We narrowed to 12,157 results for: pcdna

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pcDNA3-Akt-STOPS

Plasmid

#175006

Purpose

Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-STOPS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos WT

Plasmid

#8966

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB2b

Plasmid

#19524

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB2b (DNAJB2 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNBAS_AA

Plasmid

#148236

Purpose

Mammalian Expression of HsNBAS

Depositor

Elisa Izaurralde

Insert

HsNBAS (NBAS Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-600u

Plasmid

#13572

Depositor

Wolf Frommer

Article

Fehr et al J Biol Chem. 2003 May 23. 278(21):19127-

Insert

FLIPglu F16A

Tags

ECFP and EYFP

Expression

Mammalian

Mutation

F16A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA3-hTICAM1-VenusFlag

Plasmid

#197632

Purpose

Human TRIF protein expression in mammalian cells

Depositor

Haian Fu

Insert

TICAM1 (TICAM1 Human)

Tags

Venus-Flag

Expression

Mammalian

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL

Plasmid

#24233

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Hu-Trip6

Plasmid

#27255

Depositor

Thomas Gilmore

Article

Zhao et al Gene Expr. 1999 . 8(4):207-17.

Insert

Trip6 (TRIP6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 (-) hYAP 1-2alpha

Plasmid

#124143

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRIPK1-Flag

Plasmid

#112487

Purpose

Mammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.

Depositor

Junying Yuan

Insert

RIPK1 (RIPK1 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AKAR

Plasmid

#181844

Purpose

Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-AKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-RIIα-sREACh

Plasmid

#168489

Purpose

Mammalian expression of mouse PKA regulatory subunit IIα C-terminally tagged by sREACh.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

PKA regulatory subunit IIα C-terminally tagged by sREACh

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-humanTRAF3 (1098)

Plasmid

#66927

Purpose

Mammalian Expression of TRAF3

Depositor

Michael Karin

Insert

TRAF3 (TRAF3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-PANX1(HCVp)

Plasmid

#87698

Purpose

Express HCV protease-activatible pannexin-1 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PANX(HCVp)

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CRISPR-Puro

Plasmid

#208646

Purpose

pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

Depositor

Quanfu Ma

Type

Empty backbone

Use

CRISPR

Tags

Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

Expression

Mammalian

Promoter

CMV and U6 in tandem

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Barr2 flag

Plasmid

#14685

Depositor

Robert Lefkowitz

Article

Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

Insert

beta-arrestin 2 (Arrb2 Rat)

Tags

flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-OTI-TCRB_p2_TCRA

Plasmid

#131034

Purpose

Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Inserts

TCRB (Tcrb Mouse)
TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4

Plasmid

#14615

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-AKARev

Plasmid

#118478

Purpose

ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Dvl2

Plasmid

#123587

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

dishevelled segment polarity protein 2 (Dvl2 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-p27

Plasmid

#19937

Depositor

Yue Xiong

Article

Zariwala et al Oncogene. 1998 Nov 26. 17(21):2787-98.

Insert

CDKN1B cyclin-dependent kinase inhibitor 1B (CDKN1B Human)

Tags

myc3

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pF152 pcDNA3.1(+)SOD1A4V

Plasmid

#26398

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

A4V

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA1 Flag ATXN1[2Q]

Plasmid

#33235

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Tags

Flag

Expression

Mammalian

Mutation

insert contains 2Q (2 glutamines) and there isno …

Promoter

CMV

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-10His-StayGold

Plasmid

#211362

Purpose

Mammalian expression of the bright and photostable StayGold fluorescent protein. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

StayGold

Tags

10xHis-tag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/CyclinB1-GFP (28)

Plasmid

#39871

Depositor

Jonathon Pines

Article

Jackman et al Mol Biol Cell. 2002 Mar;13(3):1030-45.

Insert

CyclinB1 (CCNB1 Human)

Tags

GFP

Expression

Mammalian

Mutation

V5A

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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hKCNQ3(WT).ires.mCherry-pcDNA3

Plasmid

#204362

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ3 (KCNQ3 Human)

Expression

Mammalian

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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RUNX1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176974

Purpose

Mammalian expression vector encoding RUNX1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

RUNX1 (RUNX1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Mo-Trip6

Plasmid

#27254

Depositor

Thomas Gilmore

Article

Wang et al Gene. 1999 Jul 8. 234(2):403-9.

Insert

Trip6 (Trip6 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 3xF-dyskerin

Plasmid

#126870

Purpose

Expresses 3xFlag dsykerin

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

Dyskerin

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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