We narrowed to 18,759 results for: nls

Showing: 2841 - 2880 of 18759 results

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pCSD_2XNLS-SpCas9WT-NLS-3XHA-2XNLS-NmdCas9-NLS

Plasmid

#107327

Purpose

Expresses SpCas9 fused to dNmCas9 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to nuclease dead NmCas9 (D16A, D5…

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13-IDR-YFP-NLS

Plasmid

#145277

Purpose

Mammalian Expression of Nuclear HOXD13 IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Publication

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HOXD13-IDR (HOXD13 Human)

Expression

Mammalian

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-NLSlacZ-stop-L2

Plasmid

#186358

Purpose

Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ Synthetic, E. coli)

Use

Expression of nuclear lacz reporter

Tags

Nuclear localization signal

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS

Plasmid

#145269

Purpose

Mammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS

Depositor

Denes Hnisz

Publication

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TBP(38Q)-IDR (TBP Human)

Expression

Mammalian

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA

Plasmid

#108846

Purpose

Insert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminus

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

nMag,NLS

Expression

Mammalian

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA

Plasmid

#108848

Purpose

Insert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminus

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

pMag,NLS

Expression

Mammalian

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

Plasmid

#167832

Purpose

Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

Depositor

Hugo Snippert

Publication

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

HRAS(N17) (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S17N

Promoter

Ubc, TRE

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Plasmid

#159798

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Plasmid

#159799

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAH36 (Pmyo-2::cGAL-C::let-858 3'UTR)

Plasmid

#107135

Purpose

cGAL-C driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Publication

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Expression

Worm

Promoter

C. elegans myo-2 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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NLS-mAzGreen-Cyclin D1 (HP)

Plasmid

#215072

Purpose

Expresses NLS-mAzGreen-Cyclin D1 (HP) in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Publication

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

NLS-mAzGreen

Expression

Mammalian

Mutation

Cyclin D1(HP) denotes mutations M56A, V60A, and W…

Promoter

LTR

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3omega2_[10x 2EBS-S10:35Smp:3x NLS:3x YPet:35Sterm]-[tNOS:BASTA:pNOS]

Plasmid

#254065

Purpose

3x YPet expression driven by 10x 2EBS-S10

Depositor

Jose Alonso, Anna Stepanova

Publication

Fernandez-Moreno et al Plant Biotechnol J. 2024 Jul;22(7):1942-

Insert

10x 2EBS-S10:35Smp:3x NLS:3x YPet:35Sterm

Use

Synthetic Biology

Expression

Plant

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-C2-P2A-mCherry-NLS

Plasmid

#251137

Purpose

Gachapin-C with a transfection marker mCherry-NLS

Depositor

Takeharu Nagai

Publication

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-C2

Tags

P2A-mCherry-NLS

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-NLS-Mut

Plasmid

#235206

Purpose

Expresses GRP78 with mutated NLS in mammalian cells

Depositor

Amy Shiu Lee

Publication

Liu et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Mutate the NLS sequence

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Publication

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCR1316

Plasmid

#111126

Purpose

Expresses FANCA.

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA (FANCA Human)

Use

Lentiviral

Tags

HA

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

Plasmid

#114090

Purpose

CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human codon optimized MbCas12a

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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L0-I11 PhiC31-NLS GG1-GG4

Plasmid

#195894

Purpose

Level 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkers

Depositor

Jennifer Nemhauser

Publication

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I12 PhiC31-NLS GG2-GG4

Plasmid

#195895

Purpose

Level 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkers

Depositor

Jennifer Nemhauser

Publication

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9 (M14)

Plasmid

#239482

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Publication

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-P2A-BFP

Use

CRISPR

Expression

Plant

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJT131_GalL_cCDA1-NGBE3

Plasmid

#145095

Purpose

Expressing base editor cCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Publication

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-NGBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT130_GalL_nCDA1-NGBE3

Plasmid

#145094

Purpose

Expressing base editor nCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Publication

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-NGBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT79_GalL_nCDA1Δ192-BE3

