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Plasmid#100728PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform, deletion of clathrin box motifPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRC2_p53DD
Plasmid#136520PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertp53DD (Trp53 Mouse)
UseEntry vector for gateway cloningTags3x FLAGExpressionMutationThis construct was created by PCR from the pBabe …PromoterAvailable sinceOct. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP Y215A
Plasmid#100727PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform Y215A mutation (Y815A in full length)PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
SSH-pSUMO-β′-COPI-WD40-Lys17Ala
Plasmid#208171PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Lys17Ala mutant (residues 1-301)DepositorInsertN-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Lys17Ala substitution
UseTagsHis-tag, SUMO-tag, and Strep-tagExpressionBacterialMutationLys17Ala substitutionPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAcBac3-EcLeu TAG-EGFP**
Plasmid#228785PurposeEcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppressionDepositorInsertsEcLeuRS
EcLtR
EGFP**
UseBaculoviralTagsExpressionMammalianMutationTAG suppressorPromoterAvailable sinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA GPR153
Plasmid#226812PurposeTet-inducible lentiviral vector for 2xHA GPR153 expressionDepositorInsertGPR153 (GPR153 Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_997253.2PromoterAvailable sinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-BD-SARS-CoV-2-S-tail-WT
Plasmid#218455PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA LTB4R
Plasmid#226810PurposeTet-inducible lentiviral vector for 2xHA LTB4R expressionDepositorInsertLTB4R (LTB4R Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_858043.1PromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA GPR153
Plasmid#226808PurposeGateway entry vector for 2xHA GPR153DepositorInsertGPR153 (GPR153 Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_997253.2PromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA LTB4R
Plasmid#226806PurposeGateway entry vector for 2xHA LTB4RDepositorInsertLTB4R (LTB4R Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_858043.1PromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA GPR137
Plasmid#226813PurposeTet-inducible lentiviral vector for 2xHA GPR137 expressionDepositorInsertGPR137 (GPR137 Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_001164351PromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAcBac3-EcTrp-TGA-EGFP**
Plasmid#228786PurposeEcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppressionDepositorInsertsEcTrpRS
EcWtR
EGFP**
UseBaculoviralTagsExpressionMammalianMutationTGA suppressorPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-TRPML1(PHD)
Plasmid#221752PurposeStable expression of GFP-TRPML1(PHD) in mammalian cellsDepositorInsertTRPML1 (MCOLN1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem TRPML1(PHD) (amino acids 1–69)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala
Plasmid#208172PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
UseTagsStrep-tagExpressionMammalianMutationArg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala
Plasmid#208173PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
UseTagsStrep-tagExpressionMammalianMutationLeu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala
Plasmid#208174PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
UseTagsStrep-tagExpressionMammalianMutationLys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
JO-pH720-SARS-CoV-2-S-tail-WT
Plasmid#218453PurposeBacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike with an N-terminal eXact tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag (S SARS-CoV-2)
UseTagseXactExpressionBacterialMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala
Plasmid#218463PurposeMammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions)DepositorInsertSARS-CoV-2 spike protein (1273 residues) carrying Lys1269Ala and His1271Ala substitutions (S )
UseTagsExpressionMammalianMutationLys1269Ala and His1271Ala substitutionsPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-Aurora B KD
Plasmid#184043PurposeExpresses H2B-GFP fused to Aurora B kinase deadDepositorInsertAURKB (AURKB Human)
UseTagsEGFP and H2BExpressionMammalianMutationChanged Lysine 65 to ArgininePromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Repo-Man-1-890
Plasmid#184272PurposeExpresses C-terminally truncated GFP-RepoManDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTagsGFPExpressionMammalianMutationtruncated Repo-Man after aminoacid 893PromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only