We narrowed to 2,256 results for: D10

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pGEX-parkin D106A

Plasmid

#227362

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin D106A (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 D101R

Plasmid

#224349

Purpose

Expresses human KDAC8 (HDAC8) D101R in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to arginine

Promoter

T5

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress401-KDAC8 D101A

Plasmid

#224347

Purpose

Expresses human KDAC8 (HDAC8) D101A in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to alanine

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Salsa-BC238-P3D10

Plasmid

#203079

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC238

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC238

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC142-P2D10

Plasmid

#202983

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC142

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC142

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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mito-PDCD10-mScarlet-I

Plasmid

#208887

Purpose

Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM3 (PDCD10 Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

PDCD10 targeted to the mitonchondria

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC46-P1D10

Plasmid

#202887

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC46

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC46

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

Plasmid

#199253

Purpose

Expression construct encoding the high specificity SpCas9-KA-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity S. pyogenes Cas9-D10A-KA nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 D10-25-msfGFP

Plasmid

#180334

Purpose

mammalian expression of human SEPT9_i1 D10-25 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 D10-25

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag

Plasmid

#129255

Purpose

Express Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST

Plasmid

#129260

Purpose

Express soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag-ST

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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phSOD1-D101G-N’His

Plasmid

#117704

Purpose

mutation on stability/solubility of SOD1

Depositor

Elizabeth Fisher

Insert

SOD1 (SOD1 Human)

Tags

His tag with Thrombin cleavage site

Expression

Bacterial

Mutation

D101G

Promoter

T7

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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KIF4A D10.2 gRNA

Plasmid

#90725

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF4A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF4A (Guide Designation D10.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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NUDC D10.4 gRNA

Plasmid

#90807

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUDC

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NUDC (Guide Designation D10.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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LSM4 D10.3 gRNA

Plasmid

#90737

Purpose

3rd generation lentiviral gRNA plasmid targeting human LSM4

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

LSM4 (Guide Designation D10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pX335A_hCas9(D10A)_IRF8gRNA4

Plasmid

#89722

Purpose

Knockout IRF8 in human cells

Depositor

Martin Zenke

Article

Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

Insert

IRF8 gRNA4 (IRF8 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pX335A_hCas9(D10A)_IRF8gRNA3

Plasmid

#89721

Purpose

Knockout IRF8 in human cells

Depositor

Martin Zenke

Article

Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

Insert

IRF8 gRNA3 (IRF8 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAD100-CHIP-myc-His

Plasmid

#236054

Purpose

Mammalian expression of human CHIP with a C-terminal myc-His tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

CHIP (STUB1 Human)

Tags

myc-His

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pKMD106h - intein-Cas9(C574)

Plasmid

#64191

Purpose

Expresses intein-Cas9(C574) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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hRad51(A89E)–Cas9(D10A)

Plasmid

#125563

Purpose

Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(A89E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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