Plasmid

#145045

Purpose

Expresses nCDA1Δ192-BE3 in yeast cells

Depositor

Ralph Bock

Publication

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ192-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-192AA); spCas9(D10A)

Promoter

GalL

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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P31: FenixS-FUSN-VP16-NLS-IRES-E-Ash1

Plasmid

#250554

Purpose

Mammalian expression of FenixS-FUSN-VP16-NLS and E-Ash1, optogenetic system for far red-light control transcriptional activation

Depositor

Andrew Woolley

Publication

Le et al bioRxiv 2025.11.29.691301

Inserts

FenixS
Ash1

Tags

E protein and FUSN-VP16-NLS

Expression

Mammalian

Promoter

SV40

Available since

Aug. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pSLIK mCh-V5-NES-EGFP-ERBB2 NLS zeo

Plasmid

#192338

Purpose

Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-ERBB2 NLS

Depositor

Kevin Janes

Publication

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-ERBB2 NLS (ERBB2 Human)

Use

Lentiviral

Tags

3xFLAG

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-PGKneo-EF1a-NLS-MARIO-SV40 pA

Plasmid

#247646

Purpose

mammalian expression of NLS-MARIO, a genetically-encoded fluorescent Mg2+ sensor

Depositor

Takeharu Nagai

Publication

Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

Insert

MARIO

Tags

NLS

Expression

Mammalian

Available since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52

Plasmid

#98678

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Publication

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-C1_N-NLS_D652A POLH

Plasmid

#221871

Purpose

Expresses D652A POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Publication

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

D652A

Promoter

CMV

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_K682A_K686A_K694A_K709A POLH

Plasmid

#221872

Purpose

Expresses K682A_K686A_K694A_K709A POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Publication

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

K682A, K686A, K694A, K709A

Promoter

CMV

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_L704A_F707A_F708A POLH

Plasmid

#221873

Purpose

Expresses PIP box-mutated POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Publication

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

L704A_F707A_F708A

Promoter

CMV

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC8-NLS

Plasmid

#207395

Purpose

Expresses AcrIC8 with NLS in mammalian cells

Depositor

Yan Zhang

Publication

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC8-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIE2-NLS

Plasmid

#207396

Purpose

Expresses AcrIE2 with NLS in mammalian cells

Depositor

Yan Zhang

Publication

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIE2-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC9-NLS

Plasmid

#207397

Purpose

Expresses AcrIC9 with NLS in mammalian cells

Depositor

Yan Zhang

Publication

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC9-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1alpha

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-6FLAG (no NLS)

Plasmid

#193322

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

Depositor

Rob Mitra

Publication

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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nPPARg429-gs10-NLS-VPR

Plasmid

#188266

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Publication

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nPPARg429 (PPARG Human)

Expression

Mammalian

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pME-QUASM1:GFPNLS-SV40pA

Plasmid

#155116

Purpose

Middle entry vector containing QUASM1 element upstream of GFP-NLS.

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Gateway middle entry vector

Promoter

QUASM1-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-CHES1L-dC-NLS

Plasmid

#51840

Purpose

Flag-CHES1L mutant lacking the C-terminal, with remaining NLS

Depositor

Gerardo Ferbeyre

Publication

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 1-205

Available since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
T7-5'UTR-IS621-NLS-3'UTR-polyA (CJT503)

Plasmid

#248221

Purpose

IS621-bpNLS IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-IS621-NLS-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3omega2_[EBSn:35Smp:3x SV40-NLS:3x YPet:35Sterm]-[pNOS:KANAMYCIN:tNOS]

Plasmid

#254071

Purpose

3x YPet expression driven by EBSn

Depositor

Jose Alonso, Anna Stepanova

Publication

Fernandez-Moreno et al Plant Biotechnol J. 2026 Feb;24(2):698-7

Insert

EBSn:35Smp:3x SV40-NLS:3x YPet:35Sterm

Use

Synthetic Biology

Expression

Plant

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Plasmid

#217970

Purpose

Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

Depositor

Robert Piper

Publication

Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

Insert

2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Expression

Mammalian

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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We narrowed to 18,759 results for: nls