We narrowed to 12,292 results for: BASE

1. pEc-crRNA1

   Plasmid

   #170088

   Purpose

   encodes E. coli type I-E single spacer array

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E single spacer array
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   J23119

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMB-TLEr

   Plasmid

   #61188

   Purpose

   Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   MtRAB7A2
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   MtBCP1

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVP16 AD

   Plasmid

   #165822

   Purpose

   VP16 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   VP16 AD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBN281

   Plasmid

   #86726

   Purpose

   Tissue-specific FLP expression plasmid for C. elegans

   Depositor

   Peter Askjaer

   Article

   Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

   Insert

   Plin-31::FLP D5
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBN258

   Plasmid

   #86721

   Purpose

   Tissue-specific FLP expression plasmid for C. elegans

   Depositor

   Peter Askjaer

   Article

   Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

   Insert

   Punc-119::FLP D5
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJ291_WT_low

   Plasmid

   #128855

   Purpose

   Low copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUPD2

   Plasmid

   #165856

   Purpose

   Universal Part domesticator vector v2 used for integration (domestication) of new DNA elements in the GB2.0 cloning system uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAdh 3'UTR Short

   Plasmid

   #165800

   Purpose

   Drosophila HSP70B 3'UTR sequence

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Hsp70 3'UTR
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDmHsp70b

   Plasmid

   #165785

   Purpose

   Drosophila HSP70B promoter fragment, shows basal expression that can be further enhanced via heatshock

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Hsp70b
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p3xP3-Hsp70b

    Plasmid

    #165773

    Purpose

    HSP70B Promoter fused to synthetic 3xP3 eye driver

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    3x3-Hsp70b
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 5xGMR

    Plasmid

    #165775

    Purpose

    5xGMR syntethic eye driver enhancer, requires minimal promoter element

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    5xGMR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMiniwhiteA1

    Plasmid

    #165872

    Purpose

    Basic transgenesis Mini-white physical eye marker cassette in Alpha1, lacks attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Eye Marker Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FancF_N_FS_v2_Donor

    Plasmid

    #79891

    Purpose

    N-terminal tagging of human FANCF with 3xFLAG_Twin_Strep

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFLAG_Twin_Strep

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pG1-NMGR526

    Plasmid

    #1122

    Depositor

    Susan Lindquist

    Article

    Li et al Science 2000 Jan 28;287(5453):661-4.

    Insert

    sup35 NM domain fused with GR (SUP35, Nr3c1 Rat, Budding Yeast)
    

    Use

    Tags

    GR526

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pHSP70B-CP6-hph

    Plasmid

    #165879

    Purpose

    Basic Hygromycin resistance cassette in Alpha1, lacks physical marker or attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Basic Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p183_LTJ_sgRNACD90.2_A

    Plasmid

    #82671

    Purpose

    sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD90.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJT303_SNR52_sgRNA_Can1-3

    Plasmid

    #145066

    Purpose

    Expresses sgRNA targeting Can1-3 site

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    Can1-3 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p663-UBC-V5-BRSK2_IDG-K

    Plasmid

    #135273

    Purpose

    Gateway destination clone of BRSK2 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-BRSK2 (BRSK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcpmAmetrine

    Plasmid

    #42170

    Depositor

    Robert Campbell

    Article

    Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

    Insert

    cpmAmetrine173
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

20. p663-UBC-miniTurbo-V5-GSK3B_IDG-K

    Plasmid

    #158165

    Purpose

    Gateway destination clone of GSK3B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-GSK3B (GSK3B Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. tdEos-FAK-5

    Plasmid

    #57612

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    FAK
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pJ211_rha_WT_low

    Plasmid

    #128851

    Purpose

    Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJ211_rha_WT_med

    Plasmid

    #128852

    Purpose

    Medium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFA6a-BIO-hphMX4

    Plasmid

    #26880

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    BIO-tag
    

    Use

    Pcr-tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAVPR2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101112

    Purpose

    Encodes dCas9(N) fused to AVPR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    AVPR2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNR186

    Plasmid

    #79552

    Purpose

    Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Insert

    GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMH184

    Plasmid

    #122857

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBN263

    Plasmid

    #86729

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Pmyo-2::FLP D5
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p663-UBC-miniTurbo-V5-ICK_IDG-K

    Plasmid

    #158187

    Purpose

    Gateway destination clone of ICK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-ICK (CILK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

    Plasmid

    #101109

    Purpose

    Encodes dCas9(N)-DHFR fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)-DHFR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFA6a-BIO-TRP1

    Plasmid

    #26881

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    BIO-tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSARSF53-259

    Plasmid

    #61757

    Purpose

    NpuDnaB(delta283)dC39 intein

    Depositor

    Hideo Iwai

    Article

    Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

    Insert

    NpuDnaB(delta283)dC39 intein
    

    Use

    Tags

    H6-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBN237

    Plasmid

    #86719

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Pnhr-82::FLP D5
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGL3_ctrl_CP-candidate_luc+

    Plasmid

    #86392

    Purpose

    Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates basal activity (no enhancer in vector) is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMB-PLAr

    Plasmid

    #61201

    Purpose

    Fluorescent reporter for plastids MtRubisco1sp-mCherry expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtRubisco1sp-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101116

    Purpose

    Encodes dCas9(N) fused to LPAR1

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    LPAR1-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTHSSd_39

    Plasmid

    #59962

    Purpose

    Low core fragment expression (low resource allocator)

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    core fragment
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PJ23105

    Available since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSALECT-Ham1

    Plasmid

    #59706

    Purpose

    pSALECT with Ham1 insert

    Depositor

    Christopher Anderson

    Article

    Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

    Insert

    Ham1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBALU4

    Plasmid

    #69319

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of mChO (mCherry variant)

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    mChOint-FgF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pFA6a-RGS18HIS-TRP1

    Plasmid

    #26884

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    18xHis tag
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge