We narrowed to 23,118 results for: Sis
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Plasmid#82422PurposePlasmid for expression of NHA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cellsDepositorInsertnvGW182 NED
TagsNHAExpressionMammalianMutationdeleted amino acids 1159-1698 from nvGW182PromoterCMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1477
Plasmid#82423PurposePlasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED
TagsNHAExpressionMammalianMutationdeleted amino acids 1-1158 from nvGW182PromoterCMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1479
Plasmid#82425PurposePlasmid for expression of GST-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cellsDepositorInsertnvGW182 NED
TagsGSTExpressionMammalianMutationdeleted amino acids 1159-1698 from nvGW182PromoterEF1AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1480
Plasmid#82426PurposePlasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED
TagsGSTExpressionMammalianMutationamino acids 1-1158 of nvGW182 were deletedPromoterEF1AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA1_no stop
Plasmid#83847PurposeGateway donor vector for IAA1 with no stop codonDepositorAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_111del
Plasmid#65659PurposeFLAG-HA expression vector with RBPMS carrying deletion at amino acid 111 (1-111) and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationintroduced stop codon such as to produce deletion…PromoterCMV, 2x TetAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A
Plasmid#65660PurposeFLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchanged glutamic acid 97 to alanine, lysine 100 t…PromoterCMV, 2x TetAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D377Y-mPCSK9
Plasmid#58376PurposeExpresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorHas ServiceAAV8Insertproprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
UseAAVExpressionMammalianMutationD377YPromoterHCRApoE/hAATAvailable SinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7II-2N4Rtau
Plasmid#177653PurposeRecombinant protein synthesis of human TauDepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tn7 integration plasmid
Plasmid#154134PurposeSuicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.DepositorInsertsleft and right end of Tn7 arm
Spectinomycin resistant gene
ExpressionBacterialPromoterSpectinomycin resistance gene promoter and noAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBD-PYR1_star-MANDI
Plasmid#241280PurposeYeast two hybrid vector expressing BD-PYR1_star-MANDIDepositorAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSP90AA1
Plasmid#177658PurposeRecombinant protein synthesis of human HSP90AA1DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPA8
Plasmid#177661PurposeRecombinant protein synthesis of human HSPA8DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Frb-v1
Plasmid#201923PurposeE. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.DepositorInsertsFrbA
FrbB
FrbC
FrbD
FrbE
ExpressionBacterialPromoterT7 (modified), T7 (modified, moderate), T7 (modif…Available SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-Turbo-YFP-NLS
Plasmid#127368PurposeBinary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
TagsGS linker, NLS, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterArabidopsis UBQ10 promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB2B
Plasmid#195284PurposeBicistronic HSV-TK and Puromycin resistance for positive/negative selectionDepositorInsertHSV-TK 2A Puro
ExpressionMammalianPromoterPGKAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE5max-SF3B1-K700E
Plasmid#200317PurposeExpresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.DepositorInsertsK700E-ng1+pg2
PEmax-2A-MLH1dn
ExpressionMammalianPromoterCMVAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin1
Plasmid#124852PurposeMutagenesis of Grin1DepositorInsertGrin1 (Grin1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D374Y-hPCSK9
Plasmid#58379PurposeExpresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
UseAAVExpressionMammalianMutationD374YPromoterHCRApoE/hAATAvailable SinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
aMHC-mCherry-Rex-Blasticidin
Plasmid#21228DepositorInsertalpha myosin heavy chain promoter
UseLentiviralTagsmCherryAvailable SinceJune 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_ScaI_BFP
Plasmid#177824PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).DepositorInserttagBFP
ExpressionMammalianMutation5' inserted ScaI RE cut sitePromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgSlc17a6
Plasmid#124847PurposeMutagenesis of Slc17a6 with SauCas9DepositorInsertSlc17a6 gRNA (Slc17a6 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
AID-mTagBFP2-loxP_myo2_neoR-loxP
Plasmid#194055PurposeDual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genomeDepositorAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-Turbo-NES-YFP
Plasmid#127366PurposeBinary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
