19,619 results

1. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Plasmid

   #179105

   Purpose

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. wtBxb1

   Plasmid

   #222337

   Purpose

   Plasmid for expressing wild type Bxb1 in mammalian cells

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized wild type Bxb1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAIDFA-EF1a-NmScarlet-mAID

   Plasmid

   #140615

   Purpose

   Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1 mutated F74A
   

   Expression

   Mammalian

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti.PGK.blast-Renilla_Luciferase

   Plasmid

   #74444

   Purpose

   Control lentiviral vector expressing Renilla luciferase

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Renilla Luciferase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30ax

   Plasmid

   #85761

   Purpose

   A bacterial expression vector with a His-tag at N and C-terminus.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Type

   Empty backbone

   Tags

   His tag (N-term and C-term)

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Myc-TEAD4

   Plasmid

   #24638

   Depositor

   Kunliang Guan

   Article

   Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

   Insert

   TEAD-4 (TEAD4 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   May 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

   Plasmid

   #20298

   Purpose

   Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   channelrhodopsin-2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   humanized ChR2 gene with histidine 134 changed to…

   Promoter

   EF1alpha

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUW-tetO-hOKMS

   Plasmid

   #51543

   Purpose

   Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

   Depositor

   Tarjei Mikkelsen

   Article

   Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

   Insert

   hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-N-Flag-NLRP3

   Plasmid

   #75127

   Purpose

   Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEGFP-C1

    Plasmid

    #54759

    Purpose

    Localization: C1 Cloning Vector, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pTRIP-CMV-tagRFP-FLAG-cGAS

    Plasmid

    #86676

    Purpose

    Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    tagRFP-FLAG-cGAS
    

    Use

    Lentiviral

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mOrange2-pBAD

    Plasmid

    #54531

    Purpose

    Localization: Protein Expression Vector , Excitation: 549, Emission: 565

    Depositor

    Michael Davidson, Nathan Shaner, Roger Tsien

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Tags

    6xHis and mOrange2

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. VPR-ps-dSpCas9

    Plasmid

    #102855

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    dSpCas9, pdDronpa1.2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS and VP64-p64-Rta transcription activator

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDA_118

    Plasmid

    #133459

    Purpose

    U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRC0569 (parts entry vector)

    Plasmid

    #189966

    Purpose

    Cloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Promoter

    BBa-J23100

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. LV-Cre pLKO.1

    Plasmid

    #25997

    Purpose

    Lentiviral expression of nls-Cre from the CMV promoter

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

    Plasmid

    #83841

    Purpose

    Fluorescent probe for G1/S transition and M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4.10-VEGFprom(-1000 to -1)

    Plasmid

    #66128

    Purpose

    Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp)

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)
    

    Use

    Luciferase

    Promoter

    VEGF -1000 to -1

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRX-INU-FLAG-FAM134B

    Plasmid

    #128260

    Purpose

    Expresses FAM134B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134B (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1.Gαs-LgB113

    Plasmid

    #134362

    Purpose

    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαs-LgB113 (GNAS Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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