We narrowed to 4,483 results for: Gaa

Showing: 1421 - 1440 of 4483 results

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pCCL-PGK-SPdCas9-BFP-DNMT1

Plasmid

#66818

Purpose

dCas9 fused to BFP and the human DNMT1 catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dCas9-BFP-DNMT1, catalytic domain (DNMT1 )

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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TRIPZ-CTID195-GSQ-UBnc-PURO

Plasmid

#208037

Purpose

Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

Ubnc (UBC Human)

Use

Lentiviral

Tags

Myc, C-terminal Split-TurboID

Mutation

L73P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET30(a)-AtNUDX23

Plasmid

#175021

Purpose

Purification of AtNUDX23 enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

AtNUDX23 (NUDX23 Mustard Weed)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC763 - pX458 with rat Rosa26 gRNA A

Plasmid

#113161

Purpose

A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat Rosa26

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-FLTID-GSQ-PMLivaWT-P2A-BLAST

Plasmid

#208040

Purpose

Enables constitutive expression of N-terminal TurboID-fused PML (isoform IVa; WT); selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

PML (isoform IVa) (PML Human)

Use

Lentiviral

Tags

FLAG, TurboID

Promoter

EFS

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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TRIPZ-bioGEF-SUMO1nc-PURO

Plasmid

#208042

Purpose

Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

SUMO1nc (SUMO1 Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Mutation

Q94P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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sgTP53(B)

Plasmid

#85560

Purpose

Expresses sgRNA targeting human TP53 around codon F109

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

tumor protein p53 (TP53 Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
MAGI1 gRNA (BRDN0001487062)

Plasmid

#78068

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAGI1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAGI1 (MAGI1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W

Plasmid

#163176

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-HEK3-CTTins-px330-scaffold

Plasmid

#180017

Purpose

Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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shERK1a-mlpx-puro

Plasmid

#65228

Purpose

encodes a shRNA against ERK1

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Expression

Mammalian

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3a

Plasmid

#180432

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-R536C-WLS-HA-IRES-GFP

Plasmid

#178065

Purpose

Expression of WLS-HA protein (R536C mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

R536C

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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B52 + FANCM sgSTOP

Plasmid

#100710

Purpose

B52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting FANCM (cloned using BbsI) (FANCM Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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CDK11B gRNA (BRDN0001146011)

Plasmid

#77065

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11B (CDK11B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pNES-SET-EMD-EGFP (C-NES-SET-EMD)

Plasmid

#217770

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

SET (SET Human)

Tags

EGFP

Expression

Mammalian

Mutation

EMD domain (aa 70-226) plus nuclear export sequen…

Promoter

CMV

Available Since

June 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMXS-IRES-BLAST flag-CAD 3R-3E

Plasmid

#188148

Purpose

Expresses C-terminal flag-tagged human CAD 3R-3E in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W

Plasmid

#163174

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Y478C-WLS-HA-IRES-GFP

Plasmid

#178063

Purpose

Expression of WLS-HA protein (Y478C mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

Y478C

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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