TagsGS linker, NES, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterArabidopsis UBQ10 promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGrin2b
Plasmid#124864PurposeMutagenesis of Grin2bDepositorInsertGrin2b (Grin2b Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB2F
Plasmid#195289PurposeBicistronic HSV-TK and mScarlet resistance for positive/negative selectionDepositorInsertHSV-TK 2A mScarlet
ExpressionMammalianPromoterPGKAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-miniTurbo-YFP-NLS
Plasmid#127370PurposeBinary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plantsDepositorInsertminiTurbo (BirA mutant)
TagsGS linker, NLS, V5, and YFPExpressionPlantMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterArabidopsis UBQ10 promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB2C
Plasmid#195285PurposeBicistronic HSV-TK and Hygromycin resistance for positive/negative selectionDepositorInsertHSV-TK 2A Hygro
ExpressionMammalianPromoterPGKAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSUMO1 GFP
Plasmid#118360PurposeThis plasmid expresses the full length human SUMO1.DepositorAvailable SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCdOpt-BMX
Plasmid#203929PurposeThis plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiaeDepositorInsertBleMX
ExpressionYeastMutationBleMX was codon optimized for Candida albicans or…Available SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-B-AtAFB2-mCherry
Plasmid#129718PurposeAAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherryDepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherryExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB2G
Plasmid#195290PurposeBicistronic HSV-TK and mTagBFP resistance for positive/negative selectionDepositorInsertHSV-TK 2A mTagBFP
ExpressionMammalianPromoterPGKAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
aMHC-eGFP-Rex-Neo
Plasmid#21229DepositorInsertalpha myosin heavy chain promoter
UseLentiviralTagseGFPAvailable SinceJune 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin2b
Plasmid#124851PurposeMutagenesis of Grin2bDepositorInsertGrin2b (Grin2b Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-miniTurbo-NES-YFP
Plasmid#127369PurposeBinary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plantsDepositorInsertminiTurbo (BirA mutant)
TagsGS linker, NES, V5, and YFPExpressionPlantMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterArabidopsis UBQ10 promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV306DIG13+FFlucRT
Plasmid#49998PurposeIntegrase removed to increase stability of the plasmid in mycobacteriaDepositorInsertG13 promoter + Firefly luciferase Red Thermostable
UseMycobacteria integrating vectorExpressionBacterialMutationCodon optimized for Mycobacterium tuberculosis wi…PromoterG13Available SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
CD8-mCherry-FRB
Plasmid#100738PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMV306G13+FFlucRT
Plasmid#49997PurposeIntegrating vector with an improved Red-shifted Thermostable Firefly LuciferaseDepositorInsertG13 promoter + Firefly luciferase Red Thermostable
UseMycobacteria integrating vectorExpressionBacterialMutationCodon optimized for Mycobacterium tuberculosis wi…PromoterG13 from M. marinumAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP-epsin(144-575)-GFP
Plasmid#100733PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertepn1 (Epn1 Mouse)
TagsFKBP and GFPExpressionMammalianMutationclathrin binding middle part aa 144-575PromoterCMVAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
aMHC-puro rex-neo
Plasmid#21230DepositorInsertalpha myosin heavy chain promoter
UseLentiviralAvailable SinceSept. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGabrg2
Plasmid#124857PurposeMutagenesis of Gabrg2DepositorInsertGabrg2 (Gabrg2 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana PHOT1
Plasmid#209187PurposepENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tagsDepositorAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_mPlum_C207G/T208C
Plasmid#177831PurposeSite-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).DepositorInsertmPlum_C207G/T208C
ExpressionMammalianMutationC207G + T208CPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-hHSF1 shRNA
Plasmid#118345PurposeThis plasmid expresses shRNA against hHSF1.DepositorAvailable SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc
Plasmid#208382PurposeDerived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.DepositorTypeEmpty backboneUseLentiviral and LuciferaseExpressionMammalianMutationWTAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBW_0065
Plasmid#102817PurposepVec8 containing Sr22 geneDepositorAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pBW_0141
Plasmid#102820PurposepVec8 containing Sr45 geneDepositorAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSUMO1-HIS
Plasmid#118361PurposeThis plasmid expresses the full length human SUMO1.DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only