We narrowed to 12,366 results for: NUC

1. MLKS2delKASHec

   Plasmid

   #130858

   Purpose

   Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

   Insert

   MLKS2
   

   Use

   Gateway entry vector

   Tags

   eGFP-FLAG-HA

   Expression

   Mutation

   eGFP-FLAG-HA

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pET15b I-SceI (Ctermhis) K223I

   Plasmid

   #195615

   Purpose

   Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C2694G, G2345C, G2351C, A3365T, A3366T

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET15b I-SceI (Ctermhis) K122I

   Plasmid

   #195614

   Purpose

   Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C2458G, A2826T, A2827T

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET PI-SceI C-His K301A

   Plasmid

   #195611

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

   Depositor

   Frederick Gimble

   Article

   He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

   Insert

   PI-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A822G, A823C, A824T

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET PI-SceI C-His

   Plasmid

   #195610

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag

   Depositor

   Frederick Gimble

   Article

   He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

   Insert

   PI-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…

   Promoter

   T7

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAcrVIB1-cloDF-amp

   Plasmid

   #184843

   Purpose

   Expression of anti-CRISPR protein AcrVIB1 in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   anti-CRISPR protein inhibiting Cas13b nucleases
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAcrVIB1-cloDF-kan

   Plasmid

   #184836

   Purpose

   Expression of anti-CRISPR protein AcrVIB1 in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   anti-CRISPR protein inhibiting Cas13b nucleases
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR-ZmIAA29 (yeast codon optimized)

   Plasmid

   #188441

   Purpose

   Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

   Depositor

   Britney Moss

   Article

   Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

   Insert

   Auxin Signaling Repressor IAA29 (yeast codon optimized)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK528

   Plasmid

   #155388

   Purpose

   dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLS

    Plasmid

    #121827

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-Cas9 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KHLH31 pEGFP-N2 (1089)

    Plasmid

    #62032

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    KHLH31
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. NET33 pEGFP-N2 (594)

    Plasmid

    #61987

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET33 (SCARA5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. RFN_EGFP_84

    Plasmid

    #60076

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. RFN_EGFP_83

    Plasmid

    #60075

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

15. RFN_EGFP_82

    Plasmid

    #60074

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

16. RFN_EGFP_80

    Plasmid

    #60072

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pJK520

    Plasmid

    #155386

    Purpose

    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid

    #134470

    Purpose

    Human expression vector for SaCas9-HF variant.

    Depositor

    Zongli Zheng

    Article

    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

    Insert

    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    

    Use

    CRISPR

    Tags

    SV40NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    R245A, N413A, N419A, R654A

    Promoter

    CAG

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Emerin pEGFP-N2 (588)

    Plasmid

    #61985

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Emerin (EMD Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-7 asyn WT

    Plasmid

    #36046

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge