2,226 results

1. pORANGE Cacnb3-GFP KI

   Plasmid

   #139662

   Purpose

   Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE Cacnb4-GFP KI

   Plasmid

   #139663

   Purpose

   Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-miniSOG-RatLC3

   Plasmid

   #55095

   Purpose

   Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   LC3
   

   Use

   Tags

   mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. Geph UTR shRNA

   Plasmid

   #121070

   Purpose

   shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNA

   Depositor

   Angel de Blas

   Article

   Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

   Insert

   GPHN shRNA (Gphn Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. peGFP-N2-rPrmp

   Plasmid

   #22735

   Depositor

   Lynn Hudson

   Article

   Nielsen et al J Neurosci. 2006 Sep 27. 26(39):9881-91.

   Insert

   Proline-rich transmembrane protein
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. Jag1ICD-HA pBacPAK8-3

   Plasmid

   #17331

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

   Insert

   Jagged1 (Jag1 Rat)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pR2Q306c

   Plasmid

   #101027

   Purpose

   fluorescent, functional, glutamate receptor subunit

   Depositor

   Thomas Hughes

   Article

   Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

   Insert

   Glutamate receptor subunit (Gria2 Rat)
   

   Use

   Tags

   fluorescent protein

   Expression

   Mammalian

   Mutation

   tagged with fluorescent protein

   Promoter

   CMV

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rat SERCA2b (pMT2)

   Plasmid

   #75184

   Purpose

   Mammalian expression of ATP2A2

   Depositor

   Jonathan Lytton, Gary Shull

   Article

   Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

   Insert

   ATP2A2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   adenovirus major late promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSG5-FLAG-CaMKKbeta rat RP deletion

   Plasmid

   #32452

   Depositor

   Anthony Means

   Article

   Anderson et al Cell Metab. 2008 May;7(5):377-88.

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deleted RP domain (amino acids 203-225 missing); …

   Promoter

   SV40

   Available since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

10. HT-Pit1

    Plasmid

    #24060

    Depositor

    Frederick Stanley

    Insert

    PIT1 (Pou1f1 Rat)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

11. CALNL-DCX-eGFP

    Plasmid

    #28310

    Depositor

    Joseph Loturco

    Article

    Manent et al Nat Med. 2009 Jan . 15(1):84-90.

    Insert

    Doublecortin (Dcx Rat)
    

    Use

    Cre/Lox

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2011

    Availability

    Academic Institutions and Nonprofits only

12. Cx26-sfGFP

    Plasmid

    #69026

    Purpose

    Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx26 (Gjb2 Rat)
    

    Use

    Tags

    non-monomerized superfolder GFP (V206)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA GAL4 CREB 1-341 M1

    Plasmid

    #22973

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

    Insert

    CREB (Creb1 Rat, GAL4-fusion)
    

    Use

    Tags

    GAL4 DNA-binding domain

    Expression

    Mammalian

    Mutation

    Changed Serine 133 to Alanine of CREB

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHT-497

    Plasmid

    #87756

    Purpose

    Bacterial expression vector for producing 6x histidine tagged rat DYRK1A kinase domain (residues 1-497)

    Depositor

    Yu-Wen Hwang

    Insert

    Gene coding for rat DYRK1A kinase domain (residues 1-497)
    

    Use

    Tags

    6X histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA polymerase promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEN_TT S6K (E389-deltaCT)

    Plasmid

    #58507

    Purpose

    Gateway entry vector for an inducible HA-tagged rat S6K1-E389-deltaCT

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Rps6kb1 (Rps6kb1 Rat)
    

    Use

    Entry vector

    Tags

    HA

    Expression

    Mutation

    T389E, stop codon at amino acid 402

    Promoter

    Available since

    Aug. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pFSW Syt1-C2B IRES GFP

    Plasmid

    #133817

    Purpose

    Encodes the C2B domain of synaptotagmin 1 for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. alpha1Ddelta-G12-FRB

    Plasmid

    #87454

    Purpose

    rapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCs

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    a1D short variants (Cacna1d Rat)
    

    Use

    Tags

    FRB

    Expression

    Mammalian

    Mutation

    delete KYPAKNTTIALQMLERML

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRC/RSV-rat Agt

    Plasmid

    #122104

    Purpose

    Expression of angiotensinogen

    Depositor

    John Chan

    Article

    Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

    Insert

    angiotensinogen (Agt Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV promoter

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUGW-shRIIα-RIIαWT-IRES-EGFP

    Plasmid

    #183456

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. prNFL-P8R-EGFP

    Plasmid

    #132598

    Purpose

    Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    Nefl (Nefl Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    23C>G; Pro8Arg

    Promoter

    CMV

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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