqiB)-PqiC
    Expression
    Bacterial
    Mutation
    PqiC Y64K
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    253844-plasmid-map-sequence-id-505699

    Enlarge

  29. pBEL3440

    Plasmid
    #253841
    Purpose
    Expression plasmid of PqiAB(D538G)C
    Depositor
    Gira Bhabha, Damian Ekiert
    Insert
    pqiaBC
    Tags
    PqiA-(His-PqiB)-PqiC
    Expression
    Bacterial
    Mutation
    PqiB D538G
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    253841-plasmid-map-sequence-id-505697

    Enlarge

  30. pBEL3445

    Plasmid
    #253845
    Purpose
    Expression plasmid of PqiABC(Y161K)
    Depositor
    Gira Bhabha, Damian Ekiert
    Insert
    pqiABC
    Tags
    PqiA-(His-PqiB)-PqiC
    Expression
    Bacterial
    Mutation
    PqiC Y161K
    Available Since
    April 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    253845-plasmid-map-sequence-id-505365

    Enlarge

  31. pBAD02-EcPD-T4-8

    Plasmid
    #254567
    Purpose
    For the expression of the PD-T4-8 system encoded by Escherichia coli MOD1-ECOR31
    Depositor
    Philip Kranzusch
    Insert
    EcPD(D/E)XK nuclease
    Expression
    Bacterial
    Available Since
    May 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    254567-plasmid-map-sequence-id-508547

    Enlarge

  32. pBAD02-EcPD-T4-1

    Plasmid
    #254565
    Purpose
    For the expression of the PD-T4-1 system encoded by Escherichia coli strain UMB0934
    Depositor
    Philip Kranzusch
    Insert
    EcPD(D/E)XK nuclease
    Expression
    Bacterial
    Available Since
    May 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    254565-plasmid-map-sequence-id-508545

    Enlarge

  33. pHL1914

    Plasmid
    #53042
    Purpose
    PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR
    Depositor
    Han Lim
    Inserts
    GFP
    tetR
    Use
    Synthetic Biology
    Tags
    RBS(st3) and hfqlead
    Expression
    Bacterial
    Promoter
    PconNoHindM2 and pLlacO-1
    Available Since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pBEL3439

    Plasmid
    #253840
    Purpose
    Expression plasmid of PqiAB(delta531-546)C
    Depositor
    Gira Bhabha, Damian Ekiert
    Insert
    pqiABC
    Tags
    PqiA-(His-PqiB)-PqiC
    Expression
    Bacterial
    Mutation
    PqiB delta531-546
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    253840-plasmid-map-sequence-id-505705

    Enlarge

  36. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

    Enlarge

  38. pTJK368

    Plasmid
    #138006
    Purpose
    BirA* expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Tami Kingsbury
    Insert
    BirA*
    Use
    Lentiviral
    Mutation
    None
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138006-plasmid-map-sequence-id-275000

    Enlarge

  39. StarShip02

    Plasmid
    #164506
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Availability
    Academic Institutions and Nonprofits only
    164506-plasmid-map-sequence-id-324723

    Enlarge

  40. StarShip03

    Plasmid
    #164507
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Availability
    Academic Institutions and Nonprofits only
    164507-plasmid-map-sequence-id-324724

    Enlarge

Showing: 241 - 280 of 1793 results