We narrowed to 7,149 results for: CIT

1. pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

   Plasmid

   #158963

   Purpose

   Cre-dependet Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Kesavan et al Development. 2020 Jun 8;147(11). pii: de

   Inserts

   hsp70l promoter
   floxed Non-FP cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAsCpf1(TYCV)(BB) (pY211)

   Plasmid

   #89352

   Purpose

   Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CBh

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-His-VCre

   Plasmid

   #123131

   Purpose

   Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   VCre recombinase
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. xCas9(3.6)-BE4

   Plasmid

   #108386

   Purpose

   Mammalian expression vector for xCas9(3.6)-BE4

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.6)-BE4
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK2301

   Plasmid

   #65778

   Purpose

   Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   St1Cas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-mEGFP(A206K)-paCaMKII(K42M)

   Plasmid

   #165440

   Purpose

   Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(K42M)
   

   Use

   Tags

   mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   K42M: Kinase dead mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. xCas9(3.6)-BE3

   Plasmid

   #108385

   Purpose

   Mammalian expression vector for xCas9(3.6)-BE3

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.6)-BE3
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH3626

   Plasmid

   #179630

   Purpose

   Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   RFP

   Expression

   Worm

   Mutation

   Promoter

   Pacr-2(s)

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

   Plasmid

   #89570

   Purpose

   Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

   Depositor

   McLean Bolton

   Article

   Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

   Inserts

   Channelrhodopsin
   Histone 2B
   

   Use

   AAV

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   synapsin

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-RhoB Sensor-IRES-Blast

    Plasmid

    #222633

    Purpose

    Rho reporter

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    RhoB Sensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CRT-GFP pcDNA3.1

    Plasmid

    #80978

    Purpose

    mammalian expression of CRT-GFP

    Depositor

    Peter Steyger

    Article

    Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

    Insert

    CRT (Calr Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    no KDEL

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

12. px552-sg-gria1-HT-SEP

    Plasmid

    #187653

    Purpose

    Contains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.

    Depositor

    Erin O'Shea

    Article

    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

    Insert

    HaloTag-SEP donor
    

    Use

    AAV and CRISPR

    Tags

    N/A

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-Chronos-mT-Sapphire-NRN

    Plasmid

    #149692

    Purpose

    For packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAV

    Depositor

    Robert Malenka

    Article

    Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

    Insert

    Chronos
    

    Use

    AAV

    Tags

    mT-Sapphire

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSP977

    Plasmid

    #65774

    Purpose

    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E mutation in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-InPAkt

    Plasmid

    #181914

    Purpose

    Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

    Insert

    InPAkt
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-SENP8 (full length))

    Plasmid

    #16361

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP8 full length (SENP8 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    human SENP8 full length, see Depositor Comments

    Promoter

    Available since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB1H1-Zif268

    Plasmid

    #12612

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Zif268 in pB1H1 (Egr1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. SB51

    Plasmid

    #48184

    Purpose

    Express full length HA tagged p35sr in mammalian cells

    Depositor

    Shoumo Bhattacharya

    Article

    Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pMCSG28-SH3-588

    Plasmid

    #185920

    Purpose

    Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

    Depositor

    Brian Kuhlman

    Article

    Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

    Insert

    SH3-588
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Designed enzyme with novel interface between huma…

    Promoter

    T7

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-ChrimsonR-LSSmOrange-NRN

    Plasmid

    #149693

    Purpose

    For packaging and expression of ChrimsonR-LSSmOrange featuring a neuritin (NRN) 3' UTR in neurons via AAV

    Depositor

    Robert Malenka

    Article

    Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-CAG-miniSOG

    Plasmid

    #193913

    Purpose

    Control plasmid that expresses non-targeted miniSOG in mammalian cells

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pETNKI-his-SUMO-RNF168ring (1-189)

    Plasmid

    #63570

    Purpose

    Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    6xHis and SUMO3

    Expression

    Bacterial

    Mutation

    Ring domain only (residues 1-189)

    Promoter

    T7

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pHH0103_SNX18-1/1

    Plasmid

    #91369

    Purpose

    Protein expression and purification of human SH3 domain construct SNX18-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SNX18-1/1 (SNX18 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHH0103_TNK2-1/1

    Plasmid

    #91377

    Purpose

    Protein expression and purification of human SH3 domain construct TNK2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TNK2-1/1 (TNK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-SENP5 (catalytic domain)

    Plasmid

    #16358

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP5 catalytic domain (SENP5 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 567 to 755 catalytic domain of human S…

    Promoter

    Available since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pENTR_hEMSY

    Plasmid

    #125156

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    EMSY (EMSY Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRS303 Gal TDP-43 YFP

    Plasmid

    #27468

    Depositor

    Aaron Gitler

    Article

    Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    D23A mutation in TDP-43 sequence

    Promoter

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pAAV-EF1α-DIO-SN

    Plasmid

    #181741

    Purpose

    AAV vector for expressing SN

    Depositor

    Yasunori Hayashi

    Article

    Goto et al Science. 2021 Nov 12;374(6569):857-863.

    Insert

    SuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEPOR1CB0013

    Plasmid

    #117549

    Purpose

    Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p40phox-PX (1-144)

    Plasmid

    #119123

    Purpose

    Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    p40phox-PX (1-144)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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