-
Plasmid#131352PurposeExpresses MyD88 with Rip3 fused to its C-terminusDepositorInsertMyD88-Rip3 (Myd88 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cabp5 (445bp crit)-GFP-IRES-AP
Plasmid#18813DepositorInsertCabp5 (445bp crit)-GFP-IRES-AP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-395 HA miRFP670[1] IRES miRFP670[2]
Plasmid#130143PurposePPI (BiFC/TriFC) assay vectorDepositorInsertmiRFP670
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX-MAPKAP1.1-FHH-IRES-Puro
Plasmid#120705PurposeExpresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus productionDepositorInsertMAPKAP1 Isoform 1
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(WT)-IRES-Neo
Plasmid#128663Purposemammalian expression of Flag-Polß(WT) protein with neomycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationWild typePromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES
Plasmid#85750PurposeExpresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2DepositorInsertsType II-C anti-CRISPR AcrIIC3Nme
mTagBFP2
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-MyD88-CpLxIS
Plasmid#131348PurposeExpresses MyD88 with 2 copies of pLxIS motif from STING fused to its C-terminusDepositorInsertMyD88-CpLxIS (Myd88 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/CGA-mEGFP-IRES-mCherry
Plasmid#49233PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast POLE1 C-ISCmut
Plasmid#160804PurposeExpress C-terminal ISC mutant POLE1DepositorInsertPOLE1 C-ISCmut (POLE Human)
UseRetroviralTags3xFLAGExpressionMutationshPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…PromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p7270 MSCV-N-HA-IRES-PURO Empty
Plasmid#163302PurposeEmpty vector controlDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49226PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO deleted ITR
Plasmid#67906PurposePiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeatDepositorInsertNeoR (neoR Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-Tail-8A8G
Plasmid#157981PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (Tail-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLK88 - Edit-directing plasmid base
Plasmid#98814PurposeEdit-directing plasmid baseDepositorInsertCAN1.y gRNA
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/CGA-mEGFP-IRES-mCherry
Plasmid#49224PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Fbxo7(ΔF-box)-IRES-GFP
Plasmid#171787Purposeretrovirus productionDepositorInsertFbxo7 isoform 1 (FBXO7 Human)
UseRetroviralTagsExpressionMutationdeletion of aa335-367PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEJS481-pCDest2-AcrE2-mTagBFP2-IRES
Plasmid#85749PurposeExpresses anti-CRISPR protein from Type I-E and mTagBFP2DepositorInsertsType I-E anti-CRISPR
mTagBFP2
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj10a-IRES-EGFP
Plasmid#164947PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFPDepositorInsertkcnj10a (kcnj10a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRPy CAGGS-Mbd3-IRES-PURO
Plasmid#52376PurposeMammalian expression vector for constitutive expression of Mbd3DepositorInsertMbd3 (Mbd3 Mouse)
UseTags2xFlag (DYKDDK)ExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-tSapphire
Plasmid#176312Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-SapphireDepositorInsertstetR
T-sapphire
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only