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DHFR-PP7-VP64_T2A_GFP

Plasmid

#86167

Purpose

Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-PP7-VP64_T2A_GFP

Use

CRISPR and Lentiviral

Tags

Destabilized domain (N-terminus version) of E.col…

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

Plasmid

#63169

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-FLAG"

Tags

N-myristoylation

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ909

Plasmid

#44480

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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UASGAL-Pgal

Plasmid

#60338

Purpose

Contains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1-scrCdh20.1 GFP

Plasmid

#209106

Purpose

GFP expressing scramble of shRNA targeting Cdh20

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh20.1

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pFosill-1

Plasmid

#89695

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDBTrp-LexABD-CRY2FL

Plasmid

#78210

Purpose

Use with Addgene 40615 for blue light transcription control in yeast

Depositor

Chandra Tucker

Article

Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

Insert

LexABD-CRY2 (CRY2 Mustard Weed)

Tags

lexA binding domain

Expression

Yeast

Mutation

contains endogenous CRY2 NLS

Promoter

ADH1

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBJ1 neo-hu FceRI gamma

Plasmid

#16540

Depositor

Jean Kinet

Article

Kuster et al J Biol Chem 1990 Apr 15;265(11):6448-52.

Insert

FCER1G (FCER1G Human)

Expression

Mammalian

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101103

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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P[acman]-Alpha2

Plasmid

#165859

Purpose

Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_WTDHFR_WTTS

Plasmid

#91226

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-intron

Plasmid

#177804

Purpose

An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

intron

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Pdgfra-T2A-H2B-eGFP donor

Plasmid

#113120

Purpose

Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Synthetic, Mouse)

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ906

Plasmid

#44472

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, [4-3] Gateway

Use

Gateway destination vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pFosill-3

Plasmid

#89697

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGinB-8X(GGS)-dCas9-FLAG-NLS

Plasmid

#81205

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-TEX264 LIR4A-FLAG

Plasmid

#128259

Purpose

Expresses TEX264 LIR4A in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

TEX264 LIR4A (TEX264 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed FEEL (aa 273-276) to alanines

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMSP2

Plasmid

#26282

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP2

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of dimer of deletion mutant (1-43)…

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
pFosill-2

Plasmid

#89696

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 mFz8CRD-IgG

Plasmid

#16689

Depositor

Xi He

Article

Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

Insert

Frizzled-8 (Fzd8 Mouse)

Tags

IgG

Expression

Mammalian

Mutation

CRD domain of mFz8

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-nucGFP11-3xControlgRNA

Plasmid

#224568

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

GFP11-H2B

Use

CRISPR

Tags

Histone H2B (nuclear localization)

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-V96 ELP

Plasmid

#67853

Purpose

GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

Depositor

Andrew MacKay

Article

Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

Insert

GFP fused to ELP (VPGVG) repeated 96 times

Tags

GFP fused to N-terminal of ELP

Expression

Mammalian

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - CA

Plasmid

#65424

Purpose

RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, Q63L in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
RhoC FLARE.sc mCer, mVenus - DN

Plasmid

#65423

Purpose

RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, T19N in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
EYFP-Clathrin

Plasmid

#20921

Depositor

Xiaowei Zhuang

Article

Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

Insert

Clathrin, light polypeptide (Lca) (Clta Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pFBD-ESCRT 0

Plasmid

#21499

Depositor

James Hurley

Article

Ren et al Structure. 2009 Mar 11. 17(3):406-16.

Insert

pFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Mouse, Human)

Tags

GST and His-MBP

Expression

Insect

Mutation

none

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pFosill-4

Plasmid

#89698

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

Plasmid

#183069

Purpose

Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Use

CRISPR

Tags

His10 and MBP

Expression

Bacterial

Mutation

Wild-type

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR H215R

Plasmid

#13509

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR H215R (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Mutation

H215R

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only
pNAS1B split mCherry N-link/link-C

Plasmid

#61965

Purpose

Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-link
link-C-mCherry

Expression

Bacterial

Promoter

PBAD and PLtetO

Available Since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

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