We narrowed to 31,545 results for: Eng;
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Plasmid#178519PurposeFor making TRE-CB mouse line in Tigre locusDepositorInsertmCardinal-P2A-B19L
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-SpRY
Plasmid#161520PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoterDepositorInsertzSpRYCas9
UseCRISPR; Gateway compatible zsprycas9 entry cloneTags3X FLAG, NLS and NLSExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUT8-Dox
Plasmid#124631PurposeDoxycycline-inducible FUT8 expression in mammalian cellsDepositorInsertsAlpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GACh2.0
Plasmid#121921Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0DepositorInsertGPCR activation based ACh sensor GACh2.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262-ABE8e
Plasmid#161523PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editingDepositorInsertABE8e-zSpCas9(D10A)
UseCRISPR; Gateway compatible abe8e-zspcas9(d10a) en…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA
Plasmid#178518PurposeFor making Rabies virus SAD B19 strain L cell line with hyPBaseDepositorInsertB19L-IRES-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E6
Plasmid#213469PurposeGateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cda-32aa linker-z…TagsNoExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266E
Plasmid#161521PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editingDepositorInsertzSpRYCas9(D10A)-PmCDA1-UGI
UseCRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…Tags3X FLAG, NLSExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262B-ABE8e
Plasmid#161524PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editingDepositorInsertTadA8e-zSpRYCas9(D10A)
UseCRISPR; Gateway compatible tada8e-zsprycas9(d10a)…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shOrai1
Plasmid#89933PurposeKnockdown human Orai1 expressionDepositorInsertOrai1 shRNA (ORAI1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
PITX3-eGFP-PGK-Puro
Plasmid#31942DepositorInsertPITX3 (PITX3 Human)
UseTargeting donorTagseGFPExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A
Plasmid#111455PurposeTransient expression of Flag-tagged human Sox9 with T236A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT236A mutationPromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GACh1.0
Plasmid#106072Purposeexpress the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cellsDepositorInsertGPCR activation based ACh sensor (GACh) 1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shSTIM1
Plasmid#89816PurposeKnockdown human STIM1 expressionDepositorInsertSTIM1 shRNA (STIM1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)
Plasmid#99229PurposeMammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.DepositorInsertpr-mEos2
UseTagsLifeactExpressionMammalianMutationA69T for primed conversion applicationsPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-Smad4
Plasmid#89829PurposeKnockdown human Smad4 expression in human mammalian cellsDepositorInsertSmad4 shRNA (SMAD4 Human, Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T240A
Plasmid#111457PurposeTransient expression of Flag-tagged human Sox9 with T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
UseTagsExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mGSDMD1-251
Plasmid#111565PurposeExpress ELANE-cleaved mouse GSDMD in mammalian cellsDepositorInsertGasdermin D (Gsdmd Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A/T240A
Plasmid#111458PurposeTransient expression of Flag-tagged human Sox9 with T236A/T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT236A and T240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJS699
Plasmid#168779PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombinationDepositorInsertSpike RBD (S betacoronavirus)
UseTagsCmycExpressionMutationN343QPromoterGAL1Available sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
B4GALT1-Dox
Plasmid#124633PurposeDoxycycline-inducible B4GALT1 expression in mammalian cellsDepositorInsertsBeta-(1,4)-Galactosyltransferase 1
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRPyCAG-fmKlf4-DsRed-Ip
Plasmid#26274DepositorInsertKlf4 (Klf4 Mouse)
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m
Plasmid#161522PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editingDepositorInsertTadA(wt)-TadA(7.10)-zSpCas9(D10A)
UseCRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C72A
Plasmid#122477Purposeexpresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …PromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PatoM-LOG241
Plasmid#72847PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. LOG241 backbone.DepositorInsertPatoM
UseTagsExpressionBacterialMutationPromoterPatoMAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmiR-96 cluster promoter
Plasmid#62517PurposemicroRNA 183-96-182 cluster promoter luciferase plasmidDepositorInsertpromoter of microRNA-183-96-182 cluster
UseTagsRenSP (optimized renilla luciferase gene)ExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
IFITM3-N21del-V93-TAG
Plasmid#122480Purposeexpresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsExpressionMammalianMutationChanged Valine 93 to amber codon and deleted amin…PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CNC3G (CASANOVA-C3 with glycine linkers)
Plasmid#137192PurposeExpresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory proteinDepositorInsertCNC3G (CASANOVA-C3 with glycine linkers)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
NG1301 pCI-TPI-4MS2-SMG5(short)-4H
Plasmid#65806PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4 MS2 stem loops, SMG5 3'UTR shortened versi…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C105A
Plasmid#122478Purposeexpresses human IFITM3-C105A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …PromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-V93-TAG
Plasmid#122479Purposeexpresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Valine 93 to amber codonPromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C71A
Plasmid#122476Purposeexpresses human IFITM3-C71A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …PromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-Smad3
Plasmid#89828PurposeKnockdown human Smad3 expression in human mammalian cellsDepositorInsertSMAD3 shRNA (SMAD3 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCSG49-AlleyCat7
Plasmid#42591DepositorInsertmutated C-terminal portion of calmodulin gene (M76-K148)
UseTagsHis and TEVExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Isole…PromoterT7Available sinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
ubbr:GCaMP8m
Plasmid#232485PurposeTo make fish transgenic lines that have calcium sensor expression in the whole body of the fishDepositorInsertjGCaMP8m
UseZebrafish expressionTagsExpressionMutationPromoterubbrAvailable sinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAD100-CHIP-myc-His
Plasmid#236054PurposeMammalian expression of human CHIP with a C-terminal myc-His tagDepositorInsertCHIP (STUB1 Human)
UseTagsmyc-HisExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC TtoS
Plasmid#226608PurposeAll threonines to serineDepositorInsertFUS LC 4QQ->GG (FUS Human)
UseTags6HisExpressionBacterialMutation4 QQ pairs replaced with GG pairsPromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC QtoA
Plasmid#226609PurposeAll glutamines to alanineDepositorInsert6*His-TEV-FUS LC QtoA (FUS Human)
UseTags6HisExpressionBacterialMutationAll glutamine to alaninePromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC 12S->G
Plasmid#226597Purpose12 serines to glycinesDepositorInsertFUS LC 12S->A (FUS Human)
UseTags6HisExpressionBacterialMutation12 serines to alaninePromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only