We narrowed to 16,108 results for: nans
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Plasmid#134342PurposeNanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi2-91-NLuc
Plasmid#207149PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαq-124-NLuc
Plasmid#207153PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα13-125-NLuc
Plasmid#207155PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP261
Plasmid#104166PurposeBacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labelingDepositorInsertAnti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
Tags14x Histidine tag and NEDD8 from Brachypodium dis…ExpressionBacterialMutation1x Cysteine located at bps 493-495 in full sequen…Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-Tom20-Halo7Tag
Plasmid#111135PurposeTom20 with selflabeling tag, e.g. for fluorescence microscopyDepositorInsertReceptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
TagsHalo7 TagExpressionMammalianPromoterCMVAvailable SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-Halo7Tag-hFis
Plasmid#111136PurposehFis with selflabeling tag, e.g. for fluorescence microscopyDepositorInserthuman fission factor1 (FIS1 Human)
TagsHalo7-tagExpressionMammalianMutation*PromoterCMVAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW-NbVHH05-mCherry
Plasmid#171572PurposeExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cellsDepositorAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Xph15-GCaMP6f
Plasmid#135538PurposeEncodes a specific PSD-95 binder (Xph15) fused to GCaMP6fDepositorInsertXph15
TagsGCaMP6fExpressionMammalianPromoterCMVAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cav3-YFP
Plasmid#69507PurposeExpression of fluorescently tagged Caveolin-3 in mammalian cellsDepositorAvailable SinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro3-p16Flag
Plasmid#24934DepositorAvailable SinceJuly 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAW-NbVHH05-GFP
Plasmid#171570PurposeExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cellsDepositorAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD P35S (GB0552)
Plasmid#160554Purpose35s promoter for N-terminal fusionsDepositorInsert35s
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro3-p18Flag
Plasmid#24933DepositorAvailable SinceJuly 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-std
Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAd5-Blue
Plasmid#174431PurposepAd5-Blue provides a platform for the rapid construction of recombinant and replication defective Adenovirus. This vector contains unique restriction enzyme sites to allow cloning of a gene.DepositorInsertβ-galactosidase α gene fragment
UseAdenoviralExpressionMammalianPromoterCMVAvailable SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro3-p18
Plasmid#24935DepositorAvailable SinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
(363) pcDNA3.1-Nup62-Ubc-Flag-EPEA
Plasmid#168098PurposeNucleoporin Nup62 with C-terminal Ubc nanobody tag, Flag tag, and EPEA nanobody tagDepositorInsertNup62-Ubc-Flag-EPEA
TagsEPEA-tag, Flag-tag, and Ubc-TagExpressionMammalianMutationP229A, S283T on Nup62 native sequencePromoterT7/cmvAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28b Salmonella NAT_6H C-terminal
Plasmid#223436PurposeSalmonella NAT expression vector with C-terminal His6 tagDepositorInsertSalmonella NAT
TagsHis6Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only