We narrowed to 14,123 results for: cas9 genes
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pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179027PurposeParent vector for markerless yeast integration cassettes with untagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK45 pHomL-RPL18Bp-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179029PurposeParent vector for markerless yeast integration cassettes with untagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK44 pHomL-TEF2p-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179028PurposeParent vector for markerless yeast integration cassettes with untagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Zeo
Plasmid#92053PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRPromoterpRhamBAD and ptetAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Amp
Plasmid#92051PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRPromoterpRhamBAD and ptetAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Cm
Plasmid#92052PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRPromoterpRhamBAD and ptetAvailable SinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS212
Plasmid#87346PurposeCysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmidDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/C574SPromoterT7Available SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD121
Plasmid#91833PurposeCas9 expression in C. elegansDepositorInsertCas9
UseCRISPRExpressionWormMutationCodon optimized and with synthetic introns for C.…Available SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-hCASeGFP-pA
Plasmid#59766PurposeExpresses CAS9-eGFP fusion protein in mammalian cells.DepositorInsertCAS9
TagsFlag and eGFPExpressionMammalianPromoterCAGAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMZ288
Plasmid#59897PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI siteAvailable SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMZ289
Plasmid#59898PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI siteAvailable SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMZ381
Plasmid#59899PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI siteAvailable SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-2BD
Plasmid#234428PurposePlasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning templateDepositorInsertModified Cas9 with polyaspartate linking
ExpressionBacterialMutationLinked polyaspartate using glycine-serine linkerAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNA0306
Plasmid#169452PurposepRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmidDepositorInsertCas9
ExpressionYeastPromoterTEF1pAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry Codon 59 sgRNA
Plasmid#109431PurposeMLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporterDepositorInsertmCherry codon 59 gRNA
UseCRISPRExpressionMammalianPromoterhU6Available SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLS485
Plasmid#188890PurposeWorm Cas9 expression vector, Peft-3 promoterDepositorInsertPeft-3::Cas9
ExpressionWormMutationC. elegans codom optimization and intron additionAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
DS-SPcas
Plasmid#48645PurposeBacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
tracrRNA precursor
UseCRISPRPromoterproC and tracrRNA promoterAvailable SinceDec. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
M-ST1cas
Plasmid#48669PurposeMammalian S. thermophilus #1 Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSPromoterCMVAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
M-NMcas
Plasmid#48670PurposeMammalian N. meningitidis Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSPromoterCMVAvailable SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
DS-NMcas
Plasmid#48646PurposeBacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
NM tracrRNA
UseCRISPRPromoterNM tracrRNA promoter and proCAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
M-SPcas
Plasmid#48668PurposeMammalian S. pyogenes Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSPromoterCMVAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
DS-ST1casN-
Plasmid#48659PurposeBacterial nuclease-null ST1 Cas9 expressionDepositorInsertsCas9, nuclease-null
ST1 tracrRNA
UseCRISPRMutationD to A, DH to AA, N to APromoterproCAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
DS-ST1cas
Plasmid#48647PurposeBacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
ST1 tracrRNA
UseCRISPRPromoterproCAvailable SinceNov. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
DS-TDcasN-
Plasmid#48660PurposeBacterial nuclease-null TD Cas9 expressionDepositorInsertsCas9, nuclease-null
TD tracrRNA
UseCRISPRMutationD to A, DH to AA, N to APromoterproC and tracrRNA promoterAvailable SinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMLS544
Plasmid#188882PurposeIntegrating Pmex-5::Cas9 expression vectorDepositorInsertPmex-5::Cas9
ExpressionWormMutationC. elegans codom optimization and intron additionAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS546
Plasmid#188883PurposeIntegrating Pmex-5::Cas9 expression vectorDepositorInsertPmex-5::Cas9
ExpressionWormMutationC. elegans codom optimizationAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179012PurposeParent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB06
Plasmid#190480PurposeBase Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsCas9
XylR
UseE. coli / b. subtilis - c. difficile shuttle vect…PromoterxylR-driven promoterAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMB66
Plasmid#47946PurposeExpresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormPromoterhsp-16.48Available SinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMB67
Plasmid#47947PurposeExpresses C. elegans optimized Cas9 from the hsp-16.48 promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, and egl-13 NLSExpressionWormPromoterhsp-16.48Available SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBBK31 pHomL-TDH3p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179015PurposeParent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK26 pHomL-TEF2p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179010PurposeParent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK25 pHomL-TDH3p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179009PurposeParent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR)
Plasmid#179080PurposeParent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminatorsDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK42 pHomL-RPL18Bp-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)
Plasmid#179026PurposeParent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK41 pHomL-TEF2p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)
Plasmid#179025PurposeParent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK40 pHomL-TDH3p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)
Plasmid#179024PurposeParent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK39 pHomL-RPL18Bp-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179023PurposeParent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK38 pHomL-TEF2p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179022PurposeParent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK37 pHomL-TDH3p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179021PurposeParent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK36 pHomL-RPL18Bp-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179020PurposeParent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK35 pHomL-TEF2p-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179019PurposeParent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK34 pHomL-TDH3p-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179018PurposeParent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK33 pHomL-RPL18Bp-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179017PurposeParent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK32 pHomL-TEF2p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179016PurposeParent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK30 pHomL-RPL18Bp-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179014PurposeParent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK29 pHomL-TEF2p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179013PurposeParent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK27 pHomL-RPL18Bp-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179011PurposeParent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLSC-5
Plasmid#62889PurposeLenti vector for expression of inducible split Cas9DepositorInsertsSpCas9(574-1368)
SpCas9(2-573)
UseCRISPR and LentiviralTagsFFBP12, FRB, NES PTK2, NLS SV40, and P2AExpressionMammalianMutation*see comment belowPromoterEFSAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only
