Addgene

We narrowed to 12,997 results for: SHI

Showing: 3601 - 3650 of 12997 results

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pMRX-IB-HaloTag7-mGFP-KDEL

Plasmid

#184904

Purpose

Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

KDEL

Use

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PTEN

Plasmid

#28298

Depositor

Maria-Magdalena Georgescu

Article

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-puro-LacZ

Plasmid

#39477

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

LacZ

Use

Lentiviral

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pSM.wrmScarlet1-10::SL2::GFP1-10

Plasmid

#234318

Purpose

For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans

Depositor

Kota Mizumoto

Article

Kurashina et al Genetics. 2024 Dec 22:iyae214. doi: 10.1

Insert

wrmScarlet1-10::SL2::GFP1-10

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRK-Myc-Smurf1

Plasmid

#13676

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 (SMURF1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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mVenus-pBAD

Plasmid

#54845

Purpose

Localization: Protein Expression Vector , Excitation: 515, Emission: 528

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Use

Tags

6xHis and mVenus

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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FOP-GFP.mC

Plasmid

#35492

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xFOP-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mutated 7xTCF/LEF

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pSAM

Plasmid

#45174

Depositor

Jumi Shin

Article

Sathiamoorthy et al PLoS One. 2012;7(10):e47259. doi: 10.137

Type

Empty backbone

Use

Tags

6xHis and 6xHis/Thrombin/T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+hSpCas9

Plasmid

#51815

Purpose

Encode hSpCas9 for in vitro transcription with Sp6

Depositor

Masato Kinoshita

Article

Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

Insert

humanized S.pyogenes Cas9

Use

CRISPR; Medaka, zebrafish

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pRS405-LEU2-TEF-KpolDcr1v2

Plasmid

#85193

Purpose

Integrating plasmid, K. polysporus Dcr1 under TEF promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Dcr1

Use

Integrating, shuttle vector

Tags

Expression

Yeast

Mutation

Promoter

TEF

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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FOP-GFP

Plasmid

#35490

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xFOP-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mutated 7xTCF/LEF

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
poLuc1

Plasmid

#154128

Purpose

Expresses orthogonal firefly luciferase gene in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

orthogonal firefly luciferase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINVL

Plasmid

#26040

Depositor

Ray Owens

Article

Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

Insert

u-LacZ

Use

Tags

u-phosphatase secretion leader

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pOPINVH

Plasmid

#26041

Depositor

Ray Owens

Article

Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

Insert

u-LacZ-KHHHHHH

Use

Tags

KHHHHHH and u-phosphatase secretion leader

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pBEST-OR2-OR1-Pr-UTR1-deGFP-T500

Plasmid

#40019

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-LC3

Plasmid

#184900

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-2a-tdTomato

Plasmid

#67707

Purpose

3' entry 2A-tdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

2A-tdTomato

Use

Tags

Expression

Mutation

G172S (please see depositor's comments below)

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMMK803

Plasmid

#207138

Purpose

Assembled Tn10 with CP25 promoter driving gfp

Depositor

Nicholas Shikuma

Article

Alker et al ISME Commun. 2023 Sep 19;3(1):98. doi: 1

Insert

Tn10-CP25-GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

CP25

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNC Wnt-5aHA

Plasmid

#18032

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5a (Wnt5a Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-7aHA

Plasmid

#18036

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

WNT-7A (Wnt7a Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pProm1_BCD1-GFP

Plasmid

#80649

Purpose

Promoter1 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

NONE

Promoter

constitutive promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p175 Grg-1s HA

Plasmid

#11064

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-S (Tle1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-1HA

Plasmid

#18027

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-1 (Wnt1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
p3E-2a-6MTnlstdTomato

Plasmid

#67699

Purpose

3' entry clone with 6xmyc nuclear localized tdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

tdTomato

Use

Tags

2A-6xMyc-NLS

Expression

Mutation

Promoter

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Wnt-1HA

Plasmid

#17367

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-1HA (Wnt1 Mouse)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pBABE-FLAG-HA-hG9a

Plasmid

#33022

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-7bHA

Plasmid

#18037

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-7b (Wnt7b Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-4HA

Plasmid

#18031

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-4HA (Wnt4 Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pBS Wnt-4HA

Plasmid

#17375

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-4HA (Wnt4 Mouse)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pBS Wnt-5aHA

Plasmid

#17376

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5aHA (Wnt5a Mouse)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-3HA

Plasmid

#18029

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3 (WNT3 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-6HA

Plasmid

#18035

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-6 (Wnt6 Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pBS Wnt-5bHA

Plasmid

#17377

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5bHA (Wnt5b Mouse)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pABCb10aa1-70-GFP

Plasmid

#19038

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

amino acids 1 - 70 of the targeting sequence of ABCb1

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pRBS wt

Plasmid

#154126

Purpose

Expresses wt rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

wt bacterial rRNAs operon under control of pL promotor

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-FLAG-hG9a

Plasmid

#33023

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pBS Wnt-7aHA

Plasmid

#17379

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-7aHA (Wnt7a Mouse)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pISH-Cbr2

Plasmid

#74339

Purpose

ISH probe for Cbr2

Depositor

Peter Mombaerts

Article

Ishii et al J Neurocytol. 2004 Dec;33(6):657-69. Epu

Insert

carbonyl reductase 2 (Cbr2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only
pBS Wnt-2HA

Plasmid

#17368

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-2HA (WNT2 Human)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pRET.IL.IRES-EGFP (No HSV-TK)

Plasmid

#1836

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-2

Plasmid

#17992

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-2 (Wnt2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-2HA

Plasmid

#18028

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-2 (WNT2 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pProm11_BCD1-GFP

Plasmid

#80656

Purpose

Promoter11 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LHP585 - Ub-CYC1

Plasmid

#32168

Depositor

Linda Hicke

Article

Shih et al EMBO J. 2003 Mar 17;22(6):1273-81.

Insert

uba1 yeast (UBA1, UBA2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-5bHA

Plasmid

#18034

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5b (Wnt5b Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pRET.IS.IRES-EGFP N2

Plasmid

#1834

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pTLR-donor-tetO

Plasmid

#119000

Purpose

Repair template for the TLR reporter with 7 tet operator elements

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pProm9_BCD1-GFP

Plasmid

#80655

Purpose

Promoter9 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Wnt-6HA

Plasmid

#17378

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-6HA (Wnt6 Mouse)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
p177 Grg-1s EGFP

Plasmid

#11066

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-S (Tle1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3601 - 3650 of 12997 results

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