We narrowed to 16,834 results for: Emb;

1. pD649-HAsp-EMB-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156680

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   EMB (EMB Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUPD2 Assembly Register 3/3 Part - 5'UTR:Luc:Tnos (GB1500)

   Plasmid

   #160581

   Purpose

   Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of Luc and NOS terminator

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   Luc
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5 FLAG membrane-bound lipin-1 (delta200)

   Plasmid

   #120278

   Purpose

   Expression of ER/Golgi-tethered lipin-1 in mammalian cells

   Depositor

   Sirio Dupont

   Article

   Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

   Insert

   Lipin-1 (Lpin1 Mouse)
   

   Use

   Tags

   FLAG and in-frame fusion with the delta200 Golgi-…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Plasmid

   #163043

   Purpose

   QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   QuasAr2
   

   Use

   Lentiviral

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

   Plasmid

   #163063

   Purpose

   Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   CheRiff
   

   Use

   Gateway entry vector

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

   Plasmid

   #158585

   Purpose

   Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

   Depositor

   Cynthia Kenyon

   Article

   Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

   Insert

   membrane localization signal-tagBFP-wrmScarlet11
   

   Use

   Tags

   tagBFP

   Expression

   Yeast

   Mutation

   GGGS linker

   Promoter

   TEF

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Plasmid

   #163062

   Purpose

   Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   QuasAr2
   

   Use

   Gateway entry vector

   Tags

   mOrange2

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX Supt6h-SH2

   Plasmid

   #46518

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Supt6h (SUPT6H Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1317-1441

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-hCRM1

   Plasmid

   #17647

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   CRM1 (XPO1 Human)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-Expotin 1

    Plasmid

    #42047

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Exportin-1 (XPO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET29b Tau Cys

    Plasmid

    #108867

    Purpose

    Expresses human Tau with a mutated cysteine

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I260C C291A C322A to allow maleimide dye labelling

    Promoter

    T7

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. His8-TEV-PseC

    Plasmid

    #89724

    Purpose

    Expresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage site

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    PseC (pseC Campylobacter jejuni)
    

    Use

    Tags

    His8 and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

13. His8-TEV-PseH

    Plasmid

    #89725

    Purpose

    Expresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage site

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    PseH (pseH Campylobacter jejuni)
    

    Use

    Tags

    2xHis Gln 5xHis and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pISH-Pou6f1

    Plasmid

    #74356

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pou6f1. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    POU domain, class 6, transcription factor 1 (Pou6f1 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMT-mMyh3CDS

    Plasmid

    #192843

    Purpose

    Plasmid to produce ISH probe for mMyh3 gene with CDS sequence

    Depositor

    Masafumi Inui

    Article

    Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

    Insert

    myosin heavy chain 3 (Myh3 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMT-mMyh3UTR

    Plasmid

    #192844

    Purpose

    Plasmid to produce ISH probe for mMyh3 gene with UTR sequence

    Depositor

    Masafumi Inui

    Article

    Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

    Insert

    myosin heavy chain 3 (Myh3 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAPM-miR30-XPO1-ts1

    Plasmid

    #174286

    Purpose

    XPO1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    XPO1 shRNA (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAPM-miR30-XPO1-ts2

    Plasmid

    #174287

    Purpose

    XPO1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    XPO1 shRNA (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAPM-miR30-XPO1-ts3

    Plasmid

    #174288

    Purpose

    XPO1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    XPO1 shRNA (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SSB-wt

    Plasmid

    #197260

    Purpose

    wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

    Depositor

    Timothy Lohman

    Article

    Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

    Insert

    wt E. coli SSB
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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