8,723 results

1. TagBFP2-CAAX

   Plasmid

   #116856

   Purpose

   plasma membrane marker

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   HRAS
   

   Use

   Tags

   TagBFP2

   Expression

   Mammalian

   Mutation

   Amino acids 172-189

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. EJ2GFP-puro

   Plasmid

   #44025

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

   Insert

   EJ2GFP egfp-based chromosomal break reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   insertion of I-SceI, 3 frame stop, and flanking m…

   Promoter

   pCAGGS

   Available since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Dcp1a

   Plasmid

   #153972

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1a

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA decapping enzyme 1a (DCP1A Human)
   

   Use

   Tags

   pAcGFP-C1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Neo-Bam p53 R175H

   Plasmid

   #16436

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 R175H (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R175H

   Promoter

   CMV

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Ub KO

   Plasmid

   #11934

   Depositor

   Nico Dantuma

   Article

   Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

   Insert

   Ubiquitin KO (RBX1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   All 7 lysines of ubiquitin mutated to arginines.

   Promoter

   Available since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. scFLARE2

   Plasmid

   #158700

   Purpose

   Neuronal expression of scFLARE2 component

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   scFLARE2
   

   Use

   AAV

   Tags

   HA, Flag, VP16

   Expression

   Mammalian

   Mutation

   Truncated form of TEV protease (220-242 amino aci…

   Promoter

   Syn

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG

   Plasmid

   #105543

   Purpose

   AAV expression of EGFP from cTNT promoter

   Depositor

   James M. Wilson

   Has Service

   AAV9

   Insert

   cTNT-PI-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cTNT

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. murine TCR OTI-2A.pMIG II

   Plasmid

   #52111

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   T2A (from Thosea asigna virus)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-mitoLbNOX

   Plasmid

   #74448

   Purpose

   This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   mitoLbNOX
   

   Use

   Tags

   Flag tag with linker and mitochondrial targeting …

   Expression

   Mutation

   Human codon optimized

   Promoter

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX iLOV

    Plasmid

    #26587

    Depositor

    John Christie

    Article

    Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

    Insert

    iLOV
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C2-NLRP3

    Plasmid

    #73955

    Purpose

    Express EGFP-tagged human NLRP3 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCRISPR-tagBFP

    Plasmid

    #75160

    Purpose

    Derived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistance

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK292 (mAID-mCherry2-NeoR)

    Plasmid

    #72830

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Neo
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. paavCAG-iCre

    Plasmid

    #51904

    Purpose

    For AAV expression of iCre recombinase

    Depositor

    Jinhyun Kim

    Article

    Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

    Insert

    improved Cre recombinase
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-GCaMP6s

    Plasmid

    #40753

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6s
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRubiG-T2A-Cas9

    Plasmid

    #75348

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Matrix-roGFP

    Plasmid

    #49437

    Purpose

    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

    Depositor

    Paul Schumacker

    Article

    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

    Insert

    Matrix-roGFP2
    

    Use

    Adenoviral

    Tags

    Cytochrome c oxidase subunit IV mitochondrial tar…

    Expression

    Mammalian

    Mutation

    C48S, Q80R, S147C, and Q204C in Clontech's G…

    Promoter

    CMV

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3-GFP

    Plasmid

    #74165

    Purpose

    Mammalian expression of EGFP

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

    Plasmid

    #167582

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge