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pYL156 (TRV RNA2)

Plasmid

#148969

Purpose

Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

Depositor

Savithramma Dinesh-Kumar

Article

Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

Insert

Modified TRV RNA2

Use

T-dna vector

Mutation

see comments section below

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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FUCas9Cherry

Plasmid

#70182

Purpose

Expresses Cas9 with fluorescent Cherry reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

humanised S.pyogenes cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

hUbC

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLNCX2 ER:ras

Plasmid

#67844

Purpose

4-hydroxytamoxifen inducible ER:ras fusion protein

Depositor

Masashi Narita

Article

Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

Insert

ER1a H-RasG12V fusion

Expression

Mammalian

Mutation

G525R renders the HBD largely insensitive to 17ß‐…

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe HA-Runx1-neo

Plasmid

#45815

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

Runx1 (Runx1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
shCTL-mlpx-puro

Plasmid

#65232

Purpose

encodes a non-targeting control shRNA

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

non-coding shRNA

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pYihI-N+C-term K-R

Plasmid

#233093

Purpose

Expression of GST-YihI with N+C-term lysines (K) mutated to arginine (R)

Depositor

Michael Downey

Article

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

GST-YihI with N+C-term lysines (K) mutated to arginine (R)

Tags

GST

Expression

Bacterial

Mutation

GST-YihI with N+C-term lysines (K) mutated to arg…

Available Since

May 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pYihI-All K-R

Plasmid

#233094

Purpose

Expression of GST-YihI with all lysines (K) mutated to arginine (R)

Depositor

Michael Downey

Article

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

GST-YihI with all lysines (K) mutated to arginine (R)

Tags

GST

Expression

Bacterial

Mutation

GST-YihI with all lysines (K) mutated to arginine…

Available Since

May 9, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pYihI-N-term S-A

Plasmid

#233096

Purpose

Expression of GST-YihI with N-term serines (S) mutated to alanine (A)

Depositor

Michael Downey

Article

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

YihI with N-term serines (S) mutated to alanine (A)

Tags

GST

Expression

Bacterial

Mutation

N-term serines (S) mutated to alanine (A)

Available Since

May 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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shCTL-mlpx-neo

Plasmid

#65233

Purpose

encodes a non-targeting control shRNA

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

non-coding shRNA

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pYL192 (TRV1)

Plasmid

#148968

Purpose

Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

Depositor

Savithramma Dinesh-Kumar

Article

Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

Insert

TRV RNA1

Use

Plant vector with trv rna1; this vector used with…

Mutation

P697S mutation in putative replicase. S239G and S…

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP (empty vector)

Plasmid

#172604

Purpose

Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Type

Empty backbone

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSBtet rtTA G72V SE Δsplice GP Luc

Plasmid

#194328

Purpose

Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness

Depositor

Alexander McLellan

Article

Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:

Insert

Luciferase

Use

Luciferase

Expression

Mammalian

Mutation

Tet-responsive promotor related mutations: G72V i…

Promoter

Tight tet-responsive promotor

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pYB-Dual(mNG(yeast-opt))

Plasmid

#216727

Purpose

Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.

Depositor

Emil Jensen

Article

Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:

Insert

mNeonGreen

Expression

Bacterial and Yeast

Promoter

pGAL1(yeast) and pT7-RNAP(bacteria)

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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Alt AlphaPS3

Plasmid

#14070

Depositor

Richard Hynes

Article

Stark et al Development. 1997 Nov . 124(22):4583-94.

Insert

Drosphila Alt Alpha PS3 Integrin (scb Fly)

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pLV-PGK-dt/CBA-EGFP-NLS

Plasmid

#163918

Purpose

Contains bidirectional reporter genes dtomato and nuclear-targeting EGFP.

Depositor

John Engelhardt

Article

Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

Insert

dtomato and EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

Added nuclear localization signal to EGFP

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMS22

Plasmid

#129710

Purpose

Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)

Tags

10-Histidine tag cleavable with PreScission Prote…

Expression

Bacterial

Mutation

RssB E134G:E135S:E136G:E137S

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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Comp AlphaPS3

Plasmid

#14071

Depositor

Richard Hynes

Article

Stark et al Development. 1997 Nov . 124(22):4583-94.

Insert

Drosphila Comp Alpha PS3 Integrin (scb Fly)

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pTRIPZ-TIR1-HA

Plasmid

#172610

Purpose

Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

TIR1

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe DN-FOXO1-HA neo

Plasmid

#45814

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

FOXO1 (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

aa1-255

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-MBP-(CBX1)3-WT

Plasmid

#180212

Purpose

Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1 x3 (CBX1 Human)

Tags

6xhis-tag and MBP

